用塑化替代方法保存山羊内脏

Q4 Medicine
N. Sultana, M. Khan, T. Amin, I. Uddin, J. Owolabi, V. O., Adeteye, O. Fabiyi, S. Olatunji, J. A. Olanrewaju, A. Obaoye, M. Cook, P. Hill, T. S. I Komarnitki, D. Dziedzic, M. Grzegorczyk, B. Ciszek
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引用次数: 1

摘要

摘要:塑化是生物组织长期保存的过程。这一过程因其作为解剖学教学和研究工具的好处而越来越受欢迎。该工艺是通过强制浸渍代替水和脂肪,再用中间溶剂代替水,生产出坚硬、干燥、无味的样品。然而,由于塑化需要专门的设备和昂贵的化学品,例如聚合物,我们希望简化过程并将样品完全嵌入石蜡中。在这个过程中,水和脂肪被石蜡代替,得到的标本可以触摸,没有气味或腐烂,甚至保留了原始样品的大部分特性。采用新鲜采集的山羊各种内脏器官制备标本。这些器官被解剖出来,暴露出不同的大体解剖特征。标本用10%福尔马林固定,深度冷冻硬化,冷冻干燥机脱水干燥,用融化的石蜡浸渍,固化,室温保存,作为教育工具。所制备的蜡浸渍标本干净、干燥、无味、耐用、无毒,可徒手处理,不需要任何特殊的储存护理。这种方法将加强表面解剖的外观、清晰度和其他部分的描述,以及在本科教学中的实际演示,并增强解剖博物馆的收藏。这些标本的解剖准确性和耐用性使它们成为加速知识获取的有力工具,并加强兽医学生利用更广泛的学习策略的诊断能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preservation of Internal Organs of Goat by An Alternative Method to Plastination
2 Department of Biotechnology, Bangladesh Institute of Nuclear Agency, Mymensingh-2202, Bangladesh ABSTRACT: Plastination is a process of long-term preservation of biological tissue. This process is gaining popularity for its benefits as a teaching and research tool in anatomy. The process is based on replacement of water and fat by forced impregnation, after replacing the water by an intermediate solvent, to produce hard, dry and odorless specimens. However, due to the specialized equipment and expensive chemicals, such as polymer, that are needed for plastination, we desired to simplify the process and embed the samples completely in paraffin wax. In this process, the water and fat were replaced by paraffin, yielding specimens that can be touched, that do not smell or decay, and that even retain most properties of the original sample. Various freshlycollected visceral organs of goat were used for preparing specimens. These organs were dissected out to expose different gross anatomical features. The specimens were fixed in 10% formalin, hardened in a deep freeze, dehydrated and dried in freeze dryer, impregnated with melted paraffin, cured, and then stored at room temperature for further use as educational tool. The prepared wax-impregnated specimens were clean, dry, odorless, durable, non-toxic, and can be handled by bare hands and do not require any special storage care. This method will strengthen the appearance, clarity of surface anatomy, and description of other parts, as well as practical demonstrations in undergraduate teaching, and enhance the anatomical museum collection. The anatomical accuracy and durability of these specimens make them powerful tools to accelerate knowledge acquisition, and strengthens diagnostic abilities for veterinary students utilizing a wider variety of learning strategies.
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来源期刊
Journal of Plastination
Journal of Plastination Health Professions-Medical Laboratory Technology
CiteScore
0.40
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