{"title":"6株巴氏醋酸杆菌对RAW-Blue巨噬细胞的免疫调节作用","authors":"S. Kim, W. Jeong, So-Young Kim, S. Yeo","doi":"10.11002/kjfp.2023.30.1.65","DOIUrl":null,"url":null,"abstract":"\n \n In this study, we investigated the immunological properties of six strains of\n Acetobacter pasteurianus through nuclear factor-kappa\n B/activator protein-1 (NF-κB/AP-1) transcription factor activation and\n nitric oxide (NO) and cytokine production in macrophages. We found that the six\n A. pasteurianus strains had no significant inhibitory\n effect on the cell viability of RAW-Blue™ cells at the concentration of\n (25, 50, 100 CFU/macrophage). The production of NO and cytokines (TNF-α,\n IL-1β, and IL-6) showed different abilities of immune activation for each\n strain, and it was 0.7 to 0.9 times higher than that of the LPS (100 ng/mL, v/v)\n positive control and 7 to 8 times superior to that of the negative control\n group. To explore the underlying mechanism, we evaluated the mRNA expression of\n pro-inflammatory genes. Consequently, we found that inducible nitric oxide\n synthase (iNOS) and cyclooxygenase (COX)-2 expression including\n genes expression of cytokines were elevated by the six A.\n pasteurianus treatment. These results suggested that the six\n strains of A. pasteurianus have an excellent industrial\n application value as a functional material for the purpose of enhancing immune\n function.\n","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunomodulatory effects of six Acetobacter\\n pasteurianus strains in RAW-Blue macrophage\",\"authors\":\"S. Kim, W. Jeong, So-Young Kim, S. Yeo\",\"doi\":\"10.11002/kjfp.2023.30.1.65\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n \\n In this study, we investigated the immunological properties of six strains of\\n Acetobacter pasteurianus through nuclear factor-kappa\\n B/activator protein-1 (NF-κB/AP-1) transcription factor activation and\\n nitric oxide (NO) and cytokine production in macrophages. We found that the six\\n A. pasteurianus strains had no significant inhibitory\\n effect on the cell viability of RAW-Blue™ cells at the concentration of\\n (25, 50, 100 CFU/macrophage). The production of NO and cytokines (TNF-α,\\n IL-1β, and IL-6) showed different abilities of immune activation for each\\n strain, and it was 0.7 to 0.9 times higher than that of the LPS (100 ng/mL, v/v)\\n positive control and 7 to 8 times superior to that of the negative control\\n group. To explore the underlying mechanism, we evaluated the mRNA expression of\\n pro-inflammatory genes. Consequently, we found that inducible nitric oxide\\n synthase (iNOS) and cyclooxygenase (COX)-2 expression including\\n genes expression of cytokines were elevated by the six A.\\n pasteurianus treatment. These results suggested that the six\\n strains of A. pasteurianus have an excellent industrial\\n application value as a functional material for the purpose of enhancing immune\\n function.\\n\",\"PeriodicalId\":17875,\"journal\":{\"name\":\"Korean Journal of Food Preservation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Korean Journal of Food Preservation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11002/kjfp.2023.30.1.65\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Food Preservation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11002/kjfp.2023.30.1.65","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Immunomodulatory effects of six Acetobacter
pasteurianus strains in RAW-Blue macrophage
In this study, we investigated the immunological properties of six strains of
Acetobacter pasteurianus through nuclear factor-kappa
B/activator protein-1 (NF-κB/AP-1) transcription factor activation and
nitric oxide (NO) and cytokine production in macrophages. We found that the six
A. pasteurianus strains had no significant inhibitory
effect on the cell viability of RAW-Blue™ cells at the concentration of
(25, 50, 100 CFU/macrophage). The production of NO and cytokines (TNF-α,
IL-1β, and IL-6) showed different abilities of immune activation for each
strain, and it was 0.7 to 0.9 times higher than that of the LPS (100 ng/mL, v/v)
positive control and 7 to 8 times superior to that of the negative control
group. To explore the underlying mechanism, we evaluated the mRNA expression of
pro-inflammatory genes. Consequently, we found that inducible nitric oxide
synthase (iNOS) and cyclooxygenase (COX)-2 expression including
genes expression of cytokines were elevated by the six A.
pasteurianus treatment. These results suggested that the six
strains of A. pasteurianus have an excellent industrial
application value as a functional material for the purpose of enhancing immune
function.
期刊介绍:
This journal aims to promote and encourage the advancement of quantitative improvement for the storage, processing and distribution of food and its related disciplines, theory and research on its application. Topics covered include: Food Preservation and Packaging Food and Food Material distribution Fresh-cut Food Manufacturing Food processing Technology Food Functional Properties Food Quality / Safety.