利用硝酸还原酶测定生物源纳米银抑制分枝杆菌

Q4 Chemistry

International Journal of Nano and Biomaterials Pub Date : 2019-08-23 DOI:10.1504/IJNBM.2019.10023327

Adebayo J. Bello, L. Adams, N. Onyejepu, Justice I. Igbinehi, Omoboyede F. Igbari, J. Okpuzor

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引用次数: 0

摘要

以辣木（MO-AgNPs）叶和洋葱（AC-AgNPs）的水提取物为还原剂和封端剂，通过生物还原合成了纳米银。用紫外/可见光谱、红外光谱和SEM-EDX对纳米银进行了表征。采用硝酸还原酶法（NRA）研究了5株分枝杆菌的微生物活性：ATCC 35882、ATCC 25177、非结核分枝杆菌（MOTT）、偶然分枝杆菌和耐多药结核病分枝杆菌（MDR-MTB）。对于UV/vis获得的结果给出了MO-AgNP在430nm处和AC-AgNPs在440nm处的最大吸收。FT-IR显示了与生物分子封端剂相关的官能团。SEM显示纳米银形成的聚集体呈球形和/或椭圆形，表面光滑。EDX光谱显示元素银的存在。两种纳米银对分枝杆菌的最小抑制浓度（MIC）研究表明，与AC AgNPs相比，MO AgNPs具有更高的抑制活性。在此，我们报道了首次使用NRA生物合成的纳米银对分枝杆菌的活性。因此，纳米银可以被开发为纳米气溶胶，用于有针对性和有效治疗分枝杆菌感染。

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Biogenic nanosilver inhibits Mycobacteria using nitrate reductase assay

Nanosilver was synthesised by bioreduction using aqueous extracts of Moringa oleifera (MO-AgNPs) leaf and Allium cepa bulb (AC-AgNPs) as reducing and capping agents. The nanosilver was characterised with UV/vis, FT-IR, and SEM-EDX. Microbiological activity on five Mycobacteria strains: ATCC 35882, ATCC 25177, Mycobacterium other than tuberculosis (MOTT), Mycobacterium fortuitum and multi drug resistance M. tuberculosis (MDR-MTB) was investigated using nitrate reductase assay (NRA). Results obtained for UV/vis gave absorption maxima at 430 nm for MO-AgNPs and 440 nm for AC-AgNPs. FT-IR showed functional groups associated with the biomolecular capping agents. SEM showed the nanosilver formed aggregates spherical and/or in oval shape with smooth surface. EDX spectra exhibited the presence of elemental silver. Minimum inhibitory concentration (MIC) study for both nanosilver against Mycobacteria indicated a higher inhibitory activity for MO-AgNPs over the AC-AgNPs. Herein, we have reported the first biosynthesised nanosilver activity against Mycobacteria using NRA. Nanosilver may therefore be developed as nanoaerosol for targeted and effective treatment of Mycobacteria infections.

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来源期刊

International Journal of Nano and Biomaterials Chemistry-Physical and Theoretical Chemistry

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： In recent years, frontiers of research in engineering, science and technology have been driven by developments in nanomaterials, encompassing a diverse range of disciplines such as materials science, biomedical engineering, nanomedicine and biology, manufacturing technology, biotechnology, nanotechnology, and nanoelectronics. IJNBM provides an interdisciplinary vehicle covering these fields. Advanced materials inspired by biological systems and processes are likely to influence the development of novel technologies for a wide variety of applications from vaccines to artificial tissues and organs to quantum computers. Topics covered include Nanostructured materials/surfaces/interfaces Synthesis of nanostructures Biological/biomedical materials Artificial organs/tissues Tissue engineering Bioengineering materials Medical devices Functional/structural nanomaterials Carbon-based materials Nanomaterials characterisation Novel applications of nanomaterials Modelling of behaviour of nanomaterials Nanomaterials for biomedical applications Biological response to nanomaterials.