Giulia Godina, B. Vandenbossche, A. Centurión, Verena Dörfler, Mike Barg, R. Ehlers, C. Molina
{"title":"新的基因分型拯救了旧的表型:异habditis bacteriophora野生型材料的有益性状及其与单核苷酸多态性的关联","authors":"Giulia Godina, B. Vandenbossche, A. Centurión, Verena Dörfler, Mike Barg, R. Ehlers, C. Molina","doi":"10.1163/15685411-bja10255","DOIUrl":null,"url":null,"abstract":"\nThe entomopathogenic nematode (EPN), Heterorhabditis bacteriophora, is a biocontrol agent with almost world-wide distribution, thus providing a huge source of biodiversity available for exploitation in breeding programmes to improve beneficial traits. For management of economically important insect pests, the nematode is mass-produced in liquid culture, formulated with inert carriers, and transported under controlled temperature conditions to maintain its quality. The biocontrol potential of nematode materials is defined by their genetic background. Previous reports have assessed the phenotypic plasticity of H. bacteriophora. Major beneficial traits are field efficacy influenced by their virulence, longevity and field persistence and tolerance to abiotic stresses like oxidative stress, desiccation and extreme low and high temperatures. For several of these traits high heritability values (h2) have been reported, providing a platform for trait improvement by selection. Modern breeding uses molecular markers to track beneficial traits. Recent studies reported hundreds of single nucleotide polymorphisms (SNPs) in H. bacteriophora. This investigation revived a large set of cryopreserved wild types, hybrids and phenotyped materials of H. bacteriophora. Additional phenotypic data on the virulence (LC50) of H. bacteriophora strains against mealworm (Tenebrio molitor) were produced ranging from 1.4 ± 0.3 to 8.0 ± 3.6 nematodes per insect. Markers linked to beneficial traits were used for a comprehensive genotype analysis transferring reported SNP sequence information into the multiplex platform SeqSNP. Specific alleles associated to beneficial traits were identified and 65 materials were classified for the presence of relevant SNPs associated to beneficial traits. This information provides a valuable platform for exploitation of the nematode’s biodiversity for future breeding and selection approaches.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":" ","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"New genotyping rescues old phenotypes: beneficial traits in Heterorhabditis bacteriophora wild type material and association to single nucleotide polymorphisms\",\"authors\":\"Giulia Godina, B. Vandenbossche, A. Centurión, Verena Dörfler, Mike Barg, R. Ehlers, C. Molina\",\"doi\":\"10.1163/15685411-bja10255\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\nThe entomopathogenic nematode (EPN), Heterorhabditis bacteriophora, is a biocontrol agent with almost world-wide distribution, thus providing a huge source of biodiversity available for exploitation in breeding programmes to improve beneficial traits. For management of economically important insect pests, the nematode is mass-produced in liquid culture, formulated with inert carriers, and transported under controlled temperature conditions to maintain its quality. The biocontrol potential of nematode materials is defined by their genetic background. Previous reports have assessed the phenotypic plasticity of H. bacteriophora. Major beneficial traits are field efficacy influenced by their virulence, longevity and field persistence and tolerance to abiotic stresses like oxidative stress, desiccation and extreme low and high temperatures. For several of these traits high heritability values (h2) have been reported, providing a platform for trait improvement by selection. Modern breeding uses molecular markers to track beneficial traits. Recent studies reported hundreds of single nucleotide polymorphisms (SNPs) in H. bacteriophora. This investigation revived a large set of cryopreserved wild types, hybrids and phenotyped materials of H. bacteriophora. Additional phenotypic data on the virulence (LC50) of H. bacteriophora strains against mealworm (Tenebrio molitor) were produced ranging from 1.4 ± 0.3 to 8.0 ± 3.6 nematodes per insect. Markers linked to beneficial traits were used for a comprehensive genotype analysis transferring reported SNP sequence information into the multiplex platform SeqSNP. Specific alleles associated to beneficial traits were identified and 65 materials were classified for the presence of relevant SNPs associated to beneficial traits. 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New genotyping rescues old phenotypes: beneficial traits in Heterorhabditis bacteriophora wild type material and association to single nucleotide polymorphisms
The entomopathogenic nematode (EPN), Heterorhabditis bacteriophora, is a biocontrol agent with almost world-wide distribution, thus providing a huge source of biodiversity available for exploitation in breeding programmes to improve beneficial traits. For management of economically important insect pests, the nematode is mass-produced in liquid culture, formulated with inert carriers, and transported under controlled temperature conditions to maintain its quality. The biocontrol potential of nematode materials is defined by their genetic background. Previous reports have assessed the phenotypic plasticity of H. bacteriophora. Major beneficial traits are field efficacy influenced by their virulence, longevity and field persistence and tolerance to abiotic stresses like oxidative stress, desiccation and extreme low and high temperatures. For several of these traits high heritability values (h2) have been reported, providing a platform for trait improvement by selection. Modern breeding uses molecular markers to track beneficial traits. Recent studies reported hundreds of single nucleotide polymorphisms (SNPs) in H. bacteriophora. This investigation revived a large set of cryopreserved wild types, hybrids and phenotyped materials of H. bacteriophora. Additional phenotypic data on the virulence (LC50) of H. bacteriophora strains against mealworm (Tenebrio molitor) were produced ranging from 1.4 ± 0.3 to 8.0 ± 3.6 nematodes per insect. Markers linked to beneficial traits were used for a comprehensive genotype analysis transferring reported SNP sequence information into the multiplex platform SeqSNP. Specific alleles associated to beneficial traits were identified and 65 materials were classified for the presence of relevant SNPs associated to beneficial traits. This information provides a valuable platform for exploitation of the nematode’s biodiversity for future breeding and selection approaches.
期刊介绍:
Nematology is an international journal for the publication of all aspects of nematological research (with the exception of vertebrate parasitology), from molecular biology to field studies. Papers on nematode parasites of arthropods, and on soil free-living nematodes, and on interactions of these and other organisms, are particularly welcome. Research on fresh water and marine nematodes is also considered when the observations are of more general interest.
Nematology publishes full research papers, short communications, Forum articles (which permit an author to express a view on current or fundamental subjects), perspectives on nematology, and reviews of books and other media.