不同级别胶质瘤中癌基因表达的分子分析

Q4 Biochemistry, Genetics and Molecular Biology

Journal of Integrated OMICS Pub Date : 2018-03-17 DOI:10.5584/JIOMICS.V8I1.242

L. Vijayachandran, S. Xavier, K. Sundaram, L. Biswas, D. Panikar, B. Rajamma, S. Nair, K. Menon

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引用次数: 1

摘要

脑肿瘤的侵袭性归因于参与增殖、代谢和治疗耐药性的多种致癌基因的表达，这些致癌基因与肿瘤进展的潜在相关性尚未得到很好的研究。在本研究中，我们旨在研究参与发病机制的肿瘤靶点与神经胶质瘤分级的关系。通过逆转录聚合酶链式反应（RT-PCR）和测序检测表皮生长因子受体（EGFR）突变体来分析胶质瘤（n=40）。EGFR、EGFR变体III（EGFRvIII）、Lck/Yes新型酪氨酸激酶（LYN）、脾脏酪氨酸激酶（SYK）、胰岛素受体底物1（IRS1）、磷脂酰肌醇3-激酶（PI3K）、，使用实时PCR研究含有Src同源2结构域的肌醇5-磷酸酶1（SHIP1）和葡萄糖转运蛋白3（GLUT3），并通过统计学方法与神经胶质瘤分级进行比较。使用免疫组织化学和蛋白质印迹分析蛋白质表达。在53%的胶质瘤中检测到EGFRvIII，在20%的胶质瘤中外显子4缺失（de4-EGFR）。重要的是，候选癌基因的表达水平显著上调（P

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Molecular analysis of oncogene expressions in different grades of gliomas

The aggressiveness of brain tumors is attributed to the expression of multiple oncogenes involved in proliferation, metabolism and therapeutic resistance whose potential correlation with tumor progression has not been well-studied. In this study, we aimed to investigate the relationship of oncotargets involved in pathogenesis with respect to glioma grades. Gliomas ( n =40) were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and sequencing for the detection of epidermal growth factor receptor ( EGFR ) mutants. Expressions levels of EGFR , EGFR variant III ( EGFRvIII ), Lck/Yes novel tyrosine kinase ( LYN ), Spleen tyrosine kinase ( SYK ), insulin receptor substrate 1 ( IRS1 ), phosphatidylinositol 3-kinase ( PI3K ), Src homology 2 domain-containing inositol 5-phosphatase 1 ( SHIP1 ) and glucose transporter 3 ( GLUT3 ) were studied using real-time PCR and compared against glioma grades via statistical methods. Protein expressions were analyzed using immunohistochemistry and western blotting. EGFRvIII was detected in 53% and exon 4 deletion ( de4 EGFR ) in 20% of gliomas. Importantly, the expressions levels of candidate oncogenes were significantly upregulated ( P

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来源期刊

Journal of Integrated OMICS Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： JIOMICS provides a forum for the publication of original research papers, letters to the editor, short communications, and critical reviews in all branches of pure and applied –omics subjects, such as proteomics, metabolomics, metallomics and genomics. Especial interest is given to papers where more than one –omics subject is covered. Papers are evaluated based on scientific novelty and demonstrated scientific applicability. Original research papers on fundamental studies, and novel sensor and instrumentation development, are especially encouraged. Novel or improved findings in areas such as clinical, medicinal, biological, environmental and materials –omics are welcome.