Norimitsu Izawa, Susumu Hiragaki, Amr A. Mohamed, Azza M. Elgendy, Takeshi Ohtani, Makio Takeda
{"title":"芳烷基胺N-乙酰转移酶活性与工作类型及其时间变化平行,表明其参与了蜂蜡工人的多聚酶调节","authors":"Norimitsu Izawa, Susumu Hiragaki, Amr A. Mohamed, Azza M. Elgendy, Takeshi Ohtani, Makio Takeda","doi":"10.1007/s13592-023-01015-x","DOIUrl":null,"url":null,"abstract":"<div><p>Arylalkylamine<i> N</i>-acetyltransferase (AANAT) is a large family of enzymes that catalyze the acetyl-CoA-dependent acetylation of an amine or arylalkylamine. Some AANATs, such as serotonin <i>N</i>-acetyltransferase, in vertebrates are photosensitive and mediate circadian regulation of melatonin and are involved in important biological processes. We analyzed AANAT activity of the honey bee, <i>Apis mellifera</i>, and found a relationship to the polyethism of workers. Crude AANAT solutions from isolated heads had optimum pHs at 8.0 (a minor peak) and 10.0 (a major peak). The Lineweaver–Burk (or double reciprocal plot) and the secondary plot (derived from the Lineweaver–Burk), gave the K<sub>m</sub> for tryptamine (substrate) as 2.65 µM, and the V<sub>max</sub> of 53.2 nmol/mg protein/min. The apparent K<sub>m</sub> and V<sub>max</sub> for acetyl-CoA was 20.5 µM and 6.98 nmol/mg protein/min, respectively. AANAT activity showed a correlation with the age of workers. The highest AANAT activity was found in 42-day old workers and the lowest in 3-day old workers. The highest AANAT activity was found in foragers and the lowest in hive-making workers. Two AANAT genes were retrieved from the <i>A. mellifera</i> genome. Putative <i>cis</i>-acting elements involved in circadian regulation, metamorphic and caste related expression were explored within both genes to discuss potential implication of the circadian clock in regulation of polyethism. The results suggest that AANATs are associated with regulation of social behavior in honey bees in circadian manners, in both foraging rhythms and polyethism.\n</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Arylalkylamine N-acetyltransferase activity parallel to work types and their temporal shift suggests its involvement in polyethism regulation in Apis mellifera workers\",\"authors\":\"Norimitsu Izawa, Susumu Hiragaki, Amr A. Mohamed, Azza M. Elgendy, Takeshi Ohtani, Makio Takeda\",\"doi\":\"10.1007/s13592-023-01015-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Arylalkylamine<i> N</i>-acetyltransferase (AANAT) is a large family of enzymes that catalyze the acetyl-CoA-dependent acetylation of an amine or arylalkylamine. Some AANATs, such as serotonin <i>N</i>-acetyltransferase, in vertebrates are photosensitive and mediate circadian regulation of melatonin and are involved in important biological processes. We analyzed AANAT activity of the honey bee, <i>Apis mellifera</i>, and found a relationship to the polyethism of workers. Crude AANAT solutions from isolated heads had optimum pHs at 8.0 (a minor peak) and 10.0 (a major peak). The Lineweaver–Burk (or double reciprocal plot) and the secondary plot (derived from the Lineweaver–Burk), gave the K<sub>m</sub> for tryptamine (substrate) as 2.65 µM, and the V<sub>max</sub> of 53.2 nmol/mg protein/min. The apparent K<sub>m</sub> and V<sub>max</sub> for acetyl-CoA was 20.5 µM and 6.98 nmol/mg protein/min, respectively. AANAT activity showed a correlation with the age of workers. The highest AANAT activity was found in 42-day old workers and the lowest in 3-day old workers. The highest AANAT activity was found in foragers and the lowest in hive-making workers. Two AANAT genes were retrieved from the <i>A. mellifera</i> genome. Putative <i>cis</i>-acting elements involved in circadian regulation, metamorphic and caste related expression were explored within both genes to discuss potential implication of the circadian clock in regulation of polyethism. The results suggest that AANATs are associated with regulation of social behavior in honey bees in circadian manners, in both foraging rhythms and polyethism.\\n</p></div>\",\"PeriodicalId\":8078,\"journal\":{\"name\":\"Apidologie\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2023-07-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Apidologie\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13592-023-01015-x\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Apidologie","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s13592-023-01015-x","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Arylalkylamine N-acetyltransferase activity parallel to work types and their temporal shift suggests its involvement in polyethism regulation in Apis mellifera workers
Arylalkylamine N-acetyltransferase (AANAT) is a large family of enzymes that catalyze the acetyl-CoA-dependent acetylation of an amine or arylalkylamine. Some AANATs, such as serotonin N-acetyltransferase, in vertebrates are photosensitive and mediate circadian regulation of melatonin and are involved in important biological processes. We analyzed AANAT activity of the honey bee, Apis mellifera, and found a relationship to the polyethism of workers. Crude AANAT solutions from isolated heads had optimum pHs at 8.0 (a minor peak) and 10.0 (a major peak). The Lineweaver–Burk (or double reciprocal plot) and the secondary plot (derived from the Lineweaver–Burk), gave the Km for tryptamine (substrate) as 2.65 µM, and the Vmax of 53.2 nmol/mg protein/min. The apparent Km and Vmax for acetyl-CoA was 20.5 µM and 6.98 nmol/mg protein/min, respectively. AANAT activity showed a correlation with the age of workers. The highest AANAT activity was found in 42-day old workers and the lowest in 3-day old workers. The highest AANAT activity was found in foragers and the lowest in hive-making workers. Two AANAT genes were retrieved from the A. mellifera genome. Putative cis-acting elements involved in circadian regulation, metamorphic and caste related expression were explored within both genes to discuss potential implication of the circadian clock in regulation of polyethism. The results suggest that AANATs are associated with regulation of social behavior in honey bees in circadian manners, in both foraging rhythms and polyethism.
期刊介绍:
Apidologie is a peer-reviewed journal devoted to the biology of insects belonging to the superfamily Apoidea.
Its range of coverage includes behavior, ecology, pollination, genetics, physiology, systematics, toxicology and pathology. Also accepted are papers on the rearing, exploitation and practical use of Apoidea and their products, as far as they make a clear contribution to the understanding of bee biology.
Apidologie is an official publication of the Institut National de la Recherche Agronomique (INRA) and Deutscher Imkerbund E.V. (D.I.B.)