Ольга Недзвецька, Уляна Пастух, Ольга Кузьмина де Гутврра, Ірина Пастух, Ірина Соболева, Наталія Сотник
{"title":"一个疗程的褪黑激素注射对视神经形态学和功能改变的影响","authors":"Ольга Недзвецька, Уляна Пастух, Ольга Кузьмина де Гутврра, Ірина Пастух, Ірина Соболева, Наталія Сотник","doi":"10.31288/oftalmolzh202344854","DOIUrl":null,"url":null,"abstract":"Background: Optic atrophy (OA) may be expected in hypopinealism, which is accompanied by degenerative changes in the retina.\n\nPurpose: To assess the impact of a course of injections with melatonin on the morphological and functional optic nerve (ON) changes in rabbits exposed to prolonged around-the-clock light (ATCL) leading to hypopinealism.\n\nMaterial and Methods: Eighty-four rabbits were used in this experimental study. Group 1 (an ATCL group) was composed of 32 animals exposed to ATCL to develop functional hypopinealism. Group 2 (an ATCL+M group) was composed of 29 animals exposed to ATCL but treated with intramuscular melatonin for 14 days. Group 3 (a control group or CG) was composed of 23 intact animals maintained under natural day/night cycle conditions. Groups were subdivided into subgroups based on experimental constructs (1-2 months, 3-5 months, 8-12 months, 18-19 months, 26-28 months). Blood melatonin levels were assessed by commercially available enzyme-linked immunosorbent assay kits. ON specimens were obtained and comprehensively assessed morphologically and morphometrically.\n\nResults: Night-time blood melatonin level in experimental groups was almost six-fold lower than that in controls. Signs of abnormal ON circulation were observed at ≤12 months of ATCL exposure. ON demyelination was observed from months 3-5 of the experiment. Sclerotic and atrophic processes in the ON were observed at 28 months of ATCL exposure. In ATCL26-28 and ATCL+M26-28 subgroups, the mean relative vascular area in the intraorbital ON was significantly reduced compared to CG26-28 (2.01 ± 0.15% and 1.93 ± 0.15%, respectively, versus 3.20 ± 0.13%, р less 0.05). In addition, the mean relative area of the perivascular connective tissue (4.80 ± 0.15% and 4.61 ± 0.17%, respectively) was significantly increased compared to CG26-28 (3.40 ± 0.14%, р less 0.05). Moreover, the mean diameter of the nerve fiber bundle (2.51 ± 0.09 ×10-6 m and 2.73±0.10×10-6 m, respectively) was significantly reduced compared to CG26-28 (3.85±0.14×10-6 m; р less 0.05).\n\nConclusion: The morphological findings (like demyelination of nerve fibers and thinning of nerve fiber bundles of the ON), combined with low blood flow in ON vessels, vascular wall thickening and connective tissue growth, indicated the development of sclerotic atrophy of the ON, in the presence of marked melatonin deficiency, in rabbits exposed to ATCL. The 14-day course melatonin treatment of ATCI-exposed rabbits exerted anti-edematous effects at early time points (less 5 months), until obviously irreversible changes in the ON occurred. However, the course melatonin treatment exerted no impact on the development of OA in animals with persistent, marked hypopinealism developed in the presence of prolonged (28-month) exposure to ATCI.","PeriodicalId":19419,"journal":{"name":"Oftalmologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impact of a course of injections with melatonin on morphological and functional changes in the optic nerve in experimental animals with hypopinealism\",\"authors\":\"Ольга Недзвецька, Уляна Пастух, Ольга Кузьмина де Гутврра, Ірина Пастух, Ірина Соболева, Наталія Сотник\",\"doi\":\"10.31288/oftalmolzh202344854\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Optic atrophy (OA) may be expected in hypopinealism, which is accompanied by degenerative changes in the retina.\\n\\nPurpose: To assess the impact of a course of injections with melatonin on the morphological and functional optic nerve (ON) changes in rabbits exposed to prolonged around-the-clock light (ATCL) leading to hypopinealism.\\n\\nMaterial and Methods: Eighty-four rabbits were used in this experimental study. Group 1 (an ATCL group) was composed of 32 animals exposed to ATCL to develop functional hypopinealism. Group 2 (an ATCL+M group) was composed of 29 animals exposed to ATCL but treated with intramuscular melatonin for 14 days. Group 3 (a control group or CG) was composed of 23 intact animals maintained under natural day/night cycle conditions. Groups were subdivided into subgroups based on experimental constructs (1-2 months, 3-5 months, 8-12 months, 18-19 months, 26-28 months). Blood melatonin levels were assessed by commercially available enzyme-linked immunosorbent assay kits. ON specimens were obtained and comprehensively assessed morphologically and morphometrically.\\n\\nResults: Night-time blood melatonin level in experimental groups was almost six-fold lower than that in controls. Signs of abnormal ON circulation were observed at ≤12 months of ATCL exposure. ON demyelination was observed from months 3-5 of the experiment. Sclerotic and atrophic processes in the ON were observed at 28 months of ATCL exposure. In ATCL26-28 and ATCL+M26-28 subgroups, the mean relative vascular area in the intraorbital ON was significantly reduced compared to CG26-28 (2.01 ± 0.15% and 1.93 ± 0.15%, respectively, versus 3.20 ± 0.13%, р less 0.05). In addition, the mean relative area of the perivascular connective tissue (4.80 ± 0.15% and 4.61 ± 0.17%, respectively) was significantly increased compared to CG26-28 (3.40 ± 0.14%, р less 0.05). Moreover, the mean diameter of the nerve fiber bundle (2.51 ± 0.09 ×10-6 m and 2.73±0.10×10-6 m, respectively) was significantly reduced compared to CG26-28 (3.85±0.14×10-6 m; р less 0.05).\\n\\nConclusion: The morphological findings (like demyelination of nerve fibers and thinning of nerve fiber bundles of the ON), combined with low blood flow in ON vessels, vascular wall thickening and connective tissue growth, indicated the development of sclerotic atrophy of the ON, in the presence of marked melatonin deficiency, in rabbits exposed to ATCL. The 14-day course melatonin treatment of ATCI-exposed rabbits exerted anti-edematous effects at early time points (less 5 months), until obviously irreversible changes in the ON occurred. However, the course melatonin treatment exerted no impact on the development of OA in animals with persistent, marked hypopinealism developed in the presence of prolonged (28-month) exposure to ATCI.\",\"PeriodicalId\":19419,\"journal\":{\"name\":\"Oftalmologicheskii zhurnal\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Oftalmologicheskii zhurnal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31288/oftalmolzh202344854\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oftalmologicheskii zhurnal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31288/oftalmolzh202344854","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
Impact of a course of injections with melatonin on morphological and functional changes in the optic nerve in experimental animals with hypopinealism
Background: Optic atrophy (OA) may be expected in hypopinealism, which is accompanied by degenerative changes in the retina.
Purpose: To assess the impact of a course of injections with melatonin on the morphological and functional optic nerve (ON) changes in rabbits exposed to prolonged around-the-clock light (ATCL) leading to hypopinealism.
Material and Methods: Eighty-four rabbits were used in this experimental study. Group 1 (an ATCL group) was composed of 32 animals exposed to ATCL to develop functional hypopinealism. Group 2 (an ATCL+M group) was composed of 29 animals exposed to ATCL but treated with intramuscular melatonin for 14 days. Group 3 (a control group or CG) was composed of 23 intact animals maintained under natural day/night cycle conditions. Groups were subdivided into subgroups based on experimental constructs (1-2 months, 3-5 months, 8-12 months, 18-19 months, 26-28 months). Blood melatonin levels were assessed by commercially available enzyme-linked immunosorbent assay kits. ON specimens were obtained and comprehensively assessed morphologically and morphometrically.
Results: Night-time blood melatonin level in experimental groups was almost six-fold lower than that in controls. Signs of abnormal ON circulation were observed at ≤12 months of ATCL exposure. ON demyelination was observed from months 3-5 of the experiment. Sclerotic and atrophic processes in the ON were observed at 28 months of ATCL exposure. In ATCL26-28 and ATCL+M26-28 subgroups, the mean relative vascular area in the intraorbital ON was significantly reduced compared to CG26-28 (2.01 ± 0.15% and 1.93 ± 0.15%, respectively, versus 3.20 ± 0.13%, р less 0.05). In addition, the mean relative area of the perivascular connective tissue (4.80 ± 0.15% and 4.61 ± 0.17%, respectively) was significantly increased compared to CG26-28 (3.40 ± 0.14%, р less 0.05). Moreover, the mean diameter of the nerve fiber bundle (2.51 ± 0.09 ×10-6 m and 2.73±0.10×10-6 m, respectively) was significantly reduced compared to CG26-28 (3.85±0.14×10-6 m; р less 0.05).
Conclusion: The morphological findings (like demyelination of nerve fibers and thinning of nerve fiber bundles of the ON), combined with low blood flow in ON vessels, vascular wall thickening and connective tissue growth, indicated the development of sclerotic atrophy of the ON, in the presence of marked melatonin deficiency, in rabbits exposed to ATCL. The 14-day course melatonin treatment of ATCI-exposed rabbits exerted anti-edematous effects at early time points (less 5 months), until obviously irreversible changes in the ON occurred. However, the course melatonin treatment exerted no impact on the development of OA in animals with persistent, marked hypopinealism developed in the presence of prolonged (28-month) exposure to ATCI.
期刊介绍:
Journal of Ophthalmology (Ukraine) contains articles on eye diseases diagnosis and treatment, eye care, eye diseases prevention, history of ophthalmology, organization of eye care to population, technical equipment problems. It is committed to publishing original scientific researches and review articles on theory and practice of Ukrainian and foreign ophthalmology, cases, reviews, inventions, historical and medical reviews. It contains peer-reviews of books on ophthalmology, articles on activity of ophthalmologic societies, congresses and conferences chronicles. Journal is designed to ophthalmologists and scientific researchers in the field of vision physiology and eye diseases clinic.