用间接免疫荧光法观察拉丁美洲某三级卫生中心患者的抗核抗体染色模式

Q3 Health Professions

Revista Colombiana de Reumatologia Pub Date : 2024-07-01 DOI:10.1016/j.rcreu.2023.05.003

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引用次数: 0

摘要

导言/目的本研究旨在描述拉丁美洲一家三级医疗中心的患者通过间接免疫荧光测定观察到的抗核抗体（ANA）染色模式的频率。材料和方法这项回顾性、描述性和观察性研究评估了 2020 年在一家三级医疗中心（哥伦比亚卡利市 Valle del Lili 基金会大学医院）接受抗核抗体间接免疫荧光检测的所有患者的数据。患者年龄中位数为 47（34-59.2）岁，大多数为女性（769 人，75.3%）。约三分之二的患者（664 人，65.8%）ANA 免疫荧光检测呈阳性。约半数患者（466 人，46.2%）的 ANA 检测结果主要用于排除疑似诊断。在 ANA 滴度呈阳性的人群中，37%（250/664）的患者患有系统性自身免疫性风湿病 (SARD)。最常见的系统性自身免疫性风湿病包括类风湿性关节炎（RA）（55 例，占 8.2%），其次是系统性红斑狼疮（SLE）（37 例，占 5.5%）。据报道，绝大多数 ANA 阳性患者有斑点模式（抗细胞[AC]-2,4,5；269；40.5%），其次是均质模式（AC-1；266；40%）、核仁模式（AC-8,9,10；46；6.9%）和中心粒模式（AC-3；16；2.4%）。在系统性红斑狼疮患者中观察到的最常见模式包括：17 名患者（45.9%）的均质（AC-1）模式、11 名患者（29.7%）的斑点（AC-2,4,5）核模式、7 名患者（18.9%）的混合模式，以及 2 名患者（5.4%）的网状/抗线粒体抗体（AMA，AC-21）细胞质模式。在 SARDs 患者中，斑点和均质模式占主导地位。

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Antinuclear antibody staining patterns by indirect immunofluorescence assay observed in patients from a tertiary health center in Latin America

Introduction/Objective

This study aimed to describe the frequency of antinuclear antibody (ANA) staining patterns by indirect immunofluorescence assay observed in patients from a tertiary health center in Latin America.

Materials and methods

This retrospective, descriptive, and observational study evaluated data from all patients undergoing antinuclear antibody indirect immunofluorescence assay from a single-tertiary center (University Hospital Fundación Valle del Lili, Cali-Colombia) in 2020.

Results

One thousand and eight patients met the inclusion criteria. The median patient age was 47 (34–59.2) years, and most were female (769, 75.3%). A positive ANA immunofluorescence assay was observed in approximately two-thirds of patients (664, 65.8%). ANA test results were primarily used to exclude a suspected diagnosis in approximately half of the patients (466, 46.2%). Thirty-seven percent (250/664) of the cohort with ANA-positive titers had a systemic autoimmune rheumatic disease (SARD). The most prevalent SARDs included rheumatoid arthritis (RA) (55, 8.2%) followed by systemic lupus erythematosus (SLE) (37, 5.5%). The vast majority of ANA-positive patients had a reported speckled pattern (anti-cell [AC]-2,4,5; 269; 40.5%) followed by homogenous (AC-1; 266; 40%), nucleolar (AC-8,9,10; 46; 6.9%), and centromere (AC-3; 16; 2.4%). The most frequent patterns observed among SLE patients included homogenous (AC-1) patterns in 17 (45.9%) patients, speckled (AC-2,4,5) nuclear patterns in 11 (29.7%) patients, mixed patterns in 7 (18.9%) patients, and reticular/anti-mitochondrial antibody (AMA, AC-21) cytoplasmic patterns in 2 (5.4%) patients.

Conclusion

This study is the first to describe ANA patterns in a Colombian population. Speckled and homogenous patterns were predominant in patients with SARDs.

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来源期刊

Revista Colombiana de Reumatologia Medicine-Rheumatology

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： The Colombian Journal of Rheumatology (Revista Colombiana de Reumatología) is the official organ of the Colombian Association of Rheumatology (Asociación Colombiana de Reumatología) and the Central American, Caribbean and Andean Association of Rheumatology (Asociación Centroamericana Caribe Andina de Reumatología) - ACCA. It was created in December 1993 with the purpose of disseminating scientific information derived from primary and secondary research and presenting cases coming from the practice of Rheumatology in Latin America. Since its foundation, the Journal has been characterized by its plurality with subjects of all rheumatic and osteomuscular pathologies, in the form of original articles, historical articles, economic evaluations, and articles of reflection and education in Medicine. It covers an extensive area of topics ranging from the broad spectrum of the clinical aspects of rheumatology and related areas in autoimmunity (both in pediatric and adult pathologies), to aspects of basic sciences. It is an academic tool for the different members of the academic and scientific community at their different levels of training, from undergraduate to post-doctoral degrees, managing to integrate all actors inter and trans disciplinarily. It is intended for rheumatologists, general internists, specialists in related areas, and general practitioners in the country and abroad. It has become an important space in the work of all rheumatologists from Central and South America.