利用SDS-PAGE分析鸟类品种间蛋蛋白的差异

Q. A. Amin, Hemn Zhahir, A. Shaker
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引用次数: 0

摘要

蛋白质是所有生物体的基本成分;蛋清和蛋黄都是蛋白质的来源。本研究的目的是通过SDS-PAGE(十二烷基硫酸钠-聚丙烯酰胺凝胶电泳)对不同鸟类(珍珠鸡、矮母鸡、地方母鸡、沙米鸡、火鸡、鸭、鹅、鹧鸪和鹌鹑)的蛋清蛋白和蛋黄蛋白进行初步分析和比较。从苏莱曼尼省的各个农场收集了18个不同家禽品种的新鲜鸡蛋(珍珠鸡、矮母鸡、当地母鸡、沙米鸡、火鸡、鸭、鹅、鹧鸪和鹌鹑)。使用Statistical Xlstate进行树突图构建和主成分分析。蛋清蛋白主要有卵黄蛋白、卵黄转铁蛋白、卵黄蛋白、α-凝乳胰蛋白酶原和胰蛋白酶抑制剂。主要脂蛋白有卵黄素VI、卵黄素Vb、卵黄素V、卵黄素IIIa、卵黄素III、卵黄素7、B-Livetin、卵黄素IIa、卵黄素II和卵黄素i。这些脂蛋白在9种鸟类中均有发现。测定了9种昆虫的蛋清蛋白和蛋黄脂蛋白。由此可见,9种鸟类的蛋清蛋白和蛋黄脂蛋白含量存在较大差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Variation of egg proteins between bird varieties by using SDS-PAGE
Proteins are essential constituents of all organisms; both egg white proteins and egg yolk are source of protein. The aim of this study was conducted to perform preliminary studies to analyses and compare egg white proteins and yolk proteins from different avian species (guineafowl, dwarf hens, local hen, Shami, turkey, duck, geese, partridge and quail) via or with SDS-PAGE (Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis ). 18 Fresh eggs of different poultry species (guineafowl, dwarf hens, local hen, Shami, turkey, duck, geese, partridge and quail) were collected from various farms in the Sulaimani province. Data on egg proteins were analyzed using Statistical Xlstate used for dendrogram construction and PCA. The main egg white proteins were Ovomicin, Ovotransferrin, Ovalbumin, Flavoprotein, α- chymotrypsinogen, and Trypsin inhibitor. The main lipoproteins were Apovitellenin VI, Apovitellenin Vb, Apovitellenin V, Apovitellenin IIIa, Apovitellenin III, Apovitellin 7, B-Livetin, Apovitellenin IIa, Apovitellenin II, and Apovitellenin I. All these lipoproteins were observed in the nine birds species. The egg white proteins and yolk lipoproteins for nine species were examined. It can be concluded the large differences were found in a mount of egg white proteins and yolk lipoproteins of the nine species of birds.
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