澳大利亚足球运动员脑震荡后血浆miR-27a和miR-221的减少

IF 3.4 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
S. Shultz, Caroline J Taylor, Riemke Aggio-Bruce, W. T. O'Brien, Mujun Sun, Adrian V Cioanca, George A. Neocleous, Georgia F Symons, R. Brady, A. Hardikar, M. Joglekar, Daniel M Costello, T. O’Brien, R. Natoli, S. McDonald
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引用次数: 6

摘要

运动相关脑震荡(SRC)是一种常见的脑损伤形式,缺乏可靠的方法来指导临床决策。microrna (mirna)可以影响SRC中涉及的生物过程,测量生物体液中的mirna可以提供客观的诊断和回归/恢复生物标志物。因此,这项前瞻性研究调查了脑震荡男女运动员循环miRNA水平的时间分布。方法:采集业余澳式足球运动员季前基线血液样本(男82人,女45人)。其中,20只雄性和8只雌性在随后的季节中持续了SRC,并在受伤后2、6和13天进行了血液采样。在血浆中使用miRNA发现开放阵列来评估754个已知/验证的miRNA的表达。在验证研究中,我们用实时荧光定量PCR (qRT-PCR)进一步研究鉴定出的miRNA靶点。还收集了与SRC症状、人口统计学、运动史、教育史和脑震荡史有关的数据。结果:发现分析鉴定出18个候选miRNA。随后的验证研究发现,与基线相比,血浆miR-221-3p水平在第6天和第13天降低,miR-27a-3p水平在第6天降低。此外,miR-27a和miR-221-3p水平与SRC症状严重程度呈负相关。结论:外周血miR-27a-3p和miR-221-3p水平在SRC后亚急性期降低,且与SRC症状严重程度呈负相关。虽然需要进一步的研究,但这些分析已经确定了SRC严重程度和恢复的miRNA生物标志物候选物,可能有一天有助于其临床管理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Decrease in Plasma miR-27a and miR-221 After Concussion in Australian Football Players
Introduction: Sports-related concussion (SRC) is a common form of brain injury that lacks reliable methods to guide clinical decisions. MicroRNAs (miRNAs) can influence biological processes involved in SRC, and measurement of miRNAs in biological fluids may provide objective diagnostic and return to play/recovery biomarkers. Therefore, this prospective study investigated the temporal profile of circulating miRNA levels in concussed male and female athletes. Methods: Pre-season baseline blood samples were collected from amateur Australian rules football players (82 males, 45 females). Of these, 20 males and 8 females sustained an SRC during the subsequent season and underwent blood sampling at 2-, 6- and 13-days post-injury. A miRNA discovery Open Array was conducted on plasma to assess the expression of 754 known/validated miRNAs. miRNA target identified were further investigated with quantitative real-time PCR (qRT-PCR) in a validation study. Data pertaining to SRC symptoms, demographics, sporting history, education history and concussion history were also collected. Results: Discovery analysis identified 18 candidate miRNA. The consequent validation study found that plasma miR-221-3p levels were decreased at 6d and 13d, and that miR-27a-3p levels were decreased at 6d, when compared to baseline. Moreover, miR-27a and miR-221-3p levels were inversely correlated with SRC symptom severity. Conclusion: Circulating levels of miR-27a-3p and miR-221-3p were decreased in the sub-acute stages after SRC, and were inversely correlated with SRC symptom severity. Although further studies are required, these analyses have identified miRNA biomarker candidates of SRC severity and recovery that may one day assist in its clinical management.
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来源期刊
Biomarker Insights
Biomarker Insights MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.00
自引率
0.00%
发文量
26
审稿时长
8 weeks
期刊介绍: An open access, peer reviewed electronic journal that covers all aspects of biomarker research and clinical applications.
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