{"title":"石斛核因子- y基因家族系统发育分析及表达谱分析。(兰科)","authors":"Devina Ghai , Jaspreet K. Sembi","doi":"10.1016/j.plgene.2022.100365","DOIUrl":null,"url":null,"abstract":"<div><p><span>Nuclear factor-Y (NF-Y) are transcription factors that play vital role in various developmental processes such as flowering<span>, embryogenesis<span>, root formation, nodulation and stress tolerance. In the present study, 27 </span></span></span><em>NF-Y</em> genes were identified in <span><em>Dendrobium</em><em> catenatum</em></span><span><span> which were grouped into three sub-families: DcNF-YA (5), DcNF-YB (10) and DcNF-YC (12), on the basis of presence of specific conserved domains. The phylogenetic relationship between DcNF-Y </span>protein sequences with their orthologs in </span><span><em>Vanilla planifolia</em></span> and <span><em>Arabidopsis thaliana</em></span> confirmed the classification of these proteins into the specified three groups. Physico-chemical characterization reported a wide range in the protein length (111 to 433 amino acids), with DcNF-YAs being longer than the others. Strong interaction between most of the DcNF-Y proteins suggests towards the formation of complexes which might be playing a role in different developmental processes. Gene architectural studies predicted the presence of multiple (3–6) introns in <em>DcNF-YA</em> in contrast with the intron-less status of majority of <em>DcNF-YB</em> and <em>DcNF-YC</em><span> genes. The abundance of stress related cis-regulatory elements in the promoter regions of </span><em>DcNF-Y</em><span> genes indicate the potential of these genes in stress tolerance. Additionally, variable expression of these genes in diverse tissues point towards their widespread role in growth and development. This work can act as a harbinger for functional characterization of </span><em>NF-Y</em> genes with a view for genetic improvement in <em>Dendrobium catenatum</em> Lindl.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phylogenetic analysis and expression profiling of Nuclear Factor-Y gene family in Dendrobium catenatum Lindl. (Orchidaceae)\",\"authors\":\"Devina Ghai , Jaspreet K. Sembi\",\"doi\":\"10.1016/j.plgene.2022.100365\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>Nuclear factor-Y (NF-Y) are transcription factors that play vital role in various developmental processes such as flowering<span>, embryogenesis<span>, root formation, nodulation and stress tolerance. In the present study, 27 </span></span></span><em>NF-Y</em> genes were identified in <span><em>Dendrobium</em><em> catenatum</em></span><span><span> which were grouped into three sub-families: DcNF-YA (5), DcNF-YB (10) and DcNF-YC (12), on the basis of presence of specific conserved domains. The phylogenetic relationship between DcNF-Y </span>protein sequences with their orthologs in </span><span><em>Vanilla planifolia</em></span> and <span><em>Arabidopsis thaliana</em></span> confirmed the classification of these proteins into the specified three groups. Physico-chemical characterization reported a wide range in the protein length (111 to 433 amino acids), with DcNF-YAs being longer than the others. Strong interaction between most of the DcNF-Y proteins suggests towards the formation of complexes which might be playing a role in different developmental processes. Gene architectural studies predicted the presence of multiple (3–6) introns in <em>DcNF-YA</em> in contrast with the intron-less status of majority of <em>DcNF-YB</em> and <em>DcNF-YC</em><span> genes. The abundance of stress related cis-regulatory elements in the promoter regions of </span><em>DcNF-Y</em><span> genes indicate the potential of these genes in stress tolerance. Additionally, variable expression of these genes in diverse tissues point towards their widespread role in growth and development. This work can act as a harbinger for functional characterization of </span><em>NF-Y</em> genes with a view for genetic improvement in <em>Dendrobium catenatum</em> Lindl.</p></div>\",\"PeriodicalId\":38041,\"journal\":{\"name\":\"Plant Gene\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Gene\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2352407322000154\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352407322000154","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
摘要
核因子- y (Nuclear factor-Y, NF-Y)是一种转录因子,在植物开花、胚胎发生、根系形成、结瘤和抗逆性等发育过程中发挥重要作用。本研究共鉴定出27个NF-Y基因,根据其特定保守结构域的存在,将其分为3个亚家族:DcNF-YA(5)、DcNF-YB(10)和DcNF-YC(12)。DcNF-Y蛋白序列与其在planifolia和Arabidopsis thaliana中的同源物之间的系统发育关系证实了这些蛋白可分为三个特定的类群。理化鉴定结果显示,DcNF-YAs的蛋白长度范围很广(111 - 433个氨基酸),其中DcNF-YAs的长度较长。大多数DcNF-Y蛋白之间的强相互作用表明形成复合物可能在不同的发育过程中发挥作用。基因结构研究预测DcNF-YA中存在多个(3-6)内含子,而大多数DcNF-YB和DcNF-YC基因的内含子较少。DcNF-Y基因启动子区域中大量与胁迫相关的顺式调控元件表明这些基因在胁迫耐受方面具有潜力。此外,这些基因在不同组织中的不同表达表明它们在生长发育中的广泛作用。本研究可为石斛NF-Y基因的功能鉴定和遗传改良提供理论依据。
Phylogenetic analysis and expression profiling of Nuclear Factor-Y gene family in Dendrobium catenatum Lindl. (Orchidaceae)
Nuclear factor-Y (NF-Y) are transcription factors that play vital role in various developmental processes such as flowering, embryogenesis, root formation, nodulation and stress tolerance. In the present study, 27 NF-Y genes were identified in Dendrobium catenatum which were grouped into three sub-families: DcNF-YA (5), DcNF-YB (10) and DcNF-YC (12), on the basis of presence of specific conserved domains. The phylogenetic relationship between DcNF-Y protein sequences with their orthologs in Vanilla planifolia and Arabidopsis thaliana confirmed the classification of these proteins into the specified three groups. Physico-chemical characterization reported a wide range in the protein length (111 to 433 amino acids), with DcNF-YAs being longer than the others. Strong interaction between most of the DcNF-Y proteins suggests towards the formation of complexes which might be playing a role in different developmental processes. Gene architectural studies predicted the presence of multiple (3–6) introns in DcNF-YA in contrast with the intron-less status of majority of DcNF-YB and DcNF-YC genes. The abundance of stress related cis-regulatory elements in the promoter regions of DcNF-Y genes indicate the potential of these genes in stress tolerance. Additionally, variable expression of these genes in diverse tissues point towards their widespread role in growth and development. This work can act as a harbinger for functional characterization of NF-Y genes with a view for genetic improvement in Dendrobium catenatum Lindl.
Plant GeneAgricultural and Biological Sciences-Plant Science
CiteScore
4.50
自引率
0.00%
发文量
42
审稿时长
51 days
期刊介绍:
Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.