聚羟基烷酸酯和抗菌/抗病毒金纳米粒子的双重生产

IF 4.1 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY
Alexandra Paxinou, Elena Marcello, Vittoria Vecchiato, Lara Erman, Edward Wright, Brendon Noble, Adele McCormick, Pooja Basnett
{"title":"聚羟基烷酸酯和抗菌/抗病毒金纳米粒子的双重生产","authors":"Alexandra Paxinou, Elena Marcello, Vittoria Vecchiato, Lara Erman, Edward Wright, Brendon Noble, Adele McCormick, Pooja Basnett","doi":"10.3389/fnano.2023.1243056","DOIUrl":null,"url":null,"abstract":"Gold nanoparticles (AuNPs) have been explored for their use in medicine. Here, we report a sustainable, and cost-effective method to produce AuNPs using a bacterial strain such as Pseudomonas mendocina CH50 which is also known to be a polyhydroxyalkanoate (PHA) producer. A cell-free bacterial supernatant, which is typically discarded after PHA extraction, was used to produce spherical AuNPs of 3.5 ± 1.5 nm in size as determined by Transmission Electron Microscopy (TEM) analysis. The AuNPs/PHA composite coating demonstrated antibacterial activity against Staphylococcus aureus 6538P, and antiviral activity, with a 75% reduction in viral infectivity against SARS-CoV-2 pseudotype virus.","PeriodicalId":34432,"journal":{"name":"Frontiers in Nanotechnology","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dual production of polyhydroxyalkanoates and antibacterial/antiviral gold nanoparticles\",\"authors\":\"Alexandra Paxinou, Elena Marcello, Vittoria Vecchiato, Lara Erman, Edward Wright, Brendon Noble, Adele McCormick, Pooja Basnett\",\"doi\":\"10.3389/fnano.2023.1243056\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Gold nanoparticles (AuNPs) have been explored for their use in medicine. Here, we report a sustainable, and cost-effective method to produce AuNPs using a bacterial strain such as Pseudomonas mendocina CH50 which is also known to be a polyhydroxyalkanoate (PHA) producer. A cell-free bacterial supernatant, which is typically discarded after PHA extraction, was used to produce spherical AuNPs of 3.5 ± 1.5 nm in size as determined by Transmission Electron Microscopy (TEM) analysis. The AuNPs/PHA composite coating demonstrated antibacterial activity against Staphylococcus aureus 6538P, and antiviral activity, with a 75% reduction in viral infectivity against SARS-CoV-2 pseudotype virus.\",\"PeriodicalId\":34432,\"journal\":{\"name\":\"Frontiers in Nanotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2023-09-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Nanotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3389/fnano.2023.1243056\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Nanotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3389/fnano.2023.1243056","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

金纳米粒子(AuNPs)已被探索用于医学。在这里,我们报道了一种可持续的、成本效益高的方法,使用细菌菌株如门多西假单胞菌CH50生产AuNPs,门多西菌CH50也是聚羟基烷酸酯(PHA)的生产商。通常在PHA提取后丢弃的无细胞细菌上清液用于产生通过透射电子显微镜(TEM)分析确定的大小为3.5±1.5nm的球形AuNP。AuNPs/PHA复合涂层显示出对金黄色葡萄球菌6538P的抗菌活性和抗病毒活性,对严重急性呼吸系统综合征冠状病毒2型假型病毒的病毒传染性降低了75%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dual production of polyhydroxyalkanoates and antibacterial/antiviral gold nanoparticles
Gold nanoparticles (AuNPs) have been explored for their use in medicine. Here, we report a sustainable, and cost-effective method to produce AuNPs using a bacterial strain such as Pseudomonas mendocina CH50 which is also known to be a polyhydroxyalkanoate (PHA) producer. A cell-free bacterial supernatant, which is typically discarded after PHA extraction, was used to produce spherical AuNPs of 3.5 ± 1.5 nm in size as determined by Transmission Electron Microscopy (TEM) analysis. The AuNPs/PHA composite coating demonstrated antibacterial activity against Staphylococcus aureus 6538P, and antiviral activity, with a 75% reduction in viral infectivity against SARS-CoV-2 pseudotype virus.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Frontiers in Nanotechnology
Frontiers in Nanotechnology Engineering-Electrical and Electronic Engineering
CiteScore
7.10
自引率
0.00%
发文量
96
审稿时长
13 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信