IGF-I基因在肝脏和白肌肉中的表达证实了日粮NaCl对巨鲟幼鱼生长指数的促进作用

IF 0.8 4区农林科学 Q3 FISHERIES

Iranian Journal of Fisheries Sciences Pub Date : 2020-09-10 DOI:10.22092/IJFS.2020.122567

Somayeh Hasanpoor, M. G. Marzdashti, H. V. Roodsari, R. Kazemi, Seyed Hamed Mosavi sabet

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引用次数: 1

摘要

本研究基于胰岛素样生长因子（IGF）、IGF mRNA在肝脏和肌肉中的基因表达，评估了不同水平的NaCl在培养的Huso Huso幼鱼饮食中的作用。共有480只平均体重173.8±0.49g的幼年白鲸标本，存放在12个水槽中，饲养12周。NaCl以4个水平添加，并以0（对照）、3%、6%和9%的三个重复添加到基础日粮中。12周后，从16个肝脏和肌肉中提取总RNA，从两个重复的处理中取样，并通过电泳进行评估。β2m-qPCR引物作为基因参考。使用琼脂糖凝胶（1.5%）和基因测序确认从引物获得的产物的大小和引物的特定作用程度。结果显示，治疗组和对照组IGF mRNA表达发生显著变化（p<0.05）。IGF基因表达数据显示，对照组和治疗组IGF基因水平最高，分别为3%。此外，在白肌组织中，IGF mRNA基因表达在对照组中最高（6.4），在治疗组中最低（0.7）和治疗组中为6%（0.8），肝体细胞指数（his）和平均日生长量（ADG）在所有治疗中均显著增加，治疗组和对照组之间有显著差异（p<0.05）。对照组的SGR从1.41增加到治疗组的1.58，治疗3%和6%，BWI从对照组的68.88增加到治疗6%的98.28。HSI从对照组的3.64g增加到治疗组的4.37g。FCR在对照组中显示出显著差异（1.27），在3%和9%中分别为1.14。对FCR、SGR和IGF mRNA基因表达的统计分析显示，含NaCl饮食喂养的鱼类生长指数更好。

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IGF-I gene expression in liver and white muscles confirming promotion effect of dietary NaCl on Growth indices of Giant sturgeon (Huso huso) juveniles

TThis study was conducted to evaluate effects of different levels of NaCl used in the diet of cultivated Huso huso juveniles based on gene expression of Insulin-like Growth Factor (IGF), IGF mRNA in the liver and muscle. Totally 480 specimen beluga juvenile by the average weight of 173.8±0.49 g stocked in 12 tanks and reared for 12 weeks. The NaCl added in 4 levels and with three replicates of 0(control), 3, 6 and 9 percent to the basic diet. After 12 weeks total RNA extracted from the16 liver and muscle which were sampled from two replicates of treatments and evaluated by electrophoresis. β2m-qPCR primer was used as gene reference. The size of the product obtained from primers and the specific degree of action of primers was confirmed using agarose gel (1.5%) and the sequencing of the genes.According to the results, significant changes in IGF mRNA expression observed in the treatments and control group (p <0.05). The data of IGF gene expressions showed that the highest levels observed in the control group and treatment 3% respectively. Moreover, in the tissue of white muscle, IGF mRNA gene expression showed highest gene expression in the control group (6.4) and lowest expression were in treatment 3% (0.7) and treatment 6% (0.8). Also, growth indices results including body weight, Specific Growth Rate(SGR), Body Weight Index(BWI), Hepatosomatic Index(his) and Average Daily Growth(ADG) significantly were increased in all treatments, with significant differences among treatments and the control group (p <0.05). The SGR in the control group increased from 1.41 to 1.58 in treatments 3% and 6% and the BWI increased from 68.88 in the control group to 98.28 in treatment 6%. HSI increased from 3.64 g in the control group to 4.37 g in treatment two. FCR showed a significant difference in the control group (1.27) and 1.14 in 3% and 9%, respectively. Statistical analysis of FCR, SGR and IGF mRNA gene expression showed better growth indices in fish fed by a diet containing NaCl.

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来源期刊

Iranian Journal of Fisheries Sciences FISHERIES-

CiteScore

2.30

自引率

11.10%

发文量

审稿时长

3 months

期刊介绍： 1- Living various species (contains animals and vegetal species) in various aquatic ecosystems. 2- Health and diseases of aquatic species. 3- Determining the stocks and specific time and location for catching and reliable exploitation for sustainable development. 4- Methods of propagation and culture of high value aquatic resources. 5- Aquatic stock assessment and the methods of restocking the high value species and suggestion for rate, areas and the time for releasing fish and other aquatic organisms fries. 6- Pollutant agents and their effects to the environments of aquatic species. 7- Feed and feeding in aquatic organisms. 8- Fish processing and producing new products. 9- The economic and social aspects of fisheries.