维生素d3 -他莫昔芬联合微乳对McF-7乳腺细胞株的抗癌活性及其协同作用

Q4 Pharmacology, Toxicology and Pharmaceutics

INDIAN DRUGS Pub Date : 2023-08-28 DOI:10.53879/id.60.08.14101

Krantisagar S. More, B. Dalal, Aruna Shankarkumar, P. Devarajan

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引用次数: 1

摘要

本研究探讨了维生素D3和他莫昔芬(TMX-VD3 ME)联合微乳对MCF-7乳腺癌细胞系的协同作用。将药物溶解于油中，与表面活性剂、助表面活性剂和水混合，制备复合微乳液。对ME (VD3 1.5 mg mL-1, TMX 10 mg mL-1)的稳定性、粒径和PDI进行了优化。采用MTT法评价微乳的细胞毒性。结果表明，浓度依赖性增加细胞摄取与降低细胞活力。流式细胞术显示，G0/G1期细胞凋亡和细胞周期阻滞增强，迁移实验的效率提高了3.45倍。此外，与单独使用维生素D3 ME或他莫昔芬ME相比，联合使用TMX-VD3 ME微乳的抗癌效果更强，表明两者具有协同作用。斑马鱼模型显示维生素D3 ME制剂具有协同抗血管生成活性。

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ANTICANCER ACTIVITY OF VITAMIN D3–TAMOXIFEN COMBINATION MICROEMULSION ON MCF-7 BREAST CELL LINE AND ITS SYNERGISTIC EFFECT

This study investigates the anticancer activity of a combination microemulsion of vitamin D3 and tamoxifen (TMX-VD3 ME) for a synergistic effect on the MCF-7 breast cancer cell line. The combination microemulsion was prepared by dissolving the drugs in oil, mixing the oil with a surfactant, cosurfactant, and water. ME (VD3 1.5 mg mL-1, TMX 10 mg mL-1) was optimized for stability, globule size, and PDI. MTT assay was used to evaluate the cytotoxicity of the microemulsion. The results demonstrated a concentration-dependent increase in cell uptake with a decrease in cell viability. Flow cytometry revealed enhanced apoptosis and cell cycle arrest in G0/G1 phase and 3.45-fold enhanced efficacy in the migration assay. Additionally, the combination TMX-VD3 ME microemulsion exhibited enhanced anticancer efficacy compared to individual treatments of vitamin D3 ME or tamoxifen ME alone, indicating a synergistic effect. The zebrafish model revealed synergistic antiangiogenic activity of the vitamin D3 ME formulations.

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来源期刊

INDIAN DRUGS Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.30

自引率

0.00%

发文量