{"title":"离子液体对脂质体理化特性和毒理学影响的研究","authors":"Manas Kumar Mandal , Emili Manna , Habiba Sultana , Manas Barai , Kartik Chandra Guchhait , Chandradipa Ghosh , Anuttam Patra , Kaushik Nag , Shin-ichi Yusa , Amiya Kumar Panda","doi":"10.1016/j.jciso.2022.100050","DOIUrl":null,"url":null,"abstract":"<div><p>Effect of 1-butyl-3-methylimidazolium chloride ionic liquid ([bmim]Cl, IL) on the monolayer/bilayer of either soy-phosphatidylcholine (SPC) or hydrogenated soy-phosphatidylcholine (HSPC), in combination with 30 mol% cholesterol (Chol), were investigated. Impact of IL on monolayers were explored by measuring the surface pressure (<em>π</em>)-area (<em>A</em>) isotherm with a Langmuir-surface balance. Lift-off area (<em>A</em><sub>0</sub>) of the monomolecular films gradually increased [<em>A</em><sub>0(HSPC+IL)</sub> > <em>A</em><sub>0(SPC+IL)</sub>], collapse pressures (<em>π</em><sub>c</sub>) decreased and passed through minima [<em>π</em><sub>c(HSPC+IL)</sub> > <em>π</em><sub>c(SPC+IL)</sub>] with increasing IL concentration ([IL]). Minimum molecular area (<em>A</em><sub>min</sub>) increased monotonously and compression moduli (<em>C</em><sub><em>s</em></sub><sup>−1</sup>) followed the sequence (HSPC+IL) > (SPC+IL) at a particular <em>π</em> with respect to [IL]. Dynamic light scattering studies were carried out to determine the hydrodynamic diameter (<em>d</em><sub>h</sub>), zeta potential (<em>Z.P.</em>) and polydispersity index (<em>PDI</em>) values while fluorescence anisotropy studies, using 7-hydroxycoumarin and 1,6-diphenyl-1,3,5-hexatriene, could reveal the micro-viscosity of liposomes. Increased size and rigidity, induced by IL, suggest the formation of leak-proof, condensed liposomes. Disruption of vesicles induced by IL were observed from transmission electron microscopic (TEM) studies. IL induced disintegration of liposome and kinetics of subsequent formation of adsorbed monolayer were accomplished by surface pressure-time isotherms. IL-induced liposomes were substantially less toxic as revealed by MTT assay. These liposomes are considered to be safely used as effective and controlled drug delivery systems.</p></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666934X22000083/pdfft?md5=b3c031b8bc57faa170728c813422837f&pid=1-s2.0-S2666934X22000083-main.pdf","citationCount":"1","resultStr":"{\"title\":\"Investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomes\",\"authors\":\"Manas Kumar Mandal , Emili Manna , Habiba Sultana , Manas Barai , Kartik Chandra Guchhait , Chandradipa Ghosh , Anuttam Patra , Kaushik Nag , Shin-ichi Yusa , Amiya Kumar Panda\",\"doi\":\"10.1016/j.jciso.2022.100050\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Effect of 1-butyl-3-methylimidazolium chloride ionic liquid ([bmim]Cl, IL) on the monolayer/bilayer of either soy-phosphatidylcholine (SPC) or hydrogenated soy-phosphatidylcholine (HSPC), in combination with 30 mol% cholesterol (Chol), were investigated. Impact of IL on monolayers were explored by measuring the surface pressure (<em>π</em>)-area (<em>A</em>) isotherm with a Langmuir-surface balance. Lift-off area (<em>A</em><sub>0</sub>) of the monomolecular films gradually increased [<em>A</em><sub>0(HSPC+IL)</sub> > <em>A</em><sub>0(SPC+IL)</sub>], collapse pressures (<em>π</em><sub>c</sub>) decreased and passed through minima [<em>π</em><sub>c(HSPC+IL)</sub> > <em>π</em><sub>c(SPC+IL)</sub>] with increasing IL concentration ([IL]). Minimum molecular area (<em>A</em><sub>min</sub>) increased monotonously and compression moduli (<em>C</em><sub><em>s</em></sub><sup>−1</sup>) followed the sequence (HSPC+IL) > (SPC+IL) at a particular <em>π</em> with respect to [IL]. Dynamic light scattering studies were carried out to determine the hydrodynamic diameter (<em>d</em><sub>h</sub>), zeta potential (<em>Z.P.</em>) and polydispersity index (<em>PDI</em>) values while fluorescence anisotropy studies, using 7-hydroxycoumarin and 1,6-diphenyl-1,3,5-hexatriene, could reveal the micro-viscosity of liposomes. Increased size and rigidity, induced by IL, suggest the formation of leak-proof, condensed liposomes. Disruption of vesicles induced by IL were observed from transmission electron microscopic (TEM) studies. IL induced disintegration of liposome and kinetics of subsequent formation of adsorbed monolayer were accomplished by surface pressure-time isotherms. IL-induced liposomes were substantially less toxic as revealed by MTT assay. These liposomes are considered to be safely used as effective and controlled drug delivery systems.</p></div>\",\"PeriodicalId\":73541,\"journal\":{\"name\":\"JCIS open\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2666934X22000083/pdfft?md5=b3c031b8bc57faa170728c813422837f&pid=1-s2.0-S2666934X22000083-main.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"JCIS open\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666934X22000083\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Materials Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"JCIS open","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666934X22000083","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Materials Science","Score":null,"Total":0}
Investigations on the role of ionic liquid on the physicochemical characteristics and toxicological consequences of liposomes
Effect of 1-butyl-3-methylimidazolium chloride ionic liquid ([bmim]Cl, IL) on the monolayer/bilayer of either soy-phosphatidylcholine (SPC) or hydrogenated soy-phosphatidylcholine (HSPC), in combination with 30 mol% cholesterol (Chol), were investigated. Impact of IL on monolayers were explored by measuring the surface pressure (π)-area (A) isotherm with a Langmuir-surface balance. Lift-off area (A0) of the monomolecular films gradually increased [A0(HSPC+IL) > A0(SPC+IL)], collapse pressures (πc) decreased and passed through minima [πc(HSPC+IL) > πc(SPC+IL)] with increasing IL concentration ([IL]). Minimum molecular area (Amin) increased monotonously and compression moduli (Cs−1) followed the sequence (HSPC+IL) > (SPC+IL) at a particular π with respect to [IL]. Dynamic light scattering studies were carried out to determine the hydrodynamic diameter (dh), zeta potential (Z.P.) and polydispersity index (PDI) values while fluorescence anisotropy studies, using 7-hydroxycoumarin and 1,6-diphenyl-1,3,5-hexatriene, could reveal the micro-viscosity of liposomes. Increased size and rigidity, induced by IL, suggest the formation of leak-proof, condensed liposomes. Disruption of vesicles induced by IL were observed from transmission electron microscopic (TEM) studies. IL induced disintegration of liposome and kinetics of subsequent formation of adsorbed monolayer were accomplished by surface pressure-time isotherms. IL-induced liposomes were substantially less toxic as revealed by MTT assay. These liposomes are considered to be safely used as effective and controlled drug delivery systems.
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