强迫症患者巯基/二硫化物稳态和DNA氧化损伤的评估

IF 2.4 4区 医学 Q3 NEUROSCIENCES
Faruk Kurhan, H. Alp, M. Işık, Yavuz Selim Atan
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引用次数: 3

摘要

目的本研究旨在检测强迫症患者的硫醇/二硫化物稳态和DNA氧化损伤,并与健康对照组进行比较。方法纳入范大学精神医学系35例强迫症患者和33名健康志愿者。症状的严重程度采用耶鲁-布朗强迫量表进行测量。从患者组和对照组抽取5μL血样。在使用之前,将样品储存在适当的条件下。分离白细胞DNA,检测8-羟基-2-脱氧鸟苷(8-OHdG)和脱氧鸟苷的水平以评估DNA的氧化损伤。氧化性DNA损伤的水平表达为8-OHdG/106dG。测量硫醇/二硫化物稳态的总硫醇/天然硫醇水平。二硫化物的含量是通过从总硫醇值中减去天然硫醇值来确定的,结果除以2。结果以百分比表示。结果强迫症患者的总硫醇和天然硫醇水平显著低于健康对照组,二硫化物水平显著高于健康对照组。此外,与患者组相比,对照组的8-OHdG(DNA损伤的指标)显著降低。结论OCD患者二硫化物/天然硫醇和二硫化物/总硫醇水平的升高表明氧化应激水平升高,因此OCD患者较高的8-OHdG水平是DNA氧化损伤的标志。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Evaluation of Thiol/Disulfide Homeostasis and Oxidative DNA Damage in Patients with Obsessive Compulsive Disorder
Objective In this study, we aimed to examine thiol/disulfide homeostasis and oxidative DNA damage in patients with OCD and compare them with healthy controls. Methods Thirty-five patients previously diagnosed with OCD in Van Yuzuncu Yil University Department of Psychiatry and thirty-three healthy volunteers were included in the study. The severity of the symptoms was measured using the Yale-Brown Obsessive-Compulsive Scale. Five μL of blood samples were taken from the patient and control groups. The samples were stored at appropriate conditions until use. Leukocyte DNA was isolated and the levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) and deoxyguanosine were detected to assess the oxidative DNA damage. The level of oxidative DNA damage was expressed as 8-OHdG/106dG. Total thiol/native thiol levels were measured for thiol/disulfide homeostasis. The level of disulfide was determined by subtracting the native thiol value from the total thiol value and the result was divided by two. Results were given as percentages. Results The total and native thiol levels in patients with OCD were significantly lower, and the disulfide levels were significantly higher in patients with OCD than healthy control subjects. In addition, 8-OHdG, an indicator of DNA damage, was significantly lower in the control group compared to the patient group. Conclusion Increased levels of disulfide/native thiol and disulfide/total thiol in patients with OCD show that levels of oxidative stress were elevated and therefore, higher 8-OHdG levels in patients with OCD is a marker of oxidative DNA damage.
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来源期刊
Clinical Psychopharmacology and Neuroscience
Clinical Psychopharmacology and Neuroscience NEUROSCIENCESPHARMACOLOGY & PHARMACY-PHARMACOLOGY & PHARMACY
CiteScore
4.70
自引率
12.50%
发文量
81
期刊介绍: Clinical Psychopharmacology and Neuroscience (Clin Psychopharmacol Neurosci) launched in 2003, is the official journal of The Korean College of Neuropsychopharmacology (KCNP), and the associate journal for Asian College of Neuropsychopharmacology (AsCNP). This journal aims to publish evidence-based, scientifically written articles related to clinical and preclinical studies in the field of psychopharmacology and neuroscience. This journal intends to foster and encourage communications between psychiatrist, neuroscientist and all related experts in Asia as well as worldwide. It is published four times a year at the last day of February, May, August, and November.
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