来自烟灰的R2R3 MYB转录因子正调控烟草对盐的反应

IF 1.1 4区生物学 Q3 BIOLOGY

Turkish Journal of Biology Pub Date : 2017-08-01 DOI:10.3906/biy-1612-31

Tian Li, Jingkuan Sun, Y. Bi

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引用次数: 0

摘要

MYB转录因子在调节植物环境胁迫中的作用是多样的和重要的。本研究从白蜡树（Fraxinus velutina Torr.）中克隆了R2R3 MYB基因FvMYB2（GenBank登录号KY767843）及其启动子区。PlantCARE软件分析表明，其启动子含有不同的顺式元素，参与不同的非生物胁迫。研究了FvMYB2在不同非生物胁迫条件下的表达模式。FvMYB2亚细胞定位主要定位于细胞核。NaCl处理后，烟草中FvMYB2的过表达增强了耐盐性，并上调了胁迫和ABA相关基因。这些结果表明FvMYB2可能在ABA依赖性信号传导机制介导的盐胁迫调节中发挥积极作用。

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A R2R3 MYB transcription factor from ash positively regulates salt response in tobacco

The roles of MYB transcription factors are diverse and important in regulating environmental stress in plants. In this study, the R2R3 MYB gene FvMYB2 (GenBank Accession No. KY767843) and its promoter region were cloned from ash (Fraxinus velutina Torr.). Phylogenetic analysis revealed that FvMYB2 was clustered with VvMYB60 from Vitis vinifera. PlantCARE software analysis showed that its promoter contained different cis elements involved in diverse abiotic stresses. The expression patterns of FvMYB2 were investigated under different abiotic stress conditions. FvMYB2 subcellular localization was mainly localized in the nucleus. Overexpression of FvMYB2 in tobacco showed enhanced salt resistance and upregulation of stress- and ABA-related genes after NaCl treatment. These results indicated that FvMYB2 may play a positive role in salt stress regulation mediated by ABA-dependent signaling mechanisms.

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来源期刊

Turkish Journal of Biology BIOLOGY-

CiteScore

4.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Turkish Journal of Biology is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts English-language manuscripts concerning all kinds of biological processes including biochemistry and biosynthesis, physiology and metabolism, molecular genetics, molecular biology, genomics, proteomics, molecular farming, biotechnology/genetic transformation, nanobiotechnology, bioinformatics and systems biology, cell and developmental biology, stem cell biology, and reproductive biology. Contribution is open to researchers of all nationalities.