Jin Hong Park, S. Jo, Da Hee Lee, Youngmin Park, H. Chang, T. Kang, K. Lee
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Quantification of triterpenes in Centella asiatica
cultivated in a smart farm, and their effect on keratinocyte
activation
This study aimed to compare the bioactive compounds in Centella
asiatica (C. asiatica) cultivated in a smart farm
and a field and their effects on human keratinocyte cells. C.
asiatica was collected in Jeju-do, Korea, and cultured in a smart
farm and a field. The main bioactive compounds in the two differentially
cultured C. asiatica were identified, and their activation in
keratinocytes were assessed. Amplification and sequencing of the internal
transcribed spacer (ITS) DNA in the nucleus and psbA-H DNA in
the chloroplast were performed for species analysis. A comparison of DNA of
plants reported in the NCBI GenBank was performed. The ITS DNA and
psbA-H DNA sequences of C. asiatica
cultivated in a smart farm and a field were consistent with No.
MH768338.1 and No. JQ425422.1, respectively. Analysis of the triterpenes was
performed using high performance liquid chromatography (HPLC) and as a result,
C. asiatica cultured in a smart farm had more triterpenes
than those cultured in a field. The effects of C. asiatica
grown in a smart farm on cell proliferation and scratch recovery in HaCaT cells
were greater than those grown in a field. These results suggest that C.
asiatica cultivated in a smart farm can be effectively utilized as
a health functional food.
期刊介绍:
This journal aims to promote and encourage the advancement of quantitative improvement for the storage, processing and distribution of food and its related disciplines, theory and research on its application. Topics covered include: Food Preservation and Packaging Food and Food Material distribution Fresh-cut Food Manufacturing Food processing Technology Food Functional Properties Food Quality / Safety.