卡纳塔克邦特有植物Curcuma karnatakensis直接和愈伤组织介导的高效离体再生方法

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2021-07-03 DOI:10.1080/10496475.2020.1806166

Annapurna Annayappa Shanthala, Abhirami Dilkalal, T. Umesh

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引用次数: 2

摘要

摘要建立了一种直接和愈伤组织介导的姜黄全株再生的体外实验方案。以根状茎芽为外植体，在添加2mgL-16-苄基腺嘌呤（BA）的MS培养基上培养，平均每个外植体可产生4.00±0.57芽。愈伤组织诱导和增殖研究表明，在添加2mgL−1 2,4-二氯苯氧乙酸的MS培养基上，根茎外植体（68%的外植体在接种后30d内诱导出愈伤组织）的愈伤组织诱导率高于茎基外植体（54%的外植体，60d内诱导）。利用根状茎来源的愈伤组织在高浓度α-萘乙酸（5 mgL−1）的培养基上诱导器官源性愈伤组织，并将其转移到含有0.5 mgL−1NAA和4mgL−1BA的再生培养基上，培养8周后每个外植体产生5.33±0.66芽。体外再生植物成功适应温室条件，存活率为85%，表现出正常表型。

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An Efficient Invitro Approach for Direct and Callus Mediated Regeneration of Curcuma karnatakensis – An Endemic Plant of Karnataka

ABSTRACT An in vitro protocol for direct and callus mediated regeneration of complete plantlets of Curcuma karnatakensis was established. For direct regeneration rhizome buds were used as explants and culturing them on MS medium supplemented with 2 mgL−1 6-Benzyladenine (BA) resulted with an average of 4.00 ± 0.57 shoots per explant. Callus induction and multiplication study revealed that the percentage of callus induction was better in rhizome explants (68% explants induced callus within 30 d of inoculation) compared to shoot base explants (54% explants induced in 60 d) on MS medium supplemented with 2 mgL−1 2,4-Dichlorophenoxyacetic acid. Rhizome derived calli were used to induce organogenic callus on medium with higher concentration of α-Naphthalene acetic acid (5 mgL−1) and further transferred to regeneration medium containing 0.5 mgL−1 NAA and 4mgL−1 BA which produced 5.33 ± 0.66 shoots per explant in 8 weeks of culture. In vitro regenerated plants were successfully acclimatized to greenhouse conditions with a survival rate of 85% and exhibited normal phenotypes.

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.