白藜芦醇可防止暴露于气态甲醛的大鼠脑损伤和神经颗粒蛋白表达

中华行为医学与脑科学杂志 Pub Date : 2020-02-20 DOI:10.3760/CMA.J.CN371468-20190916-00633

Li Peng, Xiang Zhang, Zhongming Li, Wei Fan, Xing Liu, Xiaohan Wei, Jie Deng

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The animal model of gaseous formaldehyde accumulation was established by gas formaldehyde exposure method.Learning and memory function was tested by Morris water maze. Cortical and hippocampal neurogranin (Ng) protein expression in brain cortex and hippocampus was measured by Western blot. \n \n \nResults \n(1) Compared with the escape latency and original platform retention time of rats in group N ((19.75±4.08) s, (43.47±2.43) s), the escape latency of rats in FM and FH groups was prolonged ((25.52±2.98) s, (27.45±2.39) s, t=-4.97, -5.82; both P<0.01), the retention time of the original platform was shortened ((30.99±4.21) s, (18.70±2.58) s, t=10.24, 25.71; both P<0.01). Compared with the corresponding formaldehyde accumulation groups such as the FM and FH groups, the escape latency of the rats in the FMR and FHR groups was significantly longer ((21.37±3.80) s, (24.26±3.56) s, t=1.86, 3.93; both P<0.05), the retention time of the original platform was significantly prolonged ((40.33±3.63)s, (23.46±4.73)s, t=-7.50, -3.95; both P<0.01). (2) Compared with the expression of Ng in cortex and hippocampus of rats in group N ((0.99±0.03), (1.07±0.03)), the expression of Ng protein in cortex and hippocampus of rats in FM and FH groups was significantly reduced((0.62±0.06), (0.39±0.03), (0.77±0.09), (0.46±0.10), t=11.55, 14.08, 11.51, 13.17; all P<0.01). 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引用次数: 0

摘要

目的探讨气态甲醛暴露对雄性Sprague-Dawley大鼠脑功能和突触蛋白表达的影响以及白藜芦醇的神经保护作用。方法84只雄性sd大鼠随机分为7组，每组12只:对照组(N)、气态甲醛积累低浓度组(FL组)、气态甲醛积累中浓度组(FM组)、气态甲醛积累高浓度组(FH组)、气态甲醛积累低浓度+白藜芦醇组(FLR组)、气态甲醛积累浓度+白藜芦醇组(FMR组)和气态甲醛积累高浓度+白藜芦醇组(FHR组)。采用气体甲醛暴露法建立气体甲醛积累动物模型。Morris水迷宫测试学习记忆功能。Western blot检测脑皮层和海马皮层神经颗粒蛋白(Ng)的表达。结果(1)与N组大鼠的逃逸潜伏期和原始平台滞留时间((19.75±4.08)s、(43.47±2.43)s相比，FM组和FH组大鼠的逃逸潜伏期延长((25.52±2.98)s、(27.45±2.39)s, t=-4.97、-5.82;P均<0.01)，原平台固位时间缩短((30.99±4.21)s，(18.70±2.58)s, t=10.24, 25.71;P < 0.01)。与FM和FH组等相应的甲醛积累组相比，FMR和FHR组大鼠的逃避潜伏期显著延长((21.37±3.80)s，(24.26±3.56)s, t=1.86, 3.93;均P<0.05)，原平台固位时间显著延长((40.33±3.63)s，(23.46±4.73)s, t=-7.50， -3.95;P < 0.01)。(2)与N组大鼠皮质和海马中Ng蛋白表达量((0.99±0.03)，(1.07±0.03)相比，FM和FH组大鼠皮质和海马中Ng蛋白表达量显著降低((0.62±0.06)，(0.39±0.03)，(0.77±0.09)，(0.46±0.10)，t=11.55, 14.08, 11.51, 13.17;所有P < 0.01)。与相应的甲醛积累组(FM和FH组)相比，FMR和FHR组大鼠皮质和海马中Ng的表达量显著升高((0.94±0.11)、(0.48±0.04)、(0.95±0.05)、(0.60±0.09)，t=-5.26、-2.09、-5.32、-2.21;所有P < 0.05)。结论白藜芦醇能改善气态甲醛积累所致大鼠的学习记忆功能。其机制可能与Ng的上调有关。关键词:气态甲醛积聚;Neurogranin;学习和记忆;白藜芦醇;老鼠

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Resveratrol prevents brain injury and neurogranin expression in rats exposed to gaseous formaldehyde

Objective To explore the effects of gaseous formaldehyde exposure on male Sprague-Dawley rat brain function and synaptic protein expression, as well as the neuroprotective effects of resveratrol. Methods Eighty-four male Sprague-Dawley rats were randomly divided into seven group with 12 in each group: control group (N), gaseous formaldehyde accumulation low concentration group (FL group), gaseous formaldehyde accumulation medium concentration group (FM group), gaseous formaldehyde accumulation high concentration group (FH group), gaseous formaldehyde accumulation low concentration + resveratrol group (FLR group), gaseous formaldehyde accumulation concentration + resveratrol group (FMR group) and gaseous formaldehyde accumulation high concentration + resveratrol group (FHR group). The animal model of gaseous formaldehyde accumulation was established by gas formaldehyde exposure method.Learning and memory function was tested by Morris water maze. Cortical and hippocampal neurogranin (Ng) protein expression in brain cortex and hippocampus was measured by Western blot. Results (1) Compared with the escape latency and original platform retention time of rats in group N ((19.75±4.08) s, (43.47±2.43) s), the escape latency of rats in FM and FH groups was prolonged ((25.52±2.98) s, (27.45±2.39) s, t=-4.97, -5.82; both P<0.01), the retention time of the original platform was shortened ((30.99±4.21) s, (18.70±2.58) s, t=10.24, 25.71; both P<0.01). Compared with the corresponding formaldehyde accumulation groups such as the FM and FH groups, the escape latency of the rats in the FMR and FHR groups was significantly longer ((21.37±3.80) s, (24.26±3.56) s, t=1.86, 3.93; both P<0.05), the retention time of the original platform was significantly prolonged ((40.33±3.63)s, (23.46±4.73)s, t=-7.50, -3.95; both P<0.01). (2) Compared with the expression of Ng in cortex and hippocampus of rats in group N ((0.99±0.03), (1.07±0.03)), the expression of Ng protein in cortex and hippocampus of rats in FM and FH groups was significantly reduced((0.62±0.06), (0.39±0.03), (0.77±0.09), (0.46±0.10), t=11.55, 14.08, 11.51, 13.17; all P<0.01). Compared with the corresponding formaldehyde accumulation groups such as the FM and FH groups, the expressions of Ng in the cortex and hippocampus of the rats in the FMR and FHR groups were significantly increased ((0.94±0.11), (0.48±0.04), (0.95±0.05), (0.60±0.09), t=-5.26, -2.09, -5.32, -2.21; all P<0.05). Conclusion Resveratrol can improve the learning and memory function of rats caused by the accumulation of gaseous formaldehyde.The mechanism may be related with the upregulation of Ng. Key words: Gaseous formaldehyde accumulation; Neurogranin; Learning and memory; Resveratrol; Rat

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中华行为医学与脑科学杂志

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期刊介绍： "Chinese Journal of Behavioral Medicine and Brain Science" (CN 37-1468/R, ISSN 1674-6554) is a national academic journal under the supervision of the National Health Commission, sponsored by the Chinese Medical Association and Jining Medical College. The journal was founded in June 1992 and was formerly known as "Chinese Journal of Behavioral Medicine" (1992-1993) and "Chinese Behavioral Medical Science" (1994-2008). In 2009, it was renamed "Chinese Journal of Behavioral Medicine and Brain Science" with the approval of the State Administration of Press, Publication, Radio, Film and Television. The purpose of "Chinese Journal of Behavioral Medicine and Brain Science" is to implement the health and health policies of the Party and the State, implement the principle of combining theory with practice and popularization and improvement, and reflect the major progress in the theory and practical application of behavioral medicine and brain science in my country. It publishes academic papers and scientific research results in the field of behavioral medicine and brain science in my country, and has columns such as monographs/reviews, basic research, clinical research, health prevention, methods and techniques, psychological behavior and evaluation, and systematic evaluation.