荧光成像和生物正交反应在生物分析中的应用

IF 7.1 2区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Topics in Current Chemistry Pub Date : 2021-02-05 DOI:10.1007/s41061-020-00323-5

Yuexiang Ma, Qinhua Chen, Xiaoyan Pan, Jie Zhang

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引用次数: 18

摘要

荧光成像是生物医学领域的一种重要方法。荧光成像具有无损、高效、灵敏、高分辨率、实时动态监测活细胞等特点。然而，它也有一些缺点，如高背景信号和低选择性。生物正交反应具有无损和有效的优点，可用于体内生物相互作用的追踪和分析。本文就荧光探针的作用机制的研究进展作一综述。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Insight into Fluorescence Imaging and Bioorthogonal Reactions in Biological Analysis

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Insight into Fluorescence Imaging and Bioorthogonal Reactions in Biological Analysis

Fluorescence imaging is an important method in the field of biomedicine. Fluorescence imaging is nondestructive, has high efficiency and sensitivity, high resolution and allows real-time dynamic monitoring of living cells. However, it also has some disadvantages, such as high background signals and low selectivity. Bioorthogonal reactions, with the advantages of being both nondestructive and effective, are used to trace and analyze biological interactions in vivo. This review focuses on recent progress in understanding the mechanism of action of fluorescence probes.

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来源期刊

Topics in Current Chemistry Chemistry-General Chemistry

CiteScore

13.70

自引率

1.20%

发文量

期刊介绍： Topics in Current Chemistry is a journal that presents critical reviews of present and future trends in modern chemical research. It covers all areas of chemical science, including interactions with related disciplines like biology, medicine, physics, and materials science. The articles in this journal are organized into thematic collections, offering a comprehensive perspective on emerging research to non-specialist readers in academia or industry. Each review article focuses on one aspect of the topic and provides a critical survey, placing it in the context of the collection. Selected examples highlight significant developments from the past 5 to 10 years. Instead of providing an exhaustive summary or extensive data, the articles concentrate on methodological thinking. This approach allows non-specialist readers to understand the information fully and presents the potential prospects for future developments.