细胞片工程的临床翻译:技术方面

Q1 Engineering
Irina M. Zurina , Victoria S. Presniakova , Denis V. Butnaru , Peter S. Timashev , Yury A. Rochev , Xing-Jie Liang
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引用次数: 2

摘要

细胞片工程是组织工程和再生医学的一个快速发展的领域。细胞片获取技术的便利性以及由此产生的这些多细胞结构的独特特性和微环境使其在体内的应用范围广泛。同时,也有宏观尺度的细胞片性能,如厚度、因细胞骨架松弛而脱离后的收缩,以及由此产生的力学特性。本综述的主要主题是讨论这些特性,以及它们如何定义在堆叠多个结构、将其转移到手术部位或冷冻保存期间使用特殊方法操纵细胞片的必要性。我们的目的是系统化现有的细胞片转移技术,并描述其原理,优点,以及在各种组织和器官的外科手术中应用细胞片的缺点。我们还关注了一些方面和细节,如细胞片在体内的定位,它们自发粘附的能力,以及在特定手术部位额外固定的要求。最后,本综述的最后一部分涵盖了细胞片冷冻保存的主题-冷冻和解冻方案的讨论,各种冷冻保护剂及其混合物,以及特殊要求,如冷冻保护剂加载系统和细胞片支撑系统,也源于其独特的宏观特征。总之,对现有的与细胞片在体内应用相关的技术方法进行系统的回顾,可能对组织工程领域的新专家和专家研究人员有潜在的帮助。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Towards clinical translation of the cell sheet engineering: Technological aspects

Cell sheet engineering is a rapidly growing field of tissue engineering and regenerative medicine. The ease of cell sheet obtainment techniques and the resulting unique characteristics and microenvironment of these multicellular structures give rise to the wide range of their in vivo application. At the same time, there are also macroscale cell sheet properties such as thickness, shrinkage after detachment due to cytoskeleton relaxation, and resulting mechanical characteristics. The main topic of this review is the discussion of these properties and how they define the need to use special approaches to manipulating cell sheets during stacking several structures, transferring them to surgical sites, or cryopreserving them. We aimed to systematize the existing techniques of cell sheet transferring, and describe their principles, advantages, and drawbacks regarding cell sheet application during surgical procedures on various tissues and organs. Attention is also paid to such aspects and details as cell sheet positioning in vivo, their ability to spontaneous adhesion, and the requirement for additional fixation at particular surgical sites. Finally, the last section of this review covers the subject of cell sheet cryopreservation – the discussion of freezing and thawing protocols, the variety of cryoprotectants and their mixtures, as well as special requirements such as cryoprotectant loading systems, and cell sheet supporting systems that also stem from their unique macroscale characteristics. Altogether, this systematized review of existing technological approaches related to cell sheet application in vivo can be potentially helpful for the new and expert researchers in this area of tissue engineering.

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来源期刊
Smart Materials in Medicine
Smart Materials in Medicine Engineering-Biomedical Engineering
CiteScore
14.00
自引率
0.00%
发文量
41
审稿时长
48 days
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