{"title":"海南芦荟皂山楂多糖的抗炎活性研究","authors":"Jae‐Ran Kang, G. Kim, Jung-Hye Shin","doi":"10.11002/kjfp.2022.29.7.1189","DOIUrl":null,"url":null,"abstract":"\n \n To identify the physiological activity of Saponaria aloe cultivated in the\n Namhae-gun, polysaccharides, as the main physiologically active substances, were\n isolated and their antioxidant and anti-inflammatory activities were confirmed.\n Hot-air dried saponaria were extracted with hot water (80°C, 24 h) and\n then the extracts were freeze dried (HWE). HWE was dialyzed using 3.5 kDa\n dialysis membrane and only intra-dialysis solution was taken and freeze dried\n (IDS) for experiment. Total polysaccharide content was higher in IDS (195.32\n mg/g) than HWE however, anthraquinone content was lower in IDS. Total phenolic\n compounds (TPC) contents of HWE and IDS were 594.04 and 1,047.23 mg/100 g,\n respectively. The IC50 values of\n 2,2-Azinobis-(3-ethylbenzo-thiazoline-6-sulphonate (ABTS) and\n 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were higher\n 1.7 and 1.8 folds in HWE than IDS, respectively. The MTT assay showed that the\n sample concentrations below than 100 μg/mL were\n sufficient to exert anti-inflammatory activity in lipopolysaccharide (LPS)\n induced RAW 264.7 cells. The production levels of nitric oxide (NO) and reactive\n oxygen species (ROS) were significantly decreased in 100\n μg/mL of HWE (p⟨0.01) and IDS\n (p⟨0.001) treated group than those in LPS only treated group. The\n expression levels of tumor necrosis faction (TNF)-α and\n interleukin (IL)-6 were significantly lower in all treated samples than in the\n LPS only treated group. However, there was no significant difference between the\n HWE and IDS-treated group. The expression activity of\n IL-1β was no significant difference among all\n samples.\n","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Anti-inflammatory activities of polysaccharides isolated from\\n Aloe saponaria Haw grown in Namhae\",\"authors\":\"Jae‐Ran Kang, G. Kim, Jung-Hye Shin\",\"doi\":\"10.11002/kjfp.2022.29.7.1189\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n \\n To identify the physiological activity of Saponaria aloe cultivated in the\\n Namhae-gun, polysaccharides, as the main physiologically active substances, were\\n isolated and their antioxidant and anti-inflammatory activities were confirmed.\\n Hot-air dried saponaria were extracted with hot water (80°C, 24 h) and\\n then the extracts were freeze dried (HWE). HWE was dialyzed using 3.5 kDa\\n dialysis membrane and only intra-dialysis solution was taken and freeze dried\\n (IDS) for experiment. Total polysaccharide content was higher in IDS (195.32\\n mg/g) than HWE however, anthraquinone content was lower in IDS. Total phenolic\\n compounds (TPC) contents of HWE and IDS were 594.04 and 1,047.23 mg/100 g,\\n respectively. The IC50 values of\\n 2,2-Azinobis-(3-ethylbenzo-thiazoline-6-sulphonate (ABTS) and\\n 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were higher\\n 1.7 and 1.8 folds in HWE than IDS, respectively. The MTT assay showed that the\\n sample concentrations below than 100 μg/mL were\\n sufficient to exert anti-inflammatory activity in lipopolysaccharide (LPS)\\n induced RAW 264.7 cells. The production levels of nitric oxide (NO) and reactive\\n oxygen species (ROS) were significantly decreased in 100\\n μg/mL of HWE (p⟨0.01) and IDS\\n (p⟨0.001) treated group than those in LPS only treated group. The\\n expression levels of tumor necrosis faction (TNF)-α and\\n interleukin (IL)-6 were significantly lower in all treated samples than in the\\n LPS only treated group. However, there was no significant difference between the\\n HWE and IDS-treated group. The expression activity of\\n IL-1β was no significant difference among all\\n samples.\\n\",\"PeriodicalId\":17875,\"journal\":{\"name\":\"Korean Journal of Food Preservation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Korean Journal of Food Preservation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11002/kjfp.2022.29.7.1189\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Food Preservation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11002/kjfp.2022.29.7.1189","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Anti-inflammatory activities of polysaccharides isolated from
Aloe saponaria Haw grown in Namhae
To identify the physiological activity of Saponaria aloe cultivated in the
Namhae-gun, polysaccharides, as the main physiologically active substances, were
isolated and their antioxidant and anti-inflammatory activities were confirmed.
Hot-air dried saponaria were extracted with hot water (80°C, 24 h) and
then the extracts were freeze dried (HWE). HWE was dialyzed using 3.5 kDa
dialysis membrane and only intra-dialysis solution was taken and freeze dried
(IDS) for experiment. Total polysaccharide content was higher in IDS (195.32
mg/g) than HWE however, anthraquinone content was lower in IDS. Total phenolic
compounds (TPC) contents of HWE and IDS were 594.04 and 1,047.23 mg/100 g,
respectively. The IC50 values of
2,2-Azinobis-(3-ethylbenzo-thiazoline-6-sulphonate (ABTS) and
1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were higher
1.7 and 1.8 folds in HWE than IDS, respectively. The MTT assay showed that the
sample concentrations below than 100 μg/mL were
sufficient to exert anti-inflammatory activity in lipopolysaccharide (LPS)
induced RAW 264.7 cells. The production levels of nitric oxide (NO) and reactive
oxygen species (ROS) were significantly decreased in 100
μg/mL of HWE (p⟨0.01) and IDS
(p⟨0.001) treated group than those in LPS only treated group. The
expression levels of tumor necrosis faction (TNF)-α and
interleukin (IL)-6 were significantly lower in all treated samples than in the
LPS only treated group. However, there was no significant difference between the
HWE and IDS-treated group. The expression activity of
IL-1β was no significant difference among all
samples.
期刊介绍:
This journal aims to promote and encourage the advancement of quantitative improvement for the storage, processing and distribution of food and its related disciplines, theory and research on its application. Topics covered include: Food Preservation and Packaging Food and Food Material distribution Fresh-cut Food Manufacturing Food processing Technology Food Functional Properties Food Quality / Safety.