Lizeth Paola Sánchez, Miguel Ángel Martínez, T. León, Tatiana Cordoba, María Calvo, L. Montaño, Patricia Escandón, Silvia Narváez, Janet Vivas, Magdalena Wiesner
{"title":"4种弧菌多重PCR检测方法的建立与实施","authors":"Lizeth Paola Sánchez, Miguel Ángel Martínez, T. León, Tatiana Cordoba, María Calvo, L. Montaño, Patricia Escandón, Silvia Narváez, Janet Vivas, Magdalena Wiesner","doi":"10.22354/24223794.1047","DOIUrl":null,"url":null,"abstract":"Aim: Different species of the genus Vibrio are pathogenic for humans, the aim of this study was to standardize a multiplex PCR to identify four Vibrio species. Methods. 126 isolates environmental and 66 from the surveillance program INS were analyzed by PCR species-specific genes. Results. Of the total isolates, 124 were PCR positive for one of the species-specific genes. The PCR achieved the identification of the four target species (V. parahaemolyticus, V. alginolyticus, V. vulnificus and V. fluvialis), showing specificity and sensitivity between 1-2 ng/ul. Discussion. A multiplex PCR was developed that could provide reliable identification of four Vibrio species.","PeriodicalId":38132,"journal":{"name":"Infectio","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and implementation of a multiplex PCR for the detection of 4 species of Vibrio\",\"authors\":\"Lizeth Paola Sánchez, Miguel Ángel Martínez, T. León, Tatiana Cordoba, María Calvo, L. Montaño, Patricia Escandón, Silvia Narváez, Janet Vivas, Magdalena Wiesner\",\"doi\":\"10.22354/24223794.1047\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aim: Different species of the genus Vibrio are pathogenic for humans, the aim of this study was to standardize a multiplex PCR to identify four Vibrio species. Methods. 126 isolates environmental and 66 from the surveillance program INS were analyzed by PCR species-specific genes. Results. Of the total isolates, 124 were PCR positive for one of the species-specific genes. The PCR achieved the identification of the four target species (V. parahaemolyticus, V. alginolyticus, V. vulnificus and V. fluvialis), showing specificity and sensitivity between 1-2 ng/ul. Discussion. A multiplex PCR was developed that could provide reliable identification of four Vibrio species.\",\"PeriodicalId\":38132,\"journal\":{\"name\":\"Infectio\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-07-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infectio\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22354/24223794.1047\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infectio","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22354/24223794.1047","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Development and implementation of a multiplex PCR for the detection of 4 species of Vibrio
Aim: Different species of the genus Vibrio are pathogenic for humans, the aim of this study was to standardize a multiplex PCR to identify four Vibrio species. Methods. 126 isolates environmental and 66 from the surveillance program INS were analyzed by PCR species-specific genes. Results. Of the total isolates, 124 were PCR positive for one of the species-specific genes. The PCR achieved the identification of the four target species (V. parahaemolyticus, V. alginolyticus, V. vulnificus and V. fluvialis), showing specificity and sensitivity between 1-2 ng/ul. Discussion. A multiplex PCR was developed that could provide reliable identification of four Vibrio species.
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