人乳头瘤病毒在口咽鳞状细胞癌中的p16免疫组化检测

IF 0.8 Q3 MEDICINE, GENERAL & INTERNAL
D. Uppala, A. Sabu, NV Ratna Mouli, N. Tejaswini, V. Rohit, G. Nishitha
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引用次数: 6

摘要

简介:口腔癌症在印度所有癌症中排名第三,印度约45%的癌症病例被诊断为口腔癌症,其中20%-50%的病例被观察到与人乳头瘤病毒(HPV)感染有关。目的:本研究旨在利用免疫组织化学(IHC)检测和评估p16在口咽鳞状细胞癌(OPSCC)患者中的存在。材料和方法:本研究基于2017年12月至2018年3月期间诊断和治疗的21名原发性口腔鳞状细胞癌患者的样本。纳入标准是完整的临床病理数据、足够的临床随访和足够的石蜡包埋肿瘤材料的可用性。以p16作为标记物,通过IHC进一步研究HPV免疫反应性。结果:6例OPSCC患者的IHC结果显示p16阳性。p16阳性HPV与年龄、性别或部位无统计学意义的相关性。结论:我们的结果表明,如果不结合HPV DNA检测,基于IHC的p16检测提供了次优的预后信息。尽管p16表达和HPV DNA感染与HPV相关的OPSCCs相关,但单独的两种测试都不是检测HPV状态的最佳方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human Papillomavirus Detection in Oropharyngeal Squamous Cell Carcinoma Using p16 Immunohistochemistry
Introduction: Oral cancer ranks third among all cancers in the Indian population with approximately 45% of call cancer cases in India being diagnosed as oral cancer, among which 20%–50% of the cases are observed to be associated with human papillomavirus (HPV) infection. Aim: This study aims to detect and evaluate the presence of p16 in oropharyngeal squamous cell carcinoma (OPSCC) patients using immunohistochemistry (IHC). Materials and Methods: This study was based on samples collected from 21 patients with primary OPSCC who were diagnosed and treated during the period of December 2017–March 2018. Inclusion criteria were complete clinicopathologic data, adequate clinical follow-up, and availability of sufficient paraffin-embedded tumor material. HPV immunoreactivity was further investigated by means of IHC using p16 as a marker. Results: IHC results revealed p16 positivity in six OPSCC cases. There was no statistically significant association of the p16 positivity of HPV with the age, gender, or site. Conclusion: Our results suggest that IHC-based detection of p16 provides a suboptimal prognostic information if not combined with detection of HPV DNA. Although p16 expression and HPV DNA infection are correlated with HPV-associated OPSCCs, neither of the tests alone is the optimal method for HPV status detection.
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