UPLC-MS/MS法研究curdione在小鼠体内的药代动力学和生物利用度

IF 1.7 4区化学 Q3 CHEMISTRY, ANALYTICAL

Acta Chromatographica Pub Date : 2022-05-13 DOI:10.1556/1326.2022.01020

Qishun Liang, Tianyu Chen, Lvqi Luo, Yizhe Ma, Congcong Wen, Xueli Huang

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引用次数: 0

摘要

建立了UPLC-MS/MS法测定小鼠血液中的curdione，并研究了静脉(5 mg kg - 1)和口服(20 mg kg - 1)给药后curdione在小鼠体内的药代动力学。采用HSS T3柱进行分离，柱温为40℃。采用多反应监测(MRM)法测定莪术酮的含量。在给药后从癌症研究所(ICR)小鼠尾静脉采血。在1 ~ 500 ng mL−1范围内呈良好的线性关系(r为0 0.998);日内精密度为77%，基质效应为97% ~ 107%。半衰期较短，生物利用度为6.5%。该方法适用于莪术酮在小鼠体内的药动学研究。

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Pharmacokinetics and bioavailability of curdione in mice by UPLC-MS/MS

A UPLC-MS/MS method was developed to determinate curdione in the mouse blood, and the pharmacokinetics of curdione in mice after intravenous (5 mg kg−1) and oral (20 mg kg−1) administration were studied. The HSS T3 column was used for separation, and column temperature was set at 40 °C. Multiple reaction monitoring (MRM) mode were used for determination of curdione. Blood samples were taken from the caudal vein of Institute of Cancer Research (ICR) mice after administration of curdione. It showed a good linear relationship in the range of 1–500 ng mL−1 (r > 0.998); the intra-day precision was <13%, the inter-day precision was <15%, and the accuracy was 90%–105%, the recovery was >77%, and the matrix effect was 97%–107%. The half-life was relatively short, and the bioavailability was 6.5%. The developed method was suitable for the pharmacokinetics of curdione in mice.

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来源期刊

Acta Chromatographica 化学-分析化学

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

2.3 months

期刊介绍： Acta Chromatographica Open Access Acta Chromatographica publishes peer-reviewed scientific articles on every field of chromatography, including theory of chromatography; progress in synthesis and characterization of new stationary phases; chromatography of organic, inorganic and complex compounds; enantioseparation and chromatography of chiral compounds; applications of chromatography in biology, pharmacy, medicine, and food analysis; environmental applications of chromatography; analytical and physico-chemical aspects of sample preparation for chromatography; hyphenated and combined techniques; chemometrics and its applications in separation science.