Marta Klak , Katarzyna Kosowska , Tomasz Bryniarski , Ilona Łojszczyk , Tomasz Dobrzański , Grzegorz Tymicki , Anna Filip , Andrzej Antoni Szczepankiewicz , Radosław Olkowski , Anna Kosowska , Andrzej Berman , Artur Kamiński , Michał Wszoła
{"title":"基于dECM的Bioink用于仿生组织的3D生物打印，这是在小鼠模型上获得的第一个结果","authors":"Marta Klak , Katarzyna Kosowska , Tomasz Bryniarski , Ilona Łojszczyk , Tomasz Dobrzański , Grzegorz Tymicki , Anna Filip , Andrzej Antoni Szczepankiewicz , Radosław Olkowski , Anna Kosowska , Andrzej Berman , Artur Kamiński , Michał Wszoła","doi":"10.1016/j.bprint.2022.e00233","DOIUrl":null,"url":null,"abstract":"<div><p>Tissue engineering is an intensively developing field of modern medicine. However, to transfer the research from the laboratory scale to the clinic, it is necessary to produce biocompatible bioinks, that will be safe for both patients and cells used in the bioprinting process itself.</p><p>The aim of this researches was to produce a bioink that would enable bioprinting of structures with biological material, improving their functionality by recreating their natural environment.</p><p><span>The results showed the appropriate physicochemical properties of the biomaterial, which is stable </span><em>in vitro</em> and in vivo. <em>In vitro</em><span><span> studies showed the highest functionality of islets (7 days) in the bioprinted cell-printed constructs. The cytotoxicity tests showed the cell viability at the level of 120%. Moreover, the paper presents the first in vivo results for biodegradation of </span>bionic cell-printed constructs. During these studies, no inflammatory reaction to the implanted cell-printed construct was found for 12 months. The appearance of the first neovascular processes was shown in the 8th week of the experiment. At that time, there were no deviations in the level of blood parameters: AST,ALT,KC, IL-6 and TNF-α.</span></p><p>Summing up, the created bioink composition is safe for living organisms and contributes to the improvement of the functionality of pancreatic islets.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00233"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Bioink based on the dECM for 3D bioprinting of bionic tissue, the first results obtained on murine model\",\"authors\":\"Marta Klak , Katarzyna Kosowska , Tomasz Bryniarski , Ilona Łojszczyk , Tomasz Dobrzański , Grzegorz Tymicki , Anna Filip , Andrzej Antoni Szczepankiewicz , Radosław Olkowski , Anna Kosowska , Andrzej Berman , Artur Kamiński , Michał Wszoła\",\"doi\":\"10.1016/j.bprint.2022.e00233\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Tissue engineering is an intensively developing field of modern medicine. However, to transfer the research from the laboratory scale to the clinic, it is necessary to produce biocompatible bioinks, that will be safe for both patients and cells used in the bioprinting process itself.</p><p>The aim of this researches was to produce a bioink that would enable bioprinting of structures with biological material, improving their functionality by recreating their natural environment.</p><p><span>The results showed the appropriate physicochemical properties of the biomaterial, which is stable </span><em>in vitro</em> and in vivo. <em>In vitro</em><span><span> studies showed the highest functionality of islets (7 days) in the bioprinted cell-printed constructs. The cytotoxicity tests showed the cell viability at the level of 120%. Moreover, the paper presents the first in vivo results for biodegradation of </span>bionic cell-printed constructs. During these studies, no inflammatory reaction to the implanted cell-printed construct was found for 12 months. The appearance of the first neovascular processes was shown in the 8th week of the experiment. At that time, there were no deviations in the level of blood parameters: AST,ALT,KC, IL-6 and TNF-α.</span></p><p>Summing up, the created bioink composition is safe for living organisms and contributes to the improvement of the functionality of pancreatic islets.</p></div>\",\"PeriodicalId\":37770,\"journal\":{\"name\":\"Bioprinting\",\"volume\":\"28 \",\"pages\":\"Article e00233\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioprinting\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405886622000434\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Computer Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886622000434","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
Bioink based on the dECM for 3D bioprinting of bionic tissue, the first results obtained on murine model
Tissue engineering is an intensively developing field of modern medicine. However, to transfer the research from the laboratory scale to the clinic, it is necessary to produce biocompatible bioinks, that will be safe for both patients and cells used in the bioprinting process itself.
The aim of this researches was to produce a bioink that would enable bioprinting of structures with biological material, improving their functionality by recreating their natural environment.
The results showed the appropriate physicochemical properties of the biomaterial, which is stable in vitro and in vivo. In vitro studies showed the highest functionality of islets (7 days) in the bioprinted cell-printed constructs. The cytotoxicity tests showed the cell viability at the level of 120%. Moreover, the paper presents the first in vivo results for biodegradation of bionic cell-printed constructs. During these studies, no inflammatory reaction to the implanted cell-printed construct was found for 12 months. The appearance of the first neovascular processes was shown in the 8th week of the experiment. At that time, there were no deviations in the level of blood parameters: AST,ALT,KC, IL-6 and TNF-α.
Summing up, the created bioink composition is safe for living organisms and contributes to the improvement of the functionality of pancreatic islets.
期刊介绍：
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.
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