通过转录组和基因组分析鉴定C2H2基因参与低温调控缓解桃果采后冷害

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Quality and Safety Pub Date : 2022-10-12 DOI:10.1093/fqsafe/fyac059

Yaqin Zheng, Z. Liu, Hui Wang, Wenna Zhang, Shuaijie Li, Meng Xu

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引用次数: 3

摘要

本研究旨在确定参与低温调节(LTC)缓解桃果采后冷害的候选C2H2基因。在低温贮藏条件下，将果实在10℃下预贮藏5 d，然后转入0℃贮藏。用硬度计测定果实硬度。利用多功能酶标机光度测量模型测定H2O2含量。利用HMMSCAN对桃基因组进行C2H2家族成员的鉴定。利用Plant CARE网站分析基因启动子的顺式作用元件。采用BMK Cloud平台的WGCNA包进行WGCNA分析。LTC处理降低了北京9号桃果肉褐变率和H2O2产量。0℃和LTC处理下的DEGs转录因子鉴定表明，C2H2参与了桃冷害的调控。基于桃基因组共鉴定出47个C2H2基因。qRT-PCR、系统发育分析和启动子顺式作用元件分析表明，ZFP21参与了ltc介导的桃低温损伤的调控。加权基因共表达网络分析和双荧光素酶实验表明，ZFP21通过下调ros相关基因Rboh的表达参与了ltc减轻的低温损伤。基于基因组和RNA-seq的研究表明，ZFP21参与了LTC处理减轻桃果实冷害的过程。本研究对桃耐冷相关基因的鉴定和桃C2H2家族的研究具有一定的指导意义。

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Transcriptome and genome analysis to identify C2H2 genes participated in Low temperature conditioning-alleviated postharvest chilling injury of peach fruit

The work intended to identify candidate C2H2 genes participated in Low-temperature conditioning (LTC) -alleviated postharvest chilling injury of peach fruit. For LTC treatment, fruit were pre-stored at 10 oC for 5 d, and then transferred to 0 oC storage. Fruit firmness was measured by the hardness tester. H2O2 content was determined by luminosity measurement model using the multifunctional enzyme labeler. Identification of C2H2 family members was performed by HMMSCAN according to peach genome. Cis-acting element of gene promoters was analyzed using the Plant CARE website. WGCNA analysis was performed by WGCNA package in BMK Cloud platform. LTC treatment decreased flesh browning rate and H2O2 production of ‘Beijing No.9’ peach. Transcription factors identification of DEGs in 0 oC and LTC treatment indicated peach C2H2 participated the regulation of chilling injury. A total of 47 C2H2 genes were identified based on peach genome. qRT-PCR, phylogenetic analysis and promoter cis-acting element analysis revealed ZFP21 was involved in the regulation of LTC-alleviated chilling injury in peach. Weighted gene co-expression network analysis and dual luciferase assay suggested ZFP21 participated in LTC-alleviated chilling injury by down-regulating the expression of ROS-related genes Rboh. Our investigation, based on genome and RNA-seq, unveiled that ZFP21 was involved in LTC treatment-alleviated chilling injury of peach fruit. This work is useful for the identification of peach cold tolerance-related genes and the study of C2H2 family in peach.

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来源期刊

Food Quality and Safety FOOD SCIENCE & TECHNOLOGY-

CiteScore

7.20

自引率

1.80%

发文量

审稿时长

5 weeks

期刊介绍： Food quality and safety are the main targets of investigation in food production. Therefore, reliable paths to detect, identify, quantify, characterize and monitor quality and safety issues occurring in food are of great interest. Food Quality and Safety is an open access, international, peer-reviewed journal providing a platform to highlight emerging and innovative science and technology in the agro-food field, publishing up-to-date research in the areas of food quality and safety, food nutrition and human health. It promotes food and health equity which will consequently promote public health and combat diseases. The journal is an effective channel of communication between food scientists, nutritionists, public health professionals, food producers, food marketers, policy makers, governmental and non-governmental agencies, and others concerned with the food safety, nutrition and public health dimensions. The journal accepts original research articles, review papers, technical reports, case studies, conference reports, and book reviews articles.