阿特拉津对两种重要经济海洋Pelecypoda物种的体内免疫毒性评价

Muhammed Zafar Iqbal AN
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引用次数: 0

摘要

在一项急性毒性试验中,两种具有商业意义的海洋Pelecypoda物种Perna viridis(绿色贻贝)和Paphia malabarica(短颈黄蛤)暴露于不同浓度的Atrataf(印度可买到的阿特拉津商业品牌)中。Atrataf对绿色假单胞菌和马拉巴里卡假单胞菌96 h LC50值分别为6.10 mg L-1和4.90 mg L-1。这项研究表明,随着暴露于亚致死浓度的阿特拉塔的剂量和持续时间的进一步增加,这两个物种的死亡率都显著增加。此外,在暴露于亚致死剂量的阿特拉塔14天后,通过使用台盼蓝排斥试验测量活血细胞来检测阿特拉津的免疫毒性潜力。在暴露于最高亚致死剂量的Atrataf 14天后,与对照组相比,活血细胞的百分比降至74.51(病毒Perna viridis)和78.39(马拉巴里氏巴氏杆菌)。由于血细胞是Pelecypoda免疫系统中最关键的细胞,血细胞计数的任何减少都将对免疫系统活动产生不利影响。这是首次研究和报道阿特拉津作为一种潜在的化合物,可诱导Pelecypoda的免疫毒性。事实上,这两个被研究的物种,即绿色Perna viridis和malabarica巴氏杆菌,在商业和生态上都很重要,它们的选择增加了研究的意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vivo Immunotoxicity Assessment of Atrazine in two Economically-Important Marine Pelecypoda Species
Two commercially significant marine Pelecypoda species named Perna viridis (green mussel) and Paphia malabarica (short neck yellow clam) were exposed to different concentrations of Atrataf (commercial brand of atrazine available in India) in an acute toxicity test. The 96 h LC50 values of Atrataf to P. viridis and P. malabarica were 6.10 mg L-1, and 4.90 mg L-1 respectively. This study showed that there is a significant increase in mortality in both species as the dose and duration of Atrataf exposure are increased further, exposure to sublethal concentrations of the Atrataf. Moreover, following 14 days of exposure to sublethal doses of Atrataf, the immunotoxic potential of atrazine was examined by measuring viable haemocytes using the Tryphan Blue Exclusion Assay. After 14 days of exposure to the highest sublethal doses of Atrataf, the percentage of viable hemocytes decreased to 74.51 (Perna viridis) and 78.39 (Paphia malabarica), relative to the control. Since Haemocytes are the most critical cells in the immune system of Pelecypoda, any decrease in the hemocyte count will have a detrimental impact on the immune system activities. This is the first study of its kind study to investigate and report atrazine as a potential compound, which can induce immunotoxicity in Pelecypoda. The fact that the two studied species of Perna viridis and Paphia malabarica, are both commercially and ecologically important, their selection adds to the study’s significance.
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来源期刊
Bioscience Biotechnology Research Communications
Bioscience Biotechnology Research Communications BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
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