Yao Junhong , Jiang Qiudong , Cai Hao , Zhu Xuemin , Ma Min , Duan Jinao , Dong Jie , Chen Jun
{"title":"中药温性内药精油的细胞毒性及增透活性","authors":"Yao Junhong , Jiang Qiudong , Cai Hao , Zhu Xuemin , Ma Min , Duan Jinao , Dong Jie , Chen Jun","doi":"10.1016/j.jtcm.2018.03.006","DOIUrl":null,"url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate on the cytotoxicity and penetration enhancement effect of essential oils (EOs) from warming the interior medicinals (WIM) from Traditional Chinese Medicine (TCM).</p></div><div><h3>METHODS</h3><p>EOs were extracted from WIM of Bichengqie (<em>Litseae Fructus</em>), Dingxiang (<em>Flos Syzygii Aromatici</em>), Huajiao (<em>Pericarpium Zanthoxyli Bungeani</em>), and Xiaohuixiang (<em>Fructus Foeniculi</em>) with warm nature, and Ganjiang (<em>Rhizoma Zingiberis</em>), Gaoliangjiang (<em>Rhizoma Alpiniae Officinari</em>), Rougui (<em>Cortex Cinnamomi Cassiae</em>), and Wuzhuyu (<em>Fructus Evodiae Rutaecarpae</em>) with hot nature, respectively. Their chemical compositions were analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxicity of the extracted eight EOs on HaCaT cells was measured and compared. Moreover, analyses of cell cycle and cell apoptosis were performed to investigate the cytotoxic mechanism. The transdermal penetration enhancement effects of the extracted eight EOs on ibuprofen were further compared by the modified Franz diffusion cell method.</p></div><div><h3>RESULTS</h3><p>The most abundant constituents in the extracted eight EOs were determined to be monoterpenes, especially oxygen-containing monoterpenes. The HaCaT cell cytotoxicity of EOs from WIM with hot nature were significantly (<em>P</em> = 0.020) higher than that with warm nature. Both ginger oil and zanthoxylum oil significantly induced G0/G1 phase arrestment in HaCaT cell cycle. For ginger oil from WIM with hot nature and zanthoxylum oil from WIM with warm nature, the main mechanisms of the cytotoxicity were found to be the induction of cellular necrosis and the cellular apoptosis, respectively. Furthermore, most of the tested EOs showed remarkable penetration enhancement activity on ibuprofen. However, no statistical significance (<em>P</em> = 0.18) was found between penetration enhancement activity of EOs from WIM with warm nature and EOs from WIM with hot nature.</p></div><div><h3>CONCLUSION</h3><p>With the enhanced penetration activity, the extracted EOs from the WIM demonstrated their significant effect of the cytotoxicity on the skin cells.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.03.006","citationCount":"3","resultStr":"{\"title\":\"Cytotoxicity and penetration enhancement activity of essential oils from warming the interior medicinals with hot or warm property in terms of Traditional Chinese Medicine\",\"authors\":\"Yao Junhong , Jiang Qiudong , Cai Hao , Zhu Xuemin , Ma Min , Duan Jinao , Dong Jie , Chen Jun\",\"doi\":\"10.1016/j.jtcm.2018.03.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>OBJECTIVE</h3><p>To investigate on the cytotoxicity and penetration enhancement effect of essential oils (EOs) from warming the interior medicinals (WIM) from Traditional Chinese Medicine (TCM).</p></div><div><h3>METHODS</h3><p>EOs were extracted from WIM of Bichengqie (<em>Litseae Fructus</em>), Dingxiang (<em>Flos Syzygii Aromatici</em>), Huajiao (<em>Pericarpium Zanthoxyli Bungeani</em>), and Xiaohuixiang (<em>Fructus Foeniculi</em>) with warm nature, and Ganjiang (<em>Rhizoma Zingiberis</em>), Gaoliangjiang (<em>Rhizoma Alpiniae Officinari</em>), Rougui (<em>Cortex Cinnamomi Cassiae</em>), and Wuzhuyu (<em>Fructus Evodiae Rutaecarpae</em>) with hot nature, respectively. Their chemical compositions were analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxicity of the extracted eight EOs on HaCaT cells was measured and compared. Moreover, analyses of cell cycle and cell apoptosis were performed to investigate the cytotoxic mechanism. The transdermal penetration enhancement effects of the extracted eight EOs on ibuprofen were further compared by the modified Franz diffusion cell method.</p></div><div><h3>RESULTS</h3><p>The most abundant constituents in the extracted eight EOs were determined to be monoterpenes, especially oxygen-containing monoterpenes. The HaCaT cell cytotoxicity of EOs from WIM with hot nature were significantly (<em>P</em> = 0.020) higher than that with warm nature. Both ginger oil and zanthoxylum oil significantly induced G0/G1 phase arrestment in HaCaT cell cycle. For ginger oil from WIM with hot nature and zanthoxylum oil from WIM with warm nature, the main mechanisms of the cytotoxicity were found to be the induction of cellular necrosis and the cellular apoptosis, respectively. Furthermore, most of the tested EOs showed remarkable penetration enhancement activity on ibuprofen. However, no statistical significance (<em>P</em> = 0.18) was found between penetration enhancement activity of EOs from WIM with warm nature and EOs from WIM with hot nature.</p></div><div><h3>CONCLUSION</h3><p>With the enhanced penetration activity, the extracted EOs from the WIM demonstrated their significant effect of the cytotoxicity on the skin cells.</p></div>\",\"PeriodicalId\":17513,\"journal\":{\"name\":\"Journal of Traditional Chinese Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2018-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.jtcm.2018.03.006\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Traditional Chinese Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0254627218302516\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Traditional Chinese Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254627218302516","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
Cytotoxicity and penetration enhancement activity of essential oils from warming the interior medicinals with hot or warm property in terms of Traditional Chinese Medicine
OBJECTIVE
To investigate on the cytotoxicity and penetration enhancement effect of essential oils (EOs) from warming the interior medicinals (WIM) from Traditional Chinese Medicine (TCM).
METHODS
EOs were extracted from WIM of Bichengqie (Litseae Fructus), Dingxiang (Flos Syzygii Aromatici), Huajiao (Pericarpium Zanthoxyli Bungeani), and Xiaohuixiang (Fructus Foeniculi) with warm nature, and Ganjiang (Rhizoma Zingiberis), Gaoliangjiang (Rhizoma Alpiniae Officinari), Rougui (Cortex Cinnamomi Cassiae), and Wuzhuyu (Fructus Evodiae Rutaecarpae) with hot nature, respectively. Their chemical compositions were analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxicity of the extracted eight EOs on HaCaT cells was measured and compared. Moreover, analyses of cell cycle and cell apoptosis were performed to investigate the cytotoxic mechanism. The transdermal penetration enhancement effects of the extracted eight EOs on ibuprofen were further compared by the modified Franz diffusion cell method.
RESULTS
The most abundant constituents in the extracted eight EOs were determined to be monoterpenes, especially oxygen-containing monoterpenes. The HaCaT cell cytotoxicity of EOs from WIM with hot nature were significantly (P = 0.020) higher than that with warm nature. Both ginger oil and zanthoxylum oil significantly induced G0/G1 phase arrestment in HaCaT cell cycle. For ginger oil from WIM with hot nature and zanthoxylum oil from WIM with warm nature, the main mechanisms of the cytotoxicity were found to be the induction of cellular necrosis and the cellular apoptosis, respectively. Furthermore, most of the tested EOs showed remarkable penetration enhancement activity on ibuprofen. However, no statistical significance (P = 0.18) was found between penetration enhancement activity of EOs from WIM with warm nature and EOs from WIM with hot nature.
CONCLUSION
With the enhanced penetration activity, the extracted EOs from the WIM demonstrated their significant effect of the cytotoxicity on the skin cells.
期刊介绍：
Journal of Traditional Chinese Medicine（JTCM） is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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