酒渣鼻面部皮肤微生物群的分析与特征

IF 0.5 4区 医学 Q4 MICROBIOLOGY
Junying Li, Peng Cao, Quanzhong Liu, Weifeng Yao, Zhenhua Nie, Litao Zhang
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引用次数: 0

摘要

背景:有证据表明面部微生物群与几种皮肤状况之间存在关系。然而,对于酒渣鼻患者,面部皮肤微生物群的变化仍然未知。目的:本研究利用16S rDNA扩增子测序技术,探讨面部皮肤微生物群与酒渣鼻的相关性,并对酒渣鼻患者面部皮肤微生物群与健康对照进行分析和表征。方法:将27例酒渣鼻患者与25例健康对照进行配对。DNA是从参与者的鼻子、下巴、前额和双颊的皮肤拭子中提取的。采用Illumina MiSeq技术对16S rRNA基因V3V4区进行测序。使用α和β多样性检测面部皮肤微生物群的多样性。利用线性判别分析效应量(linear discriminant analysis effect size, LEfSe)对两组生物标志物进行定量研究。利用群落的系统发育调查,通过重建未观察到的状态,在属水平上进行了同源类群和京都基因百科全书的功能预测。结果:红斑痤疮患者面部皮肤微生物群的α多样性显著增加,β多样性在红斑痤疮患者与健康对照组之间存在显著差异。酒渣鼻患者面部皮肤微生物群落结构发生改变;然而,优势菌株与健康对照组相同,均为痤疮丙酸杆菌和表皮葡萄球菌。LEfSe结果显示酒渣鼻患者中含有丰富的黄单胞菌、不动杆菌和假单胞菌;然而,棒状杆菌、细细杆菌和嗜胃杆菌在健康对照中富集。酒渣鼻患者在膜转运、碳水化合物代谢、代谢性疾病、氨基酸转运与代谢、碳水化合物转运与代谢、转录、无机离子转运与代谢等途径的表达均显著降低。结论:与健康对照组相比,酒渣鼻患者面部皮肤微生物群多样性和群落结构发生变化,多种代谢途径表达下调,可能为酒渣鼻的监测、诊断和治疗提供新的策略方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis and Characterization of the Facial Skin Microbiota in Rosacea
Background: The evidence has shown the relationship between the microbiota of the face and several skin conditions. However, for rosacea patients, the changes in the facial skin microbiota still remain unknown. Objectives: This study was performed to explore the correlation between the facial skin microbiota and rosacea and analyze and characterize the facial skin microbiota of rosacea patients in comparison to healthy controls using 16S rDNA amplicon sequencing. Methods: A total of 27 rosacea patients and 25 healthy controls were matched. The DNA was extracted from participants’ skin swabs taken from the nose, chin, forehead, and bilateral cheeks. The V3V4 region of the 16S rRNA gene was sequenced using Illumina MiSeq technology. The diversity of the face skin microbiota was examined using alpha and beta diversity. Utilizing linear discriminant analysis effect size (LEfSe), the quantitative study of biomarkers in the two groups was carried out. Clusters of orthologous groups and Kyoto encyclopedia of genes and genomes function predictions were made at the genus level utilizing phylogenetic investigation of communities by reconstruction of unobserved states. Results: The alpha diversity of the facial skin microbiota increased significantly in rosacea patients, and beta diversity showed substantial differences between the rosacea and healthy control groups. The facial skin microbiota community structure changed in rosacea patients; however, the dominant strains were the same as in healthy controls, both being Propionibacterium acnes and Staphylococcus epidermidis. The LEfSe demonstrated that Xanthomonas, Acinetobacter, and Pseudomonas were enriched in the rosacea patients; nevertheless, Corynebacterium, Finegoldia, and Peptoniphilus were enriched in the healthy controls. The rosacea patients showed significantly decreased expression in the pathways of membrane transport, carbohydrate metabolism, metabolic diseases, amino acid transport and metabolism, carbohydrate transport and metabolism, transcription, and inorganic ion transport and metabolism. Conclusions: The facial skin microbiota diversity and community structure changed, and the expression of several metabolic pathways was downregulated in the rosacea patients in comparison to the healthy controls, which might outline new strategic methods for the surveillance, diagnosis, and treatment of rosacea.
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
56
审稿时长
6-12 weeks
期刊介绍: Jundishapur Journal of Microbiology, (JJM) is the official scientific Monthly publication of Ahvaz Jundishapur University of Medical Sciences. JJM is dedicated to the publication of manuscripts on topics concerning all aspects of microbiology. The topics include medical, veterinary and environmental microbiology, molecular investigations and infectious diseases. Aspects of immunology and epidemiology of infectious diseases are also considered.
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