密苏里州野李褐腐病的发生

IF 1.7 Q2 PLANT SCIENCES

Plant Health Progress Pub Date : 2022-09-04 DOI:10.1094/php-07-22-0061-br

J. Patel, Peng Tian, N. Navarrete-Tindall, W. S. Bartelette

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引用次数: 2

摘要

美国李子(野李子)常见于密苏里州和从德克萨斯州到马萨诸塞州的许多其他美国州。2022年5月，在密苏里州科尔县观察到美洲葡萄果实出现褐腐病症状。症状包括褐腐病和水果干枯。晚期症状包括水果被真菌覆盖。从表现眉腐病症状的果实中分离出一株单孢子菌(PAM-1)。在马铃薯葡萄糖琼脂(PDA)上培养的PAM-1分离物在直径100 mm的培养皿中生长7天。菌落颜色为灰白色至浅棕色，呈圆形，呈同心圆环。菌落边缘完整，在PDA上产生丰富的分生孢子。分生孢子(n=50)长12.07µm，宽7.64µm。分生孢子长圆形或柠檬形，透明。与ITS1和ITS4引物聚合酶链反应生成508 bp的DNA序列，100%鉴定为果实念菌(GenBank登录号:FJ515894.1和EF207419.1)。伤果和未伤果的致病性试验均表现出相似的症状。对照水果没有表现出任何症状。根据病征、病原菌形态、分子特征及致病性鉴定，鉴定为果霉分枝杆菌。据我们所知，这是在密苏里州首次发现果霉菌是引起野李褐腐病的病原菌。

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Occurrence of brown rot of wild plum caused by Monilinia fructicola in Missouri

Prunus americana (wild plum) is commonly found in Missouri and many other US states from Texas to Massachusetts. In May 2022, P. americana fruits exhibiting brown rot symptoms were observed in Cole County, Missouri. The symptoms include brown rot and mummified fruits. Advanced symptoms include fruits covered with fungal growth. A single-spore isolate (PAM-1) was recovered from fruits showing brow rot symptoms. The PAM-1 isolate cultured on potato dextrose agar (PDA) grew a 100 mm diameter Petri dish in seven days. The colony was off-white to light brown in color, circular, and showed concentric rings. The colony had entire margins and produced abundant conidia on PDA. The conidia (n=50) were 12.07 µm long and 7.64 µm wide. The conidia are oblong to lemon-shaped and hyaline. The polymerase chain reaction with ITS1 and ITS4 primers generated 508 bp DNA sequence which showed 100% identity to Monilinia fructicola (GenBank Accession Nos. FJ515894.1 and EF207419.1). Pathogenicity tests on both wounded and unwounded fruits exhibited similar symptoms. Control fruits did not show any symptoms. Based on the disease symptoms, pathogen morphology, molecular characterization, and pathogenicity test the pathogen was identified as M. fructicola. To our knowledge, this is the first scientific report showing M. fructicola as a causal organism of brown rot of wild plum in Missouri.

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来源期刊

Plant Health Progress Agricultural and Biological Sciences-Horticulture

CiteScore

3.00

自引率

13.00%

发文量

期刊介绍： Plant Health Progress, a member journal of the Plant Management Network, is a multidisciplinary science-based journal covering all aspects of applied plant health management in agriculture and horticulture. Both peer-reviewed and fully citable, the journal is a credible online-only publication. Plant Health Progress is a not-for-profit collaborative endeavor of the plant health community at large, serving practitioners worldwide. Its primary goal is to provide a comprehensive one-stop Internet resource for plant health information.