V I Seledtsov, V V Malashchenko, M E Meniailo, N D Gazatova, G V Seledtsova
{"title":"粒细胞集落刺激因子在活化的人巨噬细胞中下调干扰素受体的表达并刺激白细胞介素-6的产生","authors":"V I Seledtsov, V V Malashchenko, M E Meniailo, N D Gazatova, G V Seledtsova","doi":"10.1080/08977194.2019.1662418","DOIUrl":null,"url":null,"abstract":"<p><p>We studied direct effects of human granulocyte colony-stimulating factor (G-CSF) on phenotypical properties of human macrophage cells <i>in vitro</i>. CD14<sup>+</sup> monocyte/macrophages (Mc/Mphs) were isolated from blood of healthy donors by positive magnetic separation. G-CSF (0.01-1.0 ng/mL), when added to Mc/Mphs along with lipopolysaccharide (LPS, 1.0 μg/mL), was able to noticeably reduce proportions of CD119 (interferon-γ receptor 1)-positive cells, with no stable effects on CD16 (FcγRIII)<sup>+</sup> and СD124 (IL-4 receptor subunit alpha)-positive cells. In addition, G-CSF markedly upregulated IL-6 production by LPS-activated Mph cells, without significantly affecting IL-1β, IL-10 and tumor necrosis factor-α (TNF-α) secretion. Our data suggests that G-CSF could restrain Mph polarization to pro-inflammatory (M1) phenotype, thus potentially supporting pro-regenerative Mph activity with implications for immunotherapeutic interventions.</p>","PeriodicalId":12782,"journal":{"name":"Growth factors","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08977194.2019.1662418","citationCount":"1","resultStr":"{\"title\":\"Granulocyte colony-stimulating factor downregulates interferon-gamma receptor expression and stimulates interleukin-6 production in activated human macrophages.\",\"authors\":\"V I Seledtsov, V V Malashchenko, M E Meniailo, N D Gazatova, G V Seledtsova\",\"doi\":\"10.1080/08977194.2019.1662418\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We studied direct effects of human granulocyte colony-stimulating factor (G-CSF) on phenotypical properties of human macrophage cells <i>in vitro</i>. CD14<sup>+</sup> monocyte/macrophages (Mc/Mphs) were isolated from blood of healthy donors by positive magnetic separation. G-CSF (0.01-1.0 ng/mL), when added to Mc/Mphs along with lipopolysaccharide (LPS, 1.0 μg/mL), was able to noticeably reduce proportions of CD119 (interferon-γ receptor 1)-positive cells, with no stable effects on CD16 (FcγRIII)<sup>+</sup> and СD124 (IL-4 receptor subunit alpha)-positive cells. In addition, G-CSF markedly upregulated IL-6 production by LPS-activated Mph cells, without significantly affecting IL-1β, IL-10 and tumor necrosis factor-α (TNF-α) secretion. Our data suggests that G-CSF could restrain Mph polarization to pro-inflammatory (M1) phenotype, thus potentially supporting pro-regenerative Mph activity with implications for immunotherapeutic interventions.</p>\",\"PeriodicalId\":12782,\"journal\":{\"name\":\"Growth factors\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2019-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/08977194.2019.1662418\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Growth factors\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/08977194.2019.1662418\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2019/9/18 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Growth factors","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/08977194.2019.1662418","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2019/9/18 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
Granulocyte colony-stimulating factor downregulates interferon-gamma receptor expression and stimulates interleukin-6 production in activated human macrophages.
We studied direct effects of human granulocyte colony-stimulating factor (G-CSF) on phenotypical properties of human macrophage cells in vitro. CD14+ monocyte/macrophages (Mc/Mphs) were isolated from blood of healthy donors by positive magnetic separation. G-CSF (0.01-1.0 ng/mL), when added to Mc/Mphs along with lipopolysaccharide (LPS, 1.0 μg/mL), was able to noticeably reduce proportions of CD119 (interferon-γ receptor 1)-positive cells, with no stable effects on CD16 (FcγRIII)+ and СD124 (IL-4 receptor subunit alpha)-positive cells. In addition, G-CSF markedly upregulated IL-6 production by LPS-activated Mph cells, without significantly affecting IL-1β, IL-10 and tumor necrosis factor-α (TNF-α) secretion. Our data suggests that G-CSF could restrain Mph polarization to pro-inflammatory (M1) phenotype, thus potentially supporting pro-regenerative Mph activity with implications for immunotherapeutic interventions.
期刊介绍：
Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications
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