从青贮饲料中分离的乳酸菌从农工废弃物中生产胞外多糖

IF 0.7 Q4 PHARMACOLOGY & PHARMACY
Ahmed R Henawy, A. Abdelhadi, Asmaa Halema, Refae Refae, Olfat Barakat
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引用次数: 0

摘要

背景和目的乳酸菌(L.A.B.)可以利用农业和工业废料生产胞外多糖。这种方法除了可以生产有价值的产品外,还可以防止这些废物在环境中的有害处理和堆积。选择13株产乳酸菌的EPS分离株,通过Rep-PCR-DNA指纹图谱测定它们之间的相似性和距离指数,并从青贮饲料样品中对乳酸菌进行分子鉴定。除了优化来自可再生资源的多糖外,还对分离菌株产生胞外多糖的能力进行了评估。材料和方法通过16S rRNA基因测序对产生LAB的EPS分离株进行分子鉴定,并将其DNA序列保存到NCBI中。使用检测的13株菌株在MRS作为标准生产培养基上进行EPS生产,范围在1.53至7.53之间 g/l。然后,从农业工业废物甘蔗糖蜜、腌奶酪乳清及其混合物中进一步检测了产生EPS的最高显著菌株,即副干酪乳酸杆菌菌株LAB 64、鼠李糖乳酸杆菌菌株LAB 160和鼠李糖乳杆菌菌株LAB192。结果与结论三株菌株在糖蜜与乳清(1/1 v/v)。在几乎所有情况下,向生产混合物中添加碳酸钙都显著提高了EPS的产量,并且中和介质很重要。此外,将发酵混合物的混合糖浓度从2%提高到5%,提高了所有菌株的EPS产量。在这方面,乳酸菌的EPS产量增加了2倍。鼠李糖菌株LAB 160 22.39 g/l。与EPS标准品相比,使用FT-IR和HPLC对EPS产物进行提取和分析。FTIR和HPLC分析证实该聚合物为α-葡聚糖,通过将其保留时间与葡聚糖标准的保留时间进行比较,确定其为葡聚糖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exopolysaccharide production from agro-industrial wastes by lactic acid bacteria isolated from silage
Background and Objectives Lactic acid bacteria (L.A.B.) can produce exopolysaccharides (EPSs) using agricultural and industrial waste materials. This approach can prevent the harmful disposal and buildup of these wastes in the environment in addition to producing valuable products. Thirteen LAB-producing EPS isolates were selected, and the similarity and distance indices were determined between them through Rep-PCR DNA fingerprinting, and molecularly identified LAB from silage samples. Evaluation of the ability of the isolated strains to produce exopolysaccharides was carried out, in addition to the optimization of the polysaccharides from renewable resources. Materials and methods LAB-producing EPS isolates were molecularly identified by the 16S rRNA gene sequencing and deposited their DNA sequences to NCBI. EPS production using the examined 13 strains was carried out on MRS as a standard production medium and ranged between 1.53 and 7.53 g/l. Then, the highest significant EPS-producing strains i.e., Lacticaseibacillus paracasei strain LAB 64, Lacticaseibacillus rhamnosus strain LAB 160, and Lacticaseibacillus rhamnosus strain LAB 192 were further examined for EPS production from the agro-industrial wastes sugarcane molasses, salted cheese whey, and their mixture. Results and conclusion The maximum EPS production by the three strains was obtained in a mixture of molasses: whey (1/1 v/v). Calcium carbonate addition to the production mixture significantly improved EPS production in almost all cases and it is important to neutralize the media. Moreover, increasing the mixture sugar concentration of the fermentation mixture from 2% to 5% enhanced EPS production by all strains. In this regard, a 2-fold increment in EPS production was achieved by the Lactic. rhamnosus strain LAB 160 22.39 g/l. The extraction and analysis of the EPS product were carried out using both FT-IR and HPLC compared to an EPS standard. FTIR and HPLC analysis confirmed the polymer as an α-glucan, which was identified as dextran through a comparison between its retention time and the retention time of the dextran standard.
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来源期刊
Egyptian Pharmaceutical Journal
Egyptian Pharmaceutical Journal PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
0.00%
发文量
37
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