富含多酚的甘蔗提取物对人单核细胞的免疫调节特性

J. Feehan, M. Prakash, L. Stojanovska, M. Flavel, B. Kitchen, V. Apostolopoulos
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引用次数: 2

摘要

随着炎症生活方式因素的日益普遍和人口的老龄化,炎症的管理将变得越来越重要。植物多酚是一种强大的抗氧化剂,已知对许多具有炎症或氧化成分的疾病具有有益作用,如恶性肿瘤、心血管疾病和关节炎。富含多酚的甘蔗提取物(PRSE)是一种具有高浓度多酚抗氧化剂的新制剂,有一些证据表明其对健康有益,但研究其对免疫调节作用的研究有限。本研究测定了PRSE对体外培养的人单核细胞的影响。我们发现,PRSE在U937人单核细胞中具有免疫调节作用,改变了细胞表面标志物的表达,CD16和CD11b的表达增加,CD40、CD80、CD206和MHCI也发生了微小变化。它还调节分泌的细胞因子,增加IL-1β、TNFα、IL-6、IL-8、IL-4和IL-10。这些变化与单核细胞的高级分化以及巨噬细胞从M1表型向M2表型的转变一致。我们还证明,这种作用可能独立于NF-κB信号通路,这表明其他机制驱动了这种作用。PRSE在体外对U937单核细胞发挥免疫调节作用,可能促进炎症向愈合的转化。未来的研究应该确定这些变化的具体机制,并评估它们在疾病动物模型中的有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunomodulatory Properties of Polyphenol-Rich Sugarcane Extract on Human Monocytes
As inflammatory lifestyle factors become more prevalent and as the population ages, the management of inflammation will become increasingly relevant. Plant polyphenols are powerful antioxidants that are known to have beneficial effects in a number of diseases with an inflammatory or oxidative component, such as malignancy, cardiovascular disease and arthritis. Polyphenol-rich sugarcane extract (PRSE) is a novel preparation with high concentrations of polyphenolic antioxidants, with some evidence to show benefits in health, but there is limited research investigating its effects on immunomodulation. This study determined the effects of PRSE on human monocyte cells in vitro. We show that PRSE has an immunomodulatory effect in U937 human monocyte cells, altering the expression of cellular surface markers, with an increased expression of CD16 and CD11b, as well as small changes in CD40, CD80, CD80, CD206 and MHCI. It also modulates the profile of secreted cytokines, increasing IL-1β, TNFα, IL-6, IL-8, IL-4 and IL-10. These changes are consistent with the advanced differentiation of the monocyte, as well as the switch from the M1 to M2 phenotype in macrophages. We also demonstrate that this effect is likely to be independent of the NF-κB signalling pathway, suggesting that other mechanisms drive this effect. PRSE exerts an immunomodulatory effect on U937 monocytes in vitro, potentially facilitating the conversion from inflammation to healing. Future studies should identify specific mechanisms underlying the changes and evaluate their effectiveness in animal models of disease.
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