用细胞毒性试验方法评价活性喷砂和喷砂表面的种植牙固定装置的骨和牙龈的活力和细胞增殖

IF 0.5 Q4 ENGINEERING, BIOMEDICAL
B. Mohammadi, E. Anbarzadeh
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引用次数: 3

摘要

近年来,种植牙技术进步很大,这已成为牙医在拔牙后立即提及种植牙的主要原因之一。在植入式研究中评估细胞存活率以确定细胞敏感性决定了治疗的结果。本研究旨在研究植入物表面与颌骨之间的骨整合特性以及细胞毒性。在本研究中,使用细胞毒性试验来评估喷砂大颗粒酸蚀刻的毒性和生存能力,并讨论了3A品牌种植牙中G292成骨细胞和人牙龈成纤维细胞的喷砂大颗粒酸性蚀刻活性表面。该操作使用德国MEMMERT公司的实验室培养箱进行24小时,在37°C的温度、1个大气压和90%的湿度下,通过neubauer lam细胞计数每个测试中的10万个培养细胞。根据扫描电子显微镜图像和细胞毒性测试结果,可以看出,采用喷砂大粒酸蚀活性表面处理的植入物的骨移植效果要好得多,也比采用喷砂大粒度酸蚀的植入物快一周。然而,对于G292成骨细胞和人牙龈成纤维细胞样品,采用喷砂大粒度酸蚀刻活性表面处理的植入物的生存能力分别等于98.4%和97.3%,并且低于喷砂大粒度酸性蚀刻表面处理。结果表明,在所有样品中,喷砂大粒度酸蚀植入物的生存能力比喷砂大粒度酸性蚀刻活性植入物高出约1.5%至2%,但喷砂大粒度活性植入物的表面完整性优于喷砂大粒度,处理过程缩短了一周。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of Viability and Cell Proliferation in Bone and Gingival on Dental Implant Fixtures with Active Sandblasted and Sandblasted Surfaces by the Cytotoxicity Test Method
In recent years, the technology of dental implants has advanced a lot and this has become one of the main reasons for dentists to refer to implants immediately after tooth extraction. Evaluation of cell survival in implantology studies to determine cell sensitivity determines the outcome of treatment. This study aimed to investigate the bone integration properties as well as the cytotoxicity between the implant surface and the jaw bone. In this study, cytotoxicity test was used to evaluate the toxicity and viability of sandblasted large grit acid etched, and sandblasted large grit acid etched active surfaces in 3A brand dental implants with G292 osteoblastic cells and human gingival fibroblasts cells are discussed. This operation was performed using a laboratory incubator of the German company MEMMERT for 24 hours, by neubauer lam cells counting for one hundred thousand cultured cells in each test at a temperature of 37 °C, a pressure of 1 atmosphere and 90% humidity. Based on the scanning electron microscopy images and the cytotoxicity test results, it can be seen that the bone graft of the implant, with the sandblasted large grit acid etched active surface treatment, is much better and also one week faster than the implant with the sandblasted large grit acid etched one. However, the viability of the implant with the sandblasted large grit acid etched active surface treatment for both G292 osteoblastic cells and human gingival fibroblasts cells samples was equal to 98.4% and 97.3%, respectively, and is lower than the sandblasted large grit acid etched surface treatment. The results show that the viability of the sandblasted large grit acid etched implant is about 1.5 to 2% higher than the sandblasted large grit acid etched active one, but the surface integrity of sandblasted large grit acid etched active is better than sandblasted large grit acid etched in all samples, and the treatment process is reduced by one week.
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来源期刊
CiteScore
1.40
自引率
14.30%
发文量
73
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