聚羟基烷酸酯和抗菌/抗病毒金纳米粒子的双重生产

IF 4.1 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Frontiers in Nanotechnology Pub Date : 2023-09-07 DOI:10.3389/fnano.2023.1243056

Alexandra Paxinou, Elena Marcello, Vittoria Vecchiato, Lara Erman, Edward Wright, Brendon Noble, Adele McCormick, Pooja Basnett

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引用次数: 0

摘要

金纳米粒子（AuNPs）已被探索用于医学。在这里，我们报道了一种可持续的、成本效益高的方法，使用细菌菌株如门多西假单胞菌CH50生产AuNPs，门多西菌CH50也是聚羟基烷酸酯（PHA）的生产商。通常在PHA提取后丢弃的无细胞细菌上清液用于产生通过透射电子显微镜（TEM）分析确定的大小为3.5±1.5nm的球形AuNP。AuNPs/PHA复合涂层显示出对金黄色葡萄球菌6538P的抗菌活性和抗病毒活性，对严重急性呼吸系统综合征冠状病毒2型假型病毒的病毒传染性降低了75%。

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Dual production of polyhydroxyalkanoates and antibacterial/antiviral gold nanoparticles

Gold nanoparticles (AuNPs) have been explored for their use in medicine. Here, we report a sustainable, and cost-effective method to produce AuNPs using a bacterial strain such as Pseudomonas mendocina CH50 which is also known to be a polyhydroxyalkanoate (PHA) producer. A cell-free bacterial supernatant, which is typically discarded after PHA extraction, was used to produce spherical AuNPs of 3.5 ± 1.5 nm in size as determined by Transmission Electron Microscopy (TEM) analysis. The AuNPs/PHA composite coating demonstrated antibacterial activity against Staphylococcus aureus 6538P, and antiviral activity, with a 75% reduction in viral infectivity against SARS-CoV-2 pseudotype virus.

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来源期刊

Frontiers in Nanotechnology Engineering-Electrical and Electronic Engineering

CiteScore

7.10

自引率

0.00%

发文量

审稿时长

13 weeks