烟台市2018-2021年与手足口病相关的柯萨奇病毒A16流行病学及遗传特征

IF 3.5 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Zhenlu Sun , Ji Wang , Xin Chi , Peihua Niu , Ruiqing Zhang , Qiao Gao , Yong Zhang , Xuejun Ma
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引用次数: 0

摘要

2008年,中国启动了全国手足口病监测系统。烟台市每年报告数百万例手足口病,柯萨奇病毒A16 (CVA16)是近年来造成烟台市手足口病流行的主要原因,但对烟台市CVA16的流行病学和分子特征的了解有限。本研究旨在了解烟台市2018 - 2021年手足口病的流行病学特征和致病谱,以及CVA16的分子特征。在烟台市采集2018 - 2021年临床样本2000份,采用实时逆转录-聚合酶链反应(qRT-PCR)进行肠道病毒分型。对41株CVA16分离株的VP1编码区进行扩增和Sanger测序,并进行系统发育分析。在研究期间,手足口病的流行时间为每年5 - 8月。它在6月达到顶峰,在9月下降。发病率在1至5岁儿童中最高,而男性比女性更常见。在2000个临床收集的样本中,有1617个被检测为肠道病毒阳性。其中CVA16型614例,A71型肠病毒(EV A17) 45例,其他血清型958例。41株CVA16均为Bla和B1b基因型。同源性分析表明,41株CVA16菌株之间核苷酸相似性为83.2% ~ 100%,氨基酸相似性为93.7% ~ 100%。本研究结果更新了CVA16的分子流行病学,为手足口病的防治提供参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Epidemiology and genetic characteristics of coxsackievirus A16 associated with hand-foot-and-mouth disease in Yantai city, China in 2018–2021

In 2008, China launched a national surveillance system for hand-foot-and-mouth disease (HFMD). Several million cases of HFMD are reported every year, coxsackievirus A16 (CVA16) was the leading cause of HFMD epidemic in Yantai city, China in recent years, but the information of epidemiology and molecular characterization of CVA16 in Yantai is limited. The aim of this study is to investigate the epidemiological characteristics and pathogenic spectrum of HFMD, and most importantly, the molecular characterization of CVA16 in Yantai from 2018 to 2021. A total of 2,000 clinical samples were collected in Yantai city from 2018 to 2021 and the enterovirus typing was performed using real-time reverse transcriptase–polymerase chain reaction (qRT-PCR). VP1 coding regions of 41 CVA16 isolates were amplified and Sanger sequenced, and phylogenetic analysis was performed. During the study period, HFMD became prevalent from May to August each year. It peaked in June and declined in September. The incidence was highest in children aged 1 to 5 years, while more common in males than females. 1,617 out of 2,000 clinical collection of samples were tested positive for enterovirus. Among them, 614 were identified as CVA16, 45 were enterovirus A71 (EV A17), and 958 were other enterovirus serotypes. All 41 CVA16 strains belonged to the Bla and B1b genotypes. Homology analysis showed that 41 CVA16 isolates shared 83.2%–100% nucleotide and 93.7%–100% amino acid similarity among themselves. The results of this study update molecular epidemiology of CVA16 and provide a reference for HFMD prevention and control.

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来源期刊
Biosafety and Health
Biosafety and Health Medicine-Infectious Diseases
CiteScore
7.60
自引率
0.00%
发文量
116
审稿时长
66 days
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