异槲皮素对小鼠卵巢切除所致骨质疏松症的治疗作用

IF 4.8 3区 化学 Q1 CHEMISTRY, MEDICINAL
Mengjing Wu, Mengyu Qin, Xian Wang
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引用次数: 0

摘要

骨髓间充质干细胞(BMSCs)是一种能够分化为成熟细胞的非造血多能干细胞。异槲皮素是一种天然提取物,有望成为治疗骨质疏松症的潜在药物。为了研究异槲皮素对骨质疏松症的治疗作用,体外培养骨髓间充质干细胞(BMSCs),并在异槲皮素存在下诱导成骨或脂肪形成14天。我们评估了细胞活力、成骨和脂肪分化,以及成骨细胞中Runx2、Alpl和OCN的mRNA表达水平,以及脂肪细胞中Pparγ、Fabp4和Cebpα的mRNA表达水平。结果显示,异槲皮素剂量依赖性地提高细胞活力,促进成骨分化,茜素红、碱性磷酸酶染色及成骨细胞Runx2、Alpl、OCN mRNA表达水平均证实了异槲皮素的作用(P < 0.05)。相反,异槲皮素抑制脂肪细胞成脂分化,降低脂肪细胞中Pparγ、Fabp4和Cebpα mRNA表达水平(P < 0.05)。在体内,异槲皮素处理增加了骨质疏松模型小鼠组的骨量和骨密度,通过μCT扫描和免疫组化(P < 0.05)。这些发现表明,异槲皮素可能通过促进骨髓间充质干细胞向成骨细胞的增殖和分化,同时抑制成脂分化,从而具有治疗骨质疏松症的潜力。图形抽象
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Therapeutic effects of isoquercetin on ovariectomy-induced osteoporosis in mice

Therapeutic effects of isoquercetin on ovariectomy-induced osteoporosis in mice

Bone marrow mesenchymal stem cells (BMSCs) are non-hematopoietic multipotent stem cells capable of differentiating into mature cells. Isoquercetin, an extract from natural sources, has shown promise as a potential treatment for osteoporosis. To investigate the therapeutic effects of isoquercetin on osteoporosis, bone marrow mesenchymal stem cells (BMSCs) were cultured in vitro, and osteogenesis or adipogenesis was induced in the presence of isoquercetin for 14 days. We evaluated cell viability, osteogenic and adipogenic differentiation, as well as mRNA expression levels of Runx2, Alpl, and OCN in osteoblasts, and mRNA expression levels of Pparγ, Fabp4, and Cebpα in adipocytes. The results showed that isoquercetin dose-dependently increased cell viability and promoted osteogenic differentiation, as evidenced by Alizarin Red and alkaline phosphatase staining and mRNA expression levels of Runx2, Alpl, and OCN in osteoblasts (P < 0.05). In contrast, isoquercetin inhibited adipogenic differentiation and decreased the mRNA expression levels of Pparγ, Fabp4, and Cebpα in adipocytes (P < 0.05). In vivo, isoquercetin treatment increased bone quantity and density in an osteoporosis model mice group, as determined by μCT scanning and immunohistochemistry (P < 0.05). These findings suggest that isoquercetin may have therapeutic potential for osteoporosis by promoting the proliferation and differentiation of BMSCs towards osteoblasts while inhibiting adipogenic differentiation.

Graphical Abstract

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来源期刊
Natural Products and Bioprospecting
Natural Products and Bioprospecting CHEMISTRY, MEDICINAL-
CiteScore
8.30
自引率
2.10%
发文量
39
审稿时长
13 weeks
期刊介绍: Natural Products and Bioprospecting serves as an international forum for essential research on natural products and focuses on, but is not limited to, the following aspects: Natural products: isolation and structure elucidation Natural products: synthesis Biological evaluation of biologically active natural products Bioorganic and medicinal chemistry Biosynthesis and microbiological transformation Fermentation and plant tissue cultures Bioprospecting of natural products from natural resources All research articles published in this journal have undergone rigorous peer review. In addition to original research articles, Natural Products and Bioprospecting publishes reviews and short communications, aiming to rapidly disseminate the research results of timely interest, and comprehensive reviews of emerging topics in all the areas of natural products. It is also an open access journal, which provides free access to its articles to anyone, anywhere.
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