Jin-Hee Kim, Song-Yi Choi, Injun Hwang, Jeong-Eun Hyun, S. A. Kim, G. Ban, Se-Ri Kim
{"title":"双孢蘑菇生产中微生物安全性及交叉污染源评价","authors":"Jin-Hee Kim, Song-Yi Choi, Injun Hwang, Jeong-Eun Hyun, S. A. Kim, G. Ban, Se-Ri Kim","doi":"10.11002/kjfp.2022.29.7.1079","DOIUrl":null,"url":null,"abstract":"\n \n This study aimed to investigate the contamination sources of button mushrooms by\n assessing the microbial load on the mushrooms and in the agricultural inputs\n that come into contact with the mushroom. A total of 234 samples including\n mushrooms, compost, casing soil, and tools from A, B, C, and D farms were tested\n for sanitary indicators (aerobic bacteria, coliforms, and Escherichia\n coli) and foodborne pathogens (E. coli O157:H7,\n Salmonella spp., Staphylococcus aureus,\n and Listeria monocytogenes). To identify the contamination\n source, E. coli was isolated and analyzed using MLST\n (Multi-Locus Sequence Typing). The number of aerobic bacteria remained\n consistent during composting (⟩7 log CFU/g); however, the occurrence of\n coliforms and E. coli was reduced to the detection limit\n (⟨1.0 log). Salmonella spp. and L.\n monocytogenes were detected in the raw material; however, they were\n inactivated during composing. More than 6.0 log of aerobic bacteria and 3.0 log\n of coliforms were observed in the casing soil from A, B, and C farms and in the\n gloves from the C farm. MLST indicated that the STs of E. coli\n isolated from casing soil layer and from the harvested mushrooms were identical\n at ST 189. Therefore, cross contamination occurred through the casing soil\n layer, and this could contribute to E. coli contamination in\n mushrooms. It is necessary to practice hygiene guidelines, such as sterilization\n of casing soil, worker hygiene, and facility sanitation, to minimize the\n potential of microbial contamination in mushrooms.\n","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of microbial safety and cross-contamination sources in\\n button mushroom (Agaricus bisporus)\\n production\",\"authors\":\"Jin-Hee Kim, Song-Yi Choi, Injun Hwang, Jeong-Eun Hyun, S. A. Kim, G. Ban, Se-Ri Kim\",\"doi\":\"10.11002/kjfp.2022.29.7.1079\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n \\n This study aimed to investigate the contamination sources of button mushrooms by\\n assessing the microbial load on the mushrooms and in the agricultural inputs\\n that come into contact with the mushroom. A total of 234 samples including\\n mushrooms, compost, casing soil, and tools from A, B, C, and D farms were tested\\n for sanitary indicators (aerobic bacteria, coliforms, and Escherichia\\n coli) and foodborne pathogens (E. coli O157:H7,\\n Salmonella spp., Staphylococcus aureus,\\n and Listeria monocytogenes). To identify the contamination\\n source, E. coli was isolated and analyzed using MLST\\n (Multi-Locus Sequence Typing). The number of aerobic bacteria remained\\n consistent during composting (⟩7 log CFU/g); however, the occurrence of\\n coliforms and E. coli was reduced to the detection limit\\n (⟨1.0 log). Salmonella spp. and L.\\n monocytogenes were detected in the raw material; however, they were\\n inactivated during composing. More than 6.0 log of aerobic bacteria and 3.0 log\\n of coliforms were observed in the casing soil from A, B, and C farms and in the\\n gloves from the C farm. MLST indicated that the STs of E. coli\\n isolated from casing soil layer and from the harvested mushrooms were identical\\n at ST 189. Therefore, cross contamination occurred through the casing soil\\n layer, and this could contribute to E. coli contamination in\\n mushrooms. It is necessary to practice hygiene guidelines, such as sterilization\\n of casing soil, worker hygiene, and facility sanitation, to minimize the\\n potential of microbial contamination in mushrooms.\\n\",\"PeriodicalId\":17875,\"journal\":{\"name\":\"Korean Journal of Food Preservation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Korean Journal of Food Preservation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11002/kjfp.2022.29.7.1079\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Food Preservation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11002/kjfp.2022.29.7.1079","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Evaluation of microbial safety and cross-contamination sources in
button mushroom (Agaricus bisporus)
production
This study aimed to investigate the contamination sources of button mushrooms by
assessing the microbial load on the mushrooms and in the agricultural inputs
that come into contact with the mushroom. A total of 234 samples including
mushrooms, compost, casing soil, and tools from A, B, C, and D farms were tested
for sanitary indicators (aerobic bacteria, coliforms, and Escherichia
coli) and foodborne pathogens (E. coli O157:H7,
Salmonella spp., Staphylococcus aureus,
and Listeria monocytogenes). To identify the contamination
source, E. coli was isolated and analyzed using MLST
(Multi-Locus Sequence Typing). The number of aerobic bacteria remained
consistent during composting (⟩7 log CFU/g); however, the occurrence of
coliforms and E. coli was reduced to the detection limit
(⟨1.0 log). Salmonella spp. and L.
monocytogenes were detected in the raw material; however, they were
inactivated during composing. More than 6.0 log of aerobic bacteria and 3.0 log
of coliforms were observed in the casing soil from A, B, and C farms and in the
gloves from the C farm. MLST indicated that the STs of E. coli
isolated from casing soil layer and from the harvested mushrooms were identical
at ST 189. Therefore, cross contamination occurred through the casing soil
layer, and this could contribute to E. coli contamination in
mushrooms. It is necessary to practice hygiene guidelines, such as sterilization
of casing soil, worker hygiene, and facility sanitation, to minimize the
potential of microbial contamination in mushrooms.
期刊介绍:
This journal aims to promote and encourage the advancement of quantitative improvement for the storage, processing and distribution of food and its related disciplines, theory and research on its application. Topics covered include: Food Preservation and Packaging Food and Food Material distribution Fresh-cut Food Manufacturing Food processing Technology Food Functional Properties Food Quality / Safety.