巴德勒苹果(Malus domestica L)儿茶酚氧化酶的纯化与鉴定:体外和硅细胞研究

IF 1.6 4区农林科学

International Journal of Food Engineering Pub Date : 2022-07-01 DOI:10.1515/ijfe-2022-0022

Ayhan Çiğdem, U. Güller

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引用次数: 1

摘要

摘要本研究采用丙酮沉淀和亲和层析两步法，从巴德尔苹果(Malus domestica L.)中分离纯化了果实酶促褐变的多酚氧化酶(PPO)。纯化后用SDS-PAGE检测PBPPO的纯度。结果表明，PBPPO在pH 6.0和10°C时活性最高，在pH 5.5和温度0-30°C时稳定。另外，Al3+和Cu2+金属离子对PBPPO有激活作用，抗坏血酸对PBPPO有较强的抑制作用，Ki常数为1.67±0.35µM。通过分子对接研究考察了抑制剂与酶的相互作用，发现抗坏血酸的对接分数最低，为−6.54 kcal/mol。我们想让大家注意到PB苹果，它内外都是红色的，营养成分非常丰富。

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Purification and characterization of catechol oxidase from Posof Badele apple (Malus domestica L): in vitro and in silico studies

Abstract In the current study, polyphenol oxidase (PPO), responsible for enzymatic browning in fruits, was purified from Posof Badele apple (PB) (Malus domestica L.) in two steps as acetone precipitation and affinity chromatography. After purification, the purity of PBPPO was checked by using SDS-PAGE. It was figured out that PBPPO had maximum activity at pH 6.0 and 10 °C and it was stable at pH 5.5 and temperatures of 0–30 °C. Besides, it was determined that Al3+ and Cu2+ metal ions activated the enzyme and the PBPPO was strongly inhibited by ascorbic acid with a Ki constant of 1.67 ± 0.35 µM. Inhibitor-enzyme interactions were examined by molecular docking studies and it was revealed that ascorbic acid had the lowest docking score of −6.54 kcal/mol. We wanted to draw attention to the PB apple, which is red inside as well as outside and very rich in terms of nutrient content.

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来源期刊

International Journal of Food Engineering 农林科学-食品科技

CiteScore

3.20

自引率

0.00%

发文量

审稿时长

3.8 months

期刊介绍： International Journal of Food Engineering is devoted to engineering disciplines related to processing foods. The areas of interest include heat, mass transfer and fluid flow in food processing; food microstructure development and characterization; application of artificial intelligence in food engineering research and in industry; food biotechnology; and mathematical modeling and software development for food processing purposes. Authors and editors come from top engineering programs around the world: the U.S., Canada, the U.K., and Western Europe, but also South America, Asia, Africa, and the Middle East.