{"title":"两种不动杆菌吲哚降解代谢物的代谢分析。","authors":"T. A. Tesso, G. Liu","doi":"10.1134/S0003683823040154","DOIUrl":null,"url":null,"abstract":"<p>Indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human, animals and ecological environment. Bacteria play vital role in the degradation and biotransformation of such persistent organic and harmful pollutants. In this experiment, <i>Acinetobacter towneri</i> NTA1-2A and <i>Acinetobacter guillouiae</i> TAT1-6A were used to degrade indole. Indole-degradated metabolites were analyzed by metabolomics analysis of fermentation broth of these strains using LC-MS/MS. The metabolomics analysis showed indole-5-carbonitrile 3-benzoyl (C<sub>16</sub>H<sub>10</sub>N<sub>2</sub>O), dimethyl sulfoxide (C<sub>2</sub>H<sub>6</sub>OS), deoxyguanosine (C<sub>10</sub>H<sub>13</sub>N<sub>5</sub>O<sub>4</sub>), Leu (C<sub>6</sub>H<sub>13</sub>NO<sub>2</sub>), and N-nitroso-pyrrolidine were indole-degradated metabolites formed by <i>A. towneri</i> NTA1-2A while neurine (C<sub>5</sub>H<sub>13</sub>NO) and norvaline (C<sub>5</sub>H<sub>11</sub>NO<sub>2</sub>) are common metabolites of both strains. The two strains' degradation metabolites differed from those reported in other studies, demonstrating the diversity of the distinct microbial strains approach to the metabolism of indolic compounds. Extracellular enzymes released to culture medium by these bacteria were also identified. In conclusion, the two bacterial strains have a potential of converting notorious indole compound to non-toxic intermediates mediated by enzymes. Hence, future study should focus on biochemical pathway and genetic basis for indole-degradated metabolites in the two strains.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 4","pages":"462 - 467"},"PeriodicalIF":1.0000,"publicationDate":"2023-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Metabolic Analysis of Indole-degradated Metabolites in Two Acinetobacter spp.\",\"authors\":\"T. A. Tesso, G. Liu\",\"doi\":\"10.1134/S0003683823040154\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human, animals and ecological environment. Bacteria play vital role in the degradation and biotransformation of such persistent organic and harmful pollutants. In this experiment, <i>Acinetobacter towneri</i> NTA1-2A and <i>Acinetobacter guillouiae</i> TAT1-6A were used to degrade indole. Indole-degradated metabolites were analyzed by metabolomics analysis of fermentation broth of these strains using LC-MS/MS. The metabolomics analysis showed indole-5-carbonitrile 3-benzoyl (C<sub>16</sub>H<sub>10</sub>N<sub>2</sub>O), dimethyl sulfoxide (C<sub>2</sub>H<sub>6</sub>OS), deoxyguanosine (C<sub>10</sub>H<sub>13</sub>N<sub>5</sub>O<sub>4</sub>), Leu (C<sub>6</sub>H<sub>13</sub>NO<sub>2</sub>), and N-nitroso-pyrrolidine were indole-degradated metabolites formed by <i>A. towneri</i> NTA1-2A while neurine (C<sub>5</sub>H<sub>13</sub>NO) and norvaline (C<sub>5</sub>H<sub>11</sub>NO<sub>2</sub>) are common metabolites of both strains. The two strains' degradation metabolites differed from those reported in other studies, demonstrating the diversity of the distinct microbial strains approach to the metabolism of indolic compounds. Extracellular enzymes released to culture medium by these bacteria were also identified. In conclusion, the two bacterial strains have a potential of converting notorious indole compound to non-toxic intermediates mediated by enzymes. Hence, future study should focus on biochemical pathway and genetic basis for indole-degradated metabolites in the two strains.</p>\",\"PeriodicalId\":466,\"journal\":{\"name\":\"Applied Biochemistry and Microbiology\",\"volume\":\"59 4\",\"pages\":\"462 - 467\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2023-08-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Biochemistry and Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0003683823040154\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683823040154","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Metabolic Analysis of Indole-degradated Metabolites in Two Acinetobacter spp.
Indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human, animals and ecological environment. Bacteria play vital role in the degradation and biotransformation of such persistent organic and harmful pollutants. In this experiment, Acinetobacter towneri NTA1-2A and Acinetobacter guillouiae TAT1-6A were used to degrade indole. Indole-degradated metabolites were analyzed by metabolomics analysis of fermentation broth of these strains using LC-MS/MS. The metabolomics analysis showed indole-5-carbonitrile 3-benzoyl (C16H10N2O), dimethyl sulfoxide (C2H6OS), deoxyguanosine (C10H13N5O4), Leu (C6H13NO2), and N-nitroso-pyrrolidine were indole-degradated metabolites formed by A. towneri NTA1-2A while neurine (C5H13NO) and norvaline (C5H11NO2) are common metabolites of both strains. The two strains' degradation metabolites differed from those reported in other studies, demonstrating the diversity of the distinct microbial strains approach to the metabolism of indolic compounds. Extracellular enzymes released to culture medium by these bacteria were also identified. In conclusion, the two bacterial strains have a potential of converting notorious indole compound to non-toxic intermediates mediated by enzymes. Hence, future study should focus on biochemical pathway and genetic basis for indole-degradated metabolites in the two strains.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
