养殖北方盾鱼(Silurus soldatovi)肠道微生物群的变化与自然细菌感染有关

IF 2.3 3区 农林科学 Q2 FISHERIES
Zhongjie Zan, Qing Mao, Zhuoran Han, Jingfeng Sun
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引用次数: 0

摘要

天津某商业养殖池中发现一种北方盾鱼(Silurus soldatovi)急性传染病。从自然患病的北棘鱼中分离到两株优势菌株,鉴定为维罗氏气单胞菌和霍乱弧菌。采用高通量16S rRNA基因测序法对该鱼塘健康鱼和自然病鱼各7条进行肠道菌群分析。α-多样性指数(ACE、Chao 1和Shannon)在病鱼和健康鱼之间无显著差异(p > 0.05),但在对细菌群体进行β-多样性分析时发现两者之间存在显著差异。与健康鱼相比,患病鱼的气单胞菌、弧菌、蓝单胞菌和巨单胞菌的相对丰度显著升高,而鲸杆菌、拟杆菌、黄绿杆菌、拉尔斯顿菌和假单胞菌的相对丰度则显著降低(p < 0.05)。线性判别分析效应大小显示,病鱼的微生物标记种为气单胞菌目、气单胞菌科、气单胞菌属。利用PICRUSt2预测的微生物群落功能谱表明,肠道微生物群的紊乱导致病鱼肠道微生物群功能谱的改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Changes in the intestinal microbiota of farmed northern sheatfish (Silurus soldatovi) associated with natural bacterial infection

Changes in the intestinal microbiota of farmed northern sheatfish (Silurus soldatovi) associated with natural bacterial infection

An acute infectious disease of northern sheatfish (Silurus soldatovi) was found in a commercial aquaculture pond in Tianjin, China. Herein, two dominant bacterial strains were isolated from naturally diseased northern sheatfish and identified as Aeromonas veronii and Vibrio cholerae. Seven healthy and seven naturally diseased fish that were collected from the pond were used for the gut microbiota analysis by high-throughput 16S rRNA gene sequencing. The α-diversity indices (ACE, Chao 1, and Shannon) did not differ significantly between diseased and healthy fish (p > 0.05), but a significant separation was observed between them when β-diversity analysis of the bacterial population was performed. The diseased fish demonstrated a significant elevation in the relative abundances of Aeromonas, Vibrio, Blautia, and Megamonas and a decrease in Cetobacterium, Bacteroides, Chryseolinea, Ralstonia, and Pseudomonas compared with healthy fish (p < 0.05). Linear discriminant analysis effect size showed that microbial marker species in the diseased fish were in the order Aeromonadales, the family Aeromonadaceae, and the genus Aeromonas. The functional profiles of the microbial community that were predicted using the PICRUSt2 indicated that the disturbance of gut microbiota caused the alteration of its functional profiles in the diseased fish.

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来源期刊
CiteScore
5.90
自引率
7.10%
发文量
69
审稿时长
2 months
期刊介绍: The Journal of the World Aquaculture Society is an international scientific journal publishing original research on the culture of aquatic plants and animals including: Nutrition; Disease; Genetics and breeding; Physiology; Environmental quality; Culture systems engineering; Husbandry practices; Economics and marketing.
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