以农业废弃物为底物优化本地分离曲霉菌生产α-淀粉酶

M. Abdulsalam
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引用次数: 0

摘要

这项调查的目的是看看是否有可能从农业废弃物(花生壳)中生产α-淀粉酶。鉴定为曲霉属的菌株(M1)显示出最大的清除区(1.6cm),并用于发酵研究。发酵24小时后,α-淀粉酶活性增加,在第5天达到72.3U/mL的峰值,然后开始下降。利用OFAT研究了最佳环境条件的影响,发现pH 6、接种量为1×107孢子/mL、培养时间为120h、底物浓度为3%(w/v)、温度为35oC是利用曲霉菌从花生壳中生产α-淀粉酶的最佳条件,实验得到最适产酶量为121.3U/mL。这项研究表明,花生壳是一种低成本且普遍可用的废物,可能是制造增值产品的理想基质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimizing α-amylase Production from locally Isolated Aspergillus species Using Selected Agro Waste as Substrate
The goal of this investigation was to see if it was possible to produce α-amylase from agricultural waste (groundnut shell). The strain (M1) identified as Aspergillus sp. exhibited the largest clearance zone (1.6 cm) and was used in fermentation studies. The activity of α-amylase increased after 24 hours of fermentation, peaked at 72.3 U/mL on day 5, and then began to decline. The effect of optimized environmental conditions studied using OFAT, and it was discovered that pH 6, inoculum size of 1 × 107 spores/mL, incubation period of 120 h, substrate concentration of 3 percent (w/v), and temperature of 35oC were the best for producing α-amylase from groundnut shell using Aspergillus sp. In a single fermentation, these optimum conditions were used, and the experiment yielded an optimum enzyme yield of 121.3 U/mL. This research shows that groundnut shell, a low-cost and commonly available waste, could be an ideal substrate for the manufacture of value-added products.
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来源期刊
Bioscience Biotechnology Research Communications
Bioscience Biotechnology Research Communications BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
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