{"title":"Paraburkholderia sp. pfn29深层发酵生产聚羟基烷烃酸酯(PHAs)及优化","authors":"Thayat Sriyapai , Thitima Chuarung , Kazuhide Kimbara , Siritron Samosorn , Pichapak Sriyapai","doi":"10.1016/j.ejbt.2021.12.003","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Polyhydroxybutyrate (PHB) and polyhydroxybutyrate-<em>co</em>-hydroxyvalerate (PHBV) are the most common polyhydroxyalkanoates (PHAs). They are important candidates for biodegradable plastics that accumulate in the cytoplasm in the form of intracellular granules under unbalanced growth conditions. The present study focused on the production and optimization of PHAs from <em>Paraburkholderia</em> sp. PFN29 for the first time under submerged fermentation.</p></div><div><h3>Results</h3><p>Two hundred bacterial isolates were collected from soil samples in Thailand, six of which were found to accumulate PHB using the Nile red staining method. Six isolates were identified by 16S rDNA gene sequencing and were shown to be closely related to the genera <em>Azotobacter</em>, <em>Sphingobium</em> and <em>Paraburkholderia</em>. The best strain, <em>Paraburkholderia</em> sp. PFN29, showed the highest PHB concentration (5 ± 0.17 g/L). Optimization of PHB accumulation was achieved in modified medium containing 3% (w/v) glucose, and 0.1% (w/v) (NH<sub>4</sub>)<sub>2</sub>Fe(SO<sub>4</sub>) with incubation at pH 7.0 and 35°C for 96 h with shaking at 200 rpm. In preliminary PHBV synthesis, PFN29 was utilized to synthesize PHBV from glucose and organic acids (levulinic acid and propionic acid). <sup>1</sup>H nuclear magnetic resonance (<sup>1</sup>H NMR) spectroscopy and gas chromatography-mass spectrometry (GC/MS) analyses confirmed the extracted PHAs compared to standard PHB and PHBV.</p></div><div><h3>Conclusions</h3><p>PFN29 can be used for feasible PHB production with a PHB content of up to 97.3% of 5.14 ± 0.17 g/L CDW. The PHBV concentration can be further improved by optimizing the production parameters as cosubstrates. The present study provides useful data on PHA production by <em>Paraburkholderia</em>, which may be used as a candidate species for commercial PHA production.</p></div><div><h3>How to cite</h3><p>Sriyapai T, Chuarung T, Kimbara K, et al. Production and optimization of polyhydroxyalkanoates (PHAs) from <em>Paraburkholderia</em> sp. PFN 29 under submerged fermentation. Electron J Biotechnol 2022;56. https://doi.org/10.1016/j.ejbt.2021.12.003</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"56 ","pages":"Pages 1-11"},"PeriodicalIF":2.3000,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345821000592/pdfft?md5=b16df9402cb1d884ce5c9c2b9162184f&pid=1-s2.0-S0717345821000592-main.pdf","citationCount":"12","resultStr":"{\"title\":\"Production and optimization of polyhydroxyalkanoates (PHAs) from Paraburkholderia sp. PFN 29 under submerged fermentation\",\"authors\":\"Thayat Sriyapai , Thitima Chuarung , Kazuhide Kimbara , Siritron Samosorn , Pichapak Sriyapai\",\"doi\":\"10.1016/j.ejbt.2021.12.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Polyhydroxybutyrate (PHB) and polyhydroxybutyrate-<em>co</em>-hydroxyvalerate (PHBV) are the most common polyhydroxyalkanoates (PHAs). They are important candidates for biodegradable plastics that accumulate in the cytoplasm in the form of intracellular granules under unbalanced growth conditions. The present study focused on the production and optimization of PHAs from <em>Paraburkholderia</em> sp. PFN29 for the first time under submerged fermentation.</p></div><div><h3>Results</h3><p>Two hundred bacterial isolates were collected from soil samples in Thailand, six of which were found to accumulate PHB using the Nile red staining method. Six isolates were identified by 16S rDNA gene sequencing and were shown to be closely related to the genera <em>Azotobacter</em>, <em>Sphingobium</em> and <em>Paraburkholderia</em>. The best strain, <em>Paraburkholderia</em> sp. PFN29, showed the highest PHB concentration (5 ± 0.17 g/L). Optimization of PHB accumulation was achieved in modified medium containing 3% (w/v) glucose, and 0.1% (w/v) (NH<sub>4</sub>)<sub>2</sub>Fe(SO<sub>4</sub>) with incubation at pH 7.0 and 35°C for 96 h with shaking at 200 rpm. In preliminary PHBV synthesis, PFN29 was utilized to synthesize PHBV from glucose and organic acids (levulinic acid and propionic acid). <sup>1</sup>H nuclear magnetic resonance (<sup>1</sup>H NMR) spectroscopy and gas chromatography-mass spectrometry (GC/MS) analyses confirmed the extracted PHAs compared to standard PHB and PHBV.</p></div><div><h3>Conclusions</h3><p>PFN29 can be used for feasible PHB production with a PHB content of up to 97.3% of 5.14 ± 0.17 g/L CDW. The PHBV concentration can be further improved by optimizing the production parameters as cosubstrates. The present study provides useful data on PHA production by <em>Paraburkholderia</em>, which may be used as a candidate species for commercial PHA production.</p></div><div><h3>How to cite</h3><p>Sriyapai T, Chuarung T, Kimbara K, et al. Production and optimization of polyhydroxyalkanoates (PHAs) from <em>Paraburkholderia</em> sp. PFN 29 under submerged fermentation. Electron J Biotechnol 2022;56. https://doi.org/10.1016/j.ejbt.2021.12.003</p></div>\",\"PeriodicalId\":11529,\"journal\":{\"name\":\"Electronic Journal of Biotechnology\",\"volume\":\"56 \",\"pages\":\"Pages 1-11\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2022-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0717345821000592/pdfft?md5=b16df9402cb1d884ce5c9c2b9162184f&pid=1-s2.0-S0717345821000592-main.pdf\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Electronic Journal of Biotechnology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0717345821000592\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electronic Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0717345821000592","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Production and optimization of polyhydroxyalkanoates (PHAs) from Paraburkholderia sp. PFN 29 under submerged fermentation
Background
Polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-hydroxyvalerate (PHBV) are the most common polyhydroxyalkanoates (PHAs). They are important candidates for biodegradable plastics that accumulate in the cytoplasm in the form of intracellular granules under unbalanced growth conditions. The present study focused on the production and optimization of PHAs from Paraburkholderia sp. PFN29 for the first time under submerged fermentation.
Results
Two hundred bacterial isolates were collected from soil samples in Thailand, six of which were found to accumulate PHB using the Nile red staining method. Six isolates were identified by 16S rDNA gene sequencing and were shown to be closely related to the genera Azotobacter, Sphingobium and Paraburkholderia. The best strain, Paraburkholderia sp. PFN29, showed the highest PHB concentration (5 ± 0.17 g/L). Optimization of PHB accumulation was achieved in modified medium containing 3% (w/v) glucose, and 0.1% (w/v) (NH4)2Fe(SO4) with incubation at pH 7.0 and 35°C for 96 h with shaking at 200 rpm. In preliminary PHBV synthesis, PFN29 was utilized to synthesize PHBV from glucose and organic acids (levulinic acid and propionic acid). 1H nuclear magnetic resonance (1H NMR) spectroscopy and gas chromatography-mass spectrometry (GC/MS) analyses confirmed the extracted PHAs compared to standard PHB and PHBV.
Conclusions
PFN29 can be used for feasible PHB production with a PHB content of up to 97.3% of 5.14 ± 0.17 g/L CDW. The PHBV concentration can be further improved by optimizing the production parameters as cosubstrates. The present study provides useful data on PHA production by Paraburkholderia, which may be used as a candidate species for commercial PHA production.
How to cite
Sriyapai T, Chuarung T, Kimbara K, et al. Production and optimization of polyhydroxyalkanoates (PHAs) from Paraburkholderia sp. PFN 29 under submerged fermentation. Electron J Biotechnol 2022;56. https://doi.org/10.1016/j.ejbt.2021.12.003
期刊介绍：
Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology.
The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th).
The following areas are covered in the Journal:
• Animal Biotechnology
• Biofilms
• Bioinformatics
• Biomedicine
• Biopolicies of International Cooperation
• Biosafety
• Biotechnology Industry
• Biotechnology of Human Disorders
• Chemical Engineering
• Environmental Biotechnology
• Food Biotechnology
• Marine Biotechnology
• Microbial Biotechnology
• Molecular Biology and Genetics
•Nanobiotechnology
• Omics
• Plant Biotechnology
• Process Biotechnology
• Process Chemistry and Technology
• Tissue Engineering
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