一个疗程的褪黑激素注射对视神经形态学和功能改变的影响

Q4 Medicine
Ольга Недзвецька, Уляна Пастух, Ольга Кузьмина де Гутврра, Ірина Пастух, Ірина Соболева, Наталія Сотник
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引用次数: 0

摘要

背景:视神经萎缩(OA)可能出现在视功能减退症,并伴有视网膜的退行性改变。目的:评估一个疗程的褪黑激素注射对暴露于长时间光照(ATCL)导致垂体功能低下的家兔视神经(on)形态学和功能变化的影响。材料与方法:84只家兔进行实验研究。第1组(ATCL组)由32只暴露于ATCL导致功能性性功能减退的动物组成。第2组(ATCL+M组)由29只暴露于ATCL但肌肉注射褪黑素14天的动物组成。第3组(对照组或CG)由23只在自然昼夜循环条件下维持的完整动物组成。各组根据实验结构再分为亚组(1-2个月、3-5个月、8-12个月、18-19个月、26-28个月)。血液褪黑激素水平通过市售的酶联免疫吸附测定试剂盒进行评估。获得ON标本并进行形态学和形态计量学综合评估。结果:实验组夜间血液褪黑素水平比对照组低近6倍。ATCL暴露≤12个月时观察到ON循环异常迹象。实验3-5个月观察到ON脱髓鞘。在ATCL暴露28个月时观察到ON的硬化和萎缩过程。ATCL26-28和ATCL+M26-28亚组眶内ON平均相对血管面积较CG26-28显著减少(分别为2.01±0.15%和1.93±0.15%,分别为3.20±0.13%,差异均为0.05)。血管周围结缔组织的平均相对面积分别为4.80±0.15%和4.61±0.17%,较CG26-28(3.40±0.14%,差异均为0.05)显著增加。此外,神经纤维束的平均直径(2.51±0.09 ×10-6 m和2.73±0.10×10-6 m)比CG26-28(3.85±0.14×10-6 m;< 0.05)。结论:ATCL暴露家兔的神经纤维脱髓鞘、神经纤维束变薄、血管血流量低、血管壁增厚、结缔组织生长等形态学表现表明,在褪黑素明显缺乏的情况下,ON发生了硬化性萎缩。对atci暴露家兔进行为期14天的褪黑素治疗,在早期时间点(少于5个月)发挥了抗水肿作用,直到ON发生明显不可逆的变化。然而,在长期(28个月)暴露于ATCI的动物中,褪黑素治疗过程对持续、明显的垂体功能减退症的发展没有影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of a course of injections with melatonin on morphological and functional changes in the optic nerve in experimental animals with hypopinealism
Background: Optic atrophy (OA) may be expected in hypopinealism, which is accompanied by degenerative changes in the retina. Purpose: To assess the impact of a course of injections with melatonin on the morphological and functional optic nerve (ON) changes in rabbits exposed to prolonged around-the-clock light (ATCL) leading to hypopinealism. Material and Methods: Eighty-four rabbits were used in this experimental study. Group 1 (an ATCL group) was composed of 32 animals exposed to ATCL to develop functional hypopinealism. Group 2 (an ATCL+M group) was composed of 29 animals exposed to ATCL but treated with intramuscular melatonin for 14 days. Group 3 (a control group or CG) was composed of 23 intact animals maintained under natural day/night cycle conditions. Groups were subdivided into subgroups based on experimental constructs (1-2 months, 3-5 months, 8-12 months, 18-19 months, 26-28 months). Blood melatonin levels were assessed by commercially available enzyme-linked immunosorbent assay kits. ON specimens were obtained and comprehensively assessed morphologically and morphometrically. Results: Night-time blood melatonin level in experimental groups was almost six-fold lower than that in controls. Signs of abnormal ON circulation were observed at ≤12 months of ATCL exposure. ON demyelination was observed from months 3-5 of the experiment. Sclerotic and atrophic processes in the ON were observed at 28 months of ATCL exposure. In ATCL26-28 and ATCL+M26-28 subgroups, the mean relative vascular area in the intraorbital ON was significantly reduced compared to CG26-28 (2.01 ± 0.15% and 1.93 ± 0.15%, respectively, versus 3.20 ± 0.13%, р less 0.05). In addition, the mean relative area of the perivascular connective tissue (4.80 ± 0.15% and 4.61 ± 0.17%, respectively) was significantly increased compared to CG26-28 (3.40 ± 0.14%, р less 0.05). Moreover, the mean diameter of the nerve fiber bundle (2.51 ± 0.09 ×10-6 m and 2.73±0.10×10-6 m, respectively) was significantly reduced compared to CG26-28 (3.85±0.14×10-6 m; р less 0.05). Conclusion: The morphological findings (like demyelination of nerve fibers and thinning of nerve fiber bundles of the ON), combined with low blood flow in ON vessels, vascular wall thickening and connective tissue growth, indicated the development of sclerotic atrophy of the ON, in the presence of marked melatonin deficiency, in rabbits exposed to ATCL. The 14-day course melatonin treatment of ATCI-exposed rabbits exerted anti-edematous effects at early time points (less 5 months), until obviously irreversible changes in the ON occurred. However, the course melatonin treatment exerted no impact on the development of OA in animals with persistent, marked hypopinealism developed in the presence of prolonged (28-month) exposure to ATCI.
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来源期刊
Oftalmologicheskii zhurnal
Oftalmologicheskii zhurnal Medicine-Ophthalmology
CiteScore
0.40
自引率
0.00%
发文量
71
期刊介绍: Journal of Ophthalmology (Ukraine) contains articles on eye diseases diagnosis and treatment, eye care, eye diseases prevention, history of ophthalmology, organization of eye care to population, technical equipment problems. It is committed to publishing original scientific researches and review articles on theory and practice of Ukrainian and foreign ophthalmology, cases, reviews, inventions, historical and medical reviews. It contains peer-reviews of books on ophthalmology, articles on activity of ophthalmologic societies, congresses and conferences chronicles. Journal is designed to ophthalmologists and scientific researchers in the field of vision physiology and eye diseases clinic.
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