{"title":"玻璃离子释放对Y2O3/CeO2掺杂SiO2-SrO-Na2O玻璃细胞相容性、抗菌性能和抗氧化活性的影响","authors":"L. Placek, T. Keenan, A. Coughlan, A. Wren","doi":"10.1515/bglass-2018-0004","DOIUrl":null,"url":null,"abstract":"Abstract The effect on ion release and cytocompatibility of Yttrium (Y) and Cerium (Ce) are investigated when substituted for Sodium (Na) in a 0.52SiO2-0.24SrO-0.24-Na2OMOglass series (where MO= Y2O3 or CeO2). Glass leaching was evaluated through pH measurements and Inductive Coupled Plasma-Optical Emission Spectrometry (ICP-OES) analysiswhere the extract pH increased during incubation (11.2 - 12.5). Ion release of Silicon (Si), Na and Strontium (Sr) from the Con glass was at higher than that of glasses containing Y or Ce, and reached a limit after 1 day. Ion release from Y and Ce containing glasses reached a maximum of 1800 μg/mL, 1800 μg/mL, and 10 μg/mL for Si, Na, and Sr, respectively. Release of Y and Cewas below the ICP- OES detection limit <0.1 μg/mL. Cell viability of both L929 fibroblasts and MC3T3 osteoblasts decreased for Con, LY, and LCe extracts; HY extracts did not significantly decrease cell viability while YCe and HCe saw concentrationdependent viability decrease (20%, 33% extract concentrations). Bacterial studies saw Con and LCe eliminating >75% of bacteria at a 9% extract concentration. Antioxidant capacity (mechanism for neuroprotection) was evaluated using the ABTS assay. All glasses had inherent radical oxygen species (ROS) scavenging capability with Con reaching 9.5 mMTE.","PeriodicalId":37354,"journal":{"name":"Biomedical Glasses","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bglass-2018-0004","citationCount":"11","resultStr":"{\"title\":\"Investigating the Effect of Glass Ion Release on the Cytocompatibility, Antibacterial Eflcacy and Antioxidant Activity of Y2O3 / CeO2 doped SiO2-SrO-Na2O glasses\",\"authors\":\"L. Placek, T. Keenan, A. Coughlan, A. Wren\",\"doi\":\"10.1515/bglass-2018-0004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract The effect on ion release and cytocompatibility of Yttrium (Y) and Cerium (Ce) are investigated when substituted for Sodium (Na) in a 0.52SiO2-0.24SrO-0.24-Na2OMOglass series (where MO= Y2O3 or CeO2). Glass leaching was evaluated through pH measurements and Inductive Coupled Plasma-Optical Emission Spectrometry (ICP-OES) analysiswhere the extract pH increased during incubation (11.2 - 12.5). Ion release of Silicon (Si), Na and Strontium (Sr) from the Con glass was at higher than that of glasses containing Y or Ce, and reached a limit after 1 day. Ion release from Y and Ce containing glasses reached a maximum of 1800 μg/mL, 1800 μg/mL, and 10 μg/mL for Si, Na, and Sr, respectively. Release of Y and Cewas below the ICP- OES detection limit <0.1 μg/mL. Cell viability of both L929 fibroblasts and MC3T3 osteoblasts decreased for Con, LY, and LCe extracts; HY extracts did not significantly decrease cell viability while YCe and HCe saw concentrationdependent viability decrease (20%, 33% extract concentrations). Bacterial studies saw Con and LCe eliminating >75% of bacteria at a 9% extract concentration. Antioxidant capacity (mechanism for neuroprotection) was evaluated using the ABTS assay. All glasses had inherent radical oxygen species (ROS) scavenging capability with Con reaching 9.5 mMTE.\",\"PeriodicalId\":37354,\"journal\":{\"name\":\"Biomedical Glasses\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-02-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1515/bglass-2018-0004\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Glasses\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/bglass-2018-0004\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Materials Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Glasses","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/bglass-2018-0004","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Materials Science","Score":null,"Total":0}
Investigating the Effect of Glass Ion Release on the Cytocompatibility, Antibacterial Eflcacy and Antioxidant Activity of Y2O3 / CeO2 doped SiO2-SrO-Na2O glasses
Abstract The effect on ion release and cytocompatibility of Yttrium (Y) and Cerium (Ce) are investigated when substituted for Sodium (Na) in a 0.52SiO2-0.24SrO-0.24-Na2OMOglass series (where MO= Y2O3 or CeO2). Glass leaching was evaluated through pH measurements and Inductive Coupled Plasma-Optical Emission Spectrometry (ICP-OES) analysiswhere the extract pH increased during incubation (11.2 - 12.5). Ion release of Silicon (Si), Na and Strontium (Sr) from the Con glass was at higher than that of glasses containing Y or Ce, and reached a limit after 1 day. Ion release from Y and Ce containing glasses reached a maximum of 1800 μg/mL, 1800 μg/mL, and 10 μg/mL for Si, Na, and Sr, respectively. Release of Y and Cewas below the ICP- OES detection limit <0.1 μg/mL. Cell viability of both L929 fibroblasts and MC3T3 osteoblasts decreased for Con, LY, and LCe extracts; HY extracts did not significantly decrease cell viability while YCe and HCe saw concentrationdependent viability decrease (20%, 33% extract concentrations). Bacterial studies saw Con and LCe eliminating >75% of bacteria at a 9% extract concentration. Antioxidant capacity (mechanism for neuroprotection) was evaluated using the ABTS assay. All glasses had inherent radical oxygen species (ROS) scavenging capability with Con reaching 9.5 mMTE.
期刊介绍:
Biomedical Glasses is an international Open Access-only journal covering the field of glasses for biomedical applications. The scope of the journal covers the science and technology of glasses and glass-based materials intended for applications in medicine and dentistry. It includes: Chemistry, physics, structure, design and characterization of biomedical glasses Surface science and interactions of biomedical glasses with aqueous and biological media Modeling structure and reactivity of biomedical glasses and their interfaces Biocompatibility of biomedical glasses Processing of biomedical glasses to achieve specific forms and functionality Biomedical glass coatings and composites In vitro and in vivo evaluation of biomedical glasses Glasses and glass-ceramics in engineered regeneration of tissues and organs Glass-based devices for medical and dental applications Application of glasses and glass-ceramics in healthcare.