LC-MS /MS同时测定大鼠血浆中利伐沙班和西格列汀的含量及其在药物相互作用研究中的应用

IF 1.7 4区化学 Q3 CHEMISTRY, ANALYTICAL

Acta Chromatographica Pub Date : 2021-12-28 DOI:10.1556/1326.2021.00988

Libin Wang, Kun Shang, Xiaorui Zhang, Xin-Yuan Zhang, Tian Feng, Xu Xiaohui, Fang Wang

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引用次数: 2

摘要

建立了以阿哌沙班为内标(IS)同时定量大鼠血浆中利伐沙班(RIV)和西格列汀(SIT)的灵敏、准确的LC-MS/MS方法。采用Agilent Eclipse加C18色谱柱(2.1 × 100 mm, 3.5µm, Agilent)，等密度洗脱进行色谱分离。多反应监测(MRM)采用正离子ESI模式监测RIV的m/z 436.8→144.9、SIT的m/z 407.7→173.8、IS的m/z 459.8→442.8的离子跃迁。方法验证包括选择性、线性度、精密度、准确度、基质效应、提取回收率和稳定性，验证过程按照EMA和FDA的指导原则进行。结果表明，两种药物之间没有发生明显的相互作用，这可能是由于它们的代谢途径不同所致。

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Simultaneous determination of rivaroxaban and sitagliptin in rat plasma by LC–MS/MS and its application to pharmacokinetic drug-drug interaction study

A sensitive and accurate LC-MS/MS method was developed and validated for the simultaneous quantification of rivaroxaban (RIV) and sitagliptin (SIT) in rat plasma using apixaban as internal standard (IS). An Agilent Eclipse plus C18 column (2.1 × 100 mm, 3.5 µm, Agilent) was used for chromatographic separation with isocratic elution. Multiple reaction monitoring (MRM) using positive-ion ESI mode to monitor ion transitions of m/z 436.8→144.9 for RIV, m/z 407.7→173.8 for SIT, m/z 459.8→442.8 for IS. The procedure of method validation included selectivity, linearity, precision, accuracy, matrix effect, extraction recovery and stability were conducted according to the guidelines of EMA and FDA. The results indicated that no obvious drug-drug interactions occurred might be owing to their differences in metabolic pathways.

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来源期刊

Acta Chromatographica 化学-分析化学

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

2.3 months

期刊介绍： Acta Chromatographica Open Access Acta Chromatographica publishes peer-reviewed scientific articles on every field of chromatography, including theory of chromatography; progress in synthesis and characterization of new stationary phases; chromatography of organic, inorganic and complex compounds; enantioseparation and chromatography of chiral compounds; applications of chromatography in biology, pharmacy, medicine, and food analysis; environmental applications of chromatography; analytical and physico-chemical aspects of sample preparation for chromatography; hyphenated and combined techniques; chemometrics and its applications in separation science.