益生菌成分对实验性骨关节炎大鼠血液氧化/抗氧化平衡的影响

Q4 Biochemistry, Genetics and Molecular Biology
O. Korotkyi, K. Dvorshchenko, A. Vovk, A. Dranitsina, M. Tymoshenko, L. Kot, L. Ostapchenko
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引用次数: 12

摘要

骨关节炎(OA)是一种广泛的肌肉骨骼系统病理。OA可能与广泛的疾病有关,这导致了如何预防和治疗该疾病的各种战略的发展。最近的研究讨论了微生物组与包括OA在内的多种病理之间的相互作用。在本研究中,我们研究了益生菌培养物对OA大鼠血液中氧化/抗氧化平衡的影响。采用单碘乙酸钠单次注入膝关节诱导实验性骨关节炎。益生菌组合物(Multiprobiotic Simbiter®)通过经口导管给予,每天一次,持续14天。我们研究了血液中Nos2基因的表达、超氧化物歧化酶活性、过氧化氢酶活性、谷胱甘肽过氧化物酶活性、谷胱甘肽转移酶活性、谷胱甘肽还原酶活性、血清中超氧化物、过氧化氢、tba活性化合物、氧化谷胱甘肽和还原性谷胱甘肽的含量。单碘乙酸诱导的OA引起大鼠血液中氧化/抗氧化平衡的显著变化:超氧阴离子自由基、过氧化氢、硫代巴比妥酸活性化合物、氧化谷胱甘肽含量增加,Nos2表达上调,过氧化氢酶活性增加;相反,与对照组相比,超氧化物歧化酶活性、谷胱甘肽过氧化物活性、谷胱甘肽转移酶活性、谷胱甘肽还原酶活性、还原性谷胱甘肽含量显著降低。OA动物给予益生菌后,研究参数正向变化,接近对照组(部分变化有统计学意义)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of probiotic composition on oxidative/antioxidant balance in blood of rats under experimental osteoarthriti
Osteoarthritis (OA) is a widespread pathology of the musculoskeletal system. OA may associate with a wide range of disorders, that lead to the development of various strategies on how to prevent and treat the disease. recent studies discussed interactions between the microbiome and a wide range of pathologies, including OA. In this study, we investigated the effect of probiotic cultures on oxidative/antioxidant balance in blood of rats during OA. Experimental OA was induced by a single injection of sodium monoiodoacetate into the knee joint. A probiotic composition (Multiprobiotic Simbiter®) was administered by peroral catheter once per day for 14 days. We investigated the next parameters: expression of Nos2 gene in the blood, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione transferase activity, glutathione reductase activity, contents of superoxide, hydrogen peroxide, TBA-reactive compounds, oxidized and reduced glutathione in the serum of the blood. Monoiodoacetate-induced OA caused significant changes on oxidative/antioxidant balance in the blood of rats: increasing of the contents of superoxide anion radical, hydrogen peroxide, thiobarbituric acid-reactive compounds, oxidized glutathione, upregulating of Nos2 expression, increasing of catalase activity; conversely, superoxide dismutase activity, glutathione peroxidize activity, glutathione transferase activity, glutathione reductase activity, the content of reduced glutathione were significantly decreased, compared to control group. Administration of probiotics to animals with OA led to positive changes in the studied parameters approaching the values of control group (some of them were statistically significant).
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来源期刊
Ukrainian Biochemical Journal
Ukrainian Biochemical Journal Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
1.20
自引率
0.00%
发文量
37
审稿时长
16 weeks
期刊介绍: The Ukrainian Biochemical Journal publishes original research papers, reviews and brief notes; papers on research methods and techniques; articles on the history of biochemistry, its development and prominent figures; discussion articles; book reviews; chronicles; etc. The journal scope includes not only biochemistry but also related sciences, such as cellular and molecular biology, bioorganic chemistry, biophysics, pharmacology, genetics, and medicine (medical biochemistry et al.) – insofar as the studies use biochemical methods and discuss biochemical findings.
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