HullRadSAS重述大分子水合作用

IF 2.2 4区生物学 Q3 BIOPHYSICS

European Biophysics Journal Pub Date : 2023-01-05 DOI:10.1007/s00249-022-01627-8

Patrick J. Fleming, John J. Correia, Karen G. Fleming

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引用次数: 11

摘要

生物大分子的水合作用对其稳定性和功能至关重要。历史上，人们曾试图在一个狭窄的数值范围内使用单一参数来描述大分子水合作用的程度。在这里，我们描述了一种方法来计算两种类型的水化:表面壳水和夹带水。考虑这两种类型的水化有助于解释水动力学中的“水化问题”。结合这两种类型的水化作用，可以精确计算水动力体积和相关的大分子特性，如沉降和扩散系数、固有粘度，以及通过沉降速度实验确定的与浓度相关的非理想性。

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Revisiting macromolecular hydration with HullRadSAS

Hydration of biological macromolecules is important for their stability and function. Historically, attempts have been made to describe the degree of macromolecular hydration using a single parameter over a narrow range of values. Here, we describe a method to calculate two types of hydration: surface shell water and entrained water. A consideration of these two types of hydration helps to explain the “hydration problem” in hydrodynamics. The combination of these two types of hydration allows accurate calculation of hydrodynamic volume and related macromolecular properties such as sedimentation and diffusion coefficients, intrinsic viscosities, and the concentration-dependent non-ideality identified with sedimentation velocity experiments.

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来源期刊

European Biophysics Journal 生物-生物物理

CiteScore

4.30

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal publishes papers in the field of biophysics, which is defined as the study of biological phenomena by using physical methods and concepts. Original papers, reviews and Biophysics letters are published. The primary goal of this journal is to advance the understanding of biological structure and function by application of the principles of physical science, and by presenting the work in a biophysical context. Papers employing a distinctively biophysical approach at all levels of biological organisation will be considered, as will both experimental and theoretical studies. The criteria for acceptance are scientific content, originality and relevance to biological systems of current interest and importance. Principal areas of interest include: - Structure and dynamics of biological macromolecules - Membrane biophysics and ion channels - Cell biophysics and organisation - Macromolecular assemblies - Biophysical methods and instrumentation - Advanced microscopics - System dynamics.