马马菌内生真菌抑菌活性测定及菌株R1的化学性质研究

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2019-08-07 DOI:10.3329/BJP.V14I3.41598

Xuelian Bai, Ronglu Yu, Mingzhu Li, Huawei Zhang

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引用次数: 6

摘要

为了寻找更多的抗微生物天然产物用于新药开发，对印度酸模内生菌株进行了生物测定和化学研究。成功分离到6株真菌（编号为L1、L6、L9、L10、R1、R2），并评价了它们对大肠杆菌、金黄色葡萄球菌和白色念珠菌三种人类病原体的抗菌效果。结果表明，这些菌株发酵液的乙酸乙酯提取物对大肠杆菌和白色念珠菌没有很强的抗微生物活性，而L1和R1菌株对金黄色葡萄球菌有较强的抑制作用。根据形态特征和18S rRNA序列分析，将菌株L1和R1分别归类为Colletotrichum和Fusarium geni。对菌株R1的化学研究分离出两种已知的抗菌剂，邻苯二甲酸二丁酯（1）和白僵菌素（2）。

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Antimicrobial assay of endophytic fungi from Rumex madaio and chemical study of strain R1

In order to search more antimicrobial natural products for new drug discovery, bioassay and chemical investigation of endophytic strains from Rumex madaio were carried out. Six fungal strains (numbered as L1, L6, L9, L10, R1, R2) were successfully isolated and their antimicrobial effects on three human pathogens, Escherichia coli, Staphyloccocus aureus and Candida albicans, were evaluated. The results indicated that ethyl acetate extracts of fermentation broth of these strains had no potent antimicorbial activity against E. coli and C. albicans while strains L1 and R1 had stronger inhibitory effects on S. aureus. On basis of morphological characteristics and 18S rRNA sequence analysis, strains L1 and R1 were respectively classified as Colletotrichum and Fusarium geni. The chemical study of strain R1 led to isolation of two known antimicrobial agents, dibutyl phthalate (1) and beauvericin (2).

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.