O. Dubrovna, G. Priadkina, S. Mykhalska, A. Komisarenko
{"title":"含异源鸟氨酸-δ-氨基转移酶基因的转基因普通小麦Zymoyarka的耐水性","authors":"O. Dubrovna, G. Priadkina, S. Mykhalska, A. Komisarenko","doi":"10.15407/agrisp8.01.014","DOIUrl":null,"url":null,"abstract":"Aim. To determine water deficiency tolerance of genetically modified common wheat plants (Triticum aestivum L., cv\nZymoyarka), containing the heterologous ornithine-δ-aminotransferase gene, based on the analysis of grain productivity\nand physiological and biochemical characteristics in transgenic and non-transgenic genotypes. Methods. Biochemical\nspectrophotometric assays: the enzyme ornithine-δ-aminotransferase activity, the free L-proline content, and the\nphotosynthetic pigments content; biotechnological: Agrobacterium-mediated transformation in planta; physiological:\nmorphometric traits and elements of grain productivity; mathematical statistics. Results. It was established that the presence\nof an additional copy of the ornithine-δ-aminotransferase gene in transgenic plants leads to higher activity of the ornithine-δ-\naminotransferase enzyme: by 1.6 times higher on average for all lines as compared to the non-transgenic plants at 70 % of fi eld\ncapacity and by 1.5 – at 30 % fi eld capacity. However, transgenic plants did not differ significantly from the original variety\nin the free L-proline content either under optimal water conditions or under soil drought. The increase in the total chlorophyll\n(a + b) content in flag leaves of transgenic plants was established under conditions of both optimal water supply and drought,\nas compared with the original genotype (increase by 5–7 % and 8–11 %, respectively). The enhanced expression of the orni-\nthine-δ-aminotransferase gene in the transgenic plants stimulated root growth both under optimal and stressful conditions:\nthe root length of the transformed plants exceeded that of the original variety by 3.4–3.9 cm in the variant with optimal\nwater supply, and by 4.2–4.6 cm – under drought. They were also characterized by a more developed root system. Dry root\nweight of the transgenic plants exceeded the original variety both in the control (by 23–27 %), and under drought (by 37–\n44 %). Under drought, the root dry weight decreased by 29 % in the plants of the original variety, compared 70 % fi eld\ncapacity, and by 11–15 % in the lines. Under 30 % field capacity, the transgenic lines also exceeded non-transformed plants\nin the number of grains from the whole plant (on average for 3 lines by 26 %) and in the grain weight (by 22 %). Transgenic\nplants are characterized by the formation of a higher productive shoots number: from 3.2 to 3.4 compared with 2.5 in\nnon-transgenic plants at 70 % fi eld capacity and 2.7–3.1 vs 2.2 at 30 % field capacity it was found. Conclusions. Thus,\nthe analysis of genetically modified common wheat plants cv. Zymoyarka, containing the heterologous alfalfa ornithine-δ-\naminotransferase gene, by yield structure elements, morphometric parameters and photosynthetic pigment content showed\ntheir better tolerance to soil drought as compared to non-transgenic plants. We explain the improvement of grain productivity\nof the whole plant in transgenic wheat lines with an additional copy of ornithine-δ-aminotransferase gene by the fact that\nthey have a better developed root system (dry root weight of the transgenic plants exceeded the original variety both in the\ncontrol by 23–27 %, and under drought by 37–44 %) and a higher (on average for 3 lines – 3.3 compared to 2.5 in non-\ntransgenic plants at 70 % fi eld capacity and 2.9 vs 2.2 at 30 % fi eld capacity) number of productive shoots than in the origi-\nnal variety both under optimal and insuffi cient water supply.","PeriodicalId":55933,"journal":{"name":"Agricultural Science and Practice","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2021-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Water deficiency tolerance of genetically modified common wheat cv. Zymoyarka, containing a heterologous ornithine-δ-aminotransferase gene\",\"authors\":\"O. Dubrovna, G. Priadkina, S. Mykhalska, A. Komisarenko\",\"doi\":\"10.15407/agrisp8.01.014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aim. To determine water deficiency tolerance of genetically modified common wheat plants (Triticum aestivum L., cv\\nZymoyarka), containing the heterologous ornithine-δ-aminotransferase gene, based on the analysis of grain productivity\\nand physiological and biochemical characteristics in transgenic and non-transgenic genotypes. Methods. Biochemical\\nspectrophotometric assays: the enzyme ornithine-δ-aminotransferase activity, the free L-proline content, and the\\nphotosynthetic pigments content; biotechnological: Agrobacterium-mediated transformation in planta; physiological:\\nmorphometric traits and elements of grain productivity; mathematical statistics. Results. It was established that the presence\\nof an additional copy of the ornithine-δ-aminotransferase gene in transgenic plants leads to higher activity of the ornithine-δ-\\naminotransferase enzyme: by 1.6 times higher on average for all lines as compared to the non-transgenic plants at 70 % of fi eld\\ncapacity and by 1.5 – at 30 % fi eld capacity. However, transgenic plants did not differ significantly from the original variety\\nin the free L-proline content either under optimal water conditions or under soil drought. The increase in the total chlorophyll\\n(a + b) content in flag leaves of transgenic plants was established under conditions of both optimal water supply and drought,\\nas compared with the original genotype (increase by 5–7 % and 8–11 %, respectively). The enhanced expression of the orni-\\nthine-δ-aminotransferase gene in the transgenic plants stimulated root growth both under optimal and stressful conditions:\\nthe root length of the transformed plants exceeded that of the original variety by 3.4–3.9 cm in the variant with optimal\\nwater supply, and by 4.2–4.6 cm – under drought. They were also characterized by a more developed root system. Dry root\\nweight of the transgenic plants exceeded the original variety both in the control (by 23–27 %), and under drought (by 37–\\n44 %). Under drought, the root dry weight decreased by 29 % in the plants of the original variety, compared 70 % fi eld\\ncapacity, and by 11–15 % in the lines. Under 30 % field capacity, the transgenic lines also exceeded non-transformed plants\\nin the number of grains from the whole plant (on average for 3 lines by 26 %) and in the grain weight (by 22 %). Transgenic\\nplants are characterized by the formation of a higher productive shoots number: from 3.2 to 3.4 compared with 2.5 in\\nnon-transgenic plants at 70 % fi eld capacity and 2.7–3.1 vs 2.2 at 30 % field capacity it was found. Conclusions. Thus,\\nthe analysis of genetically modified common wheat plants cv. Zymoyarka, containing the heterologous alfalfa ornithine-δ-\\naminotransferase gene, by yield structure elements, morphometric parameters and photosynthetic pigment content showed\\ntheir better tolerance to soil drought as compared to non-transgenic plants. We explain the improvement of grain productivity\\nof the whole plant in transgenic wheat lines with an additional copy of ornithine-δ-aminotransferase gene by the fact that\\nthey have a better developed root system (dry root weight of the transgenic plants exceeded the original variety both in the\\ncontrol by 23–27 %, and under drought by 37–44 %) and a higher (on average for 3 lines – 3.3 compared to 2.5 in non-\\ntransgenic plants at 70 % fi eld capacity and 2.9 vs 2.2 at 30 % fi eld capacity) number of productive shoots than in the origi-\\nnal variety both under optimal and insuffi cient water supply.\",\"PeriodicalId\":55933,\"journal\":{\"name\":\"Agricultural Science and Practice\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2021-06-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural Science and Practice\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15407/agrisp8.01.014\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Science and Practice","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15407/agrisp8.01.014","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
Water deficiency tolerance of genetically modified common wheat cv. Zymoyarka, containing a heterologous ornithine-δ-aminotransferase gene
Aim. To determine water deficiency tolerance of genetically modified common wheat plants (Triticum aestivum L., cv
Zymoyarka), containing the heterologous ornithine-δ-aminotransferase gene, based on the analysis of grain productivity
and physiological and biochemical characteristics in transgenic and non-transgenic genotypes. Methods. Biochemical
spectrophotometric assays: the enzyme ornithine-δ-aminotransferase activity, the free L-proline content, and the
photosynthetic pigments content; biotechnological: Agrobacterium-mediated transformation in planta; physiological:
morphometric traits and elements of grain productivity; mathematical statistics. Results. It was established that the presence
of an additional copy of the ornithine-δ-aminotransferase gene in transgenic plants leads to higher activity of the ornithine-δ-
aminotransferase enzyme: by 1.6 times higher on average for all lines as compared to the non-transgenic plants at 70 % of fi eld
capacity and by 1.5 – at 30 % fi eld capacity. However, transgenic plants did not differ significantly from the original variety
in the free L-proline content either under optimal water conditions or under soil drought. The increase in the total chlorophyll
(a + b) content in flag leaves of transgenic plants was established under conditions of both optimal water supply and drought,
as compared with the original genotype (increase by 5–7 % and 8–11 %, respectively). The enhanced expression of the orni-
thine-δ-aminotransferase gene in the transgenic plants stimulated root growth both under optimal and stressful conditions:
the root length of the transformed plants exceeded that of the original variety by 3.4–3.9 cm in the variant with optimal
water supply, and by 4.2–4.6 cm – under drought. They were also characterized by a more developed root system. Dry root
weight of the transgenic plants exceeded the original variety both in the control (by 23–27 %), and under drought (by 37–
44 %). Under drought, the root dry weight decreased by 29 % in the plants of the original variety, compared 70 % fi eld
capacity, and by 11–15 % in the lines. Under 30 % field capacity, the transgenic lines also exceeded non-transformed plants
in the number of grains from the whole plant (on average for 3 lines by 26 %) and in the grain weight (by 22 %). Transgenic
plants are characterized by the formation of a higher productive shoots number: from 3.2 to 3.4 compared with 2.5 in
non-transgenic plants at 70 % fi eld capacity and 2.7–3.1 vs 2.2 at 30 % field capacity it was found. Conclusions. Thus,
the analysis of genetically modified common wheat plants cv. Zymoyarka, containing the heterologous alfalfa ornithine-δ-
aminotransferase gene, by yield structure elements, morphometric parameters and photosynthetic pigment content showed
their better tolerance to soil drought as compared to non-transgenic plants. We explain the improvement of grain productivity
of the whole plant in transgenic wheat lines with an additional copy of ornithine-δ-aminotransferase gene by the fact that
they have a better developed root system (dry root weight of the transgenic plants exceeded the original variety both in the
control by 23–27 %, and under drought by 37–44 %) and a higher (on average for 3 lines – 3.3 compared to 2.5 in non-
transgenic plants at 70 % fi eld capacity and 2.9 vs 2.2 at 30 % fi eld capacity) number of productive shoots than in the origi-
nal variety both under optimal and insuffi cient water supply.